Interactive effects of cortisol treatment and ambient seawater challenge on gill Na+,K+-ATPase and CFTR expression in two strains of Atlantic salmon smolts

Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway

“…Cortisol increased the CFTR-I transcript level in both FW and SW, whereas CFTR-II levels were only affected in the SW gill. This agrees with a report by Singer et al (2003) where cortisol treatment of FW Atlantic salmon stimulates CFTR-I but not CFTR-II mRNA levels. The CFTR-I isoform is also upregulated during smoltification and after SW-transfer in salmon, while CFTR-II is only transiently affected under these conditions (reviewed by Marshall & Singer 2002; T. Nilsen, Univ.…”

Optimizing de novo transcriptome assembly and extending genomic resources for striped catfish (Pangasianodon hypophthalmus)

“…The two isoforms of CFTR have been shown to play a role in adaptation of salmon smolts following transfer to sea water (Singer et al, 2002). Gill CFTR activity increases after transfer to seawater in many fish species including in killifish (Marshall et al, 1999;Marshall et al, 2002), Hawaiian goby Stenogobius hawaiiensis , Atlantic salmon (Singer et al, 2003) and Mozambique tilapia (Hiroi et al, 2005;Ouattara et al, 2009).…”

Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+-ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones

“…In killifish, the evidence is correlative and based on measurements of plasma cortisol and CFTR expression (nZ1) during a time course of SW acclimation (Marshall et al 1999). In FW Atlantic salmon, CFTR-I mRNA levels were unaffected by 5-day cortisol implants; however, when fish subsequently were sham transferred to FW, CFTR-I transcript levels increased after 24 h (Singer et al 2003). Interestingly, in mouse endometrium, hyperaldosteronaemia induced by a low-sodium diet depresses CFTR and increases ENaC channel expression (Tsang et al 2004).…”

“…On the other hand, information on the endocrine regulation of gill CFTR expression is scarce. The promoter region of mammalian and killifish CFTR contains glucocorticoidbinding sites (Schulte & Keir 2003, McCarthy & Harris 2005, and there is evidence that cortisol is an important component of CFTR regulation in killifish (Marshall & Singer 2002) and Atlantic salmon (Singer et al 2003).…”