Interactive effects of algicidal efficiency of Bacillus sp. B50 and bacterial community on susceptibility of Microcystis aeruginosa with different growth rates

Shao, Jihai; He, Yaxian; Chen, Anwei; Peng, Liang; Luo, Si; Wu, Genyi; Zou, Huiling; Li, Renhui

doi:10.1016/j.ibiod.2014.10.013

Cited by 25 publications

(6 citation statements)

References 16 publications

(20 reference statements)

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“…Most studies conducted so far have considered chlorophyll content to assess the alginolytic effects of algicidal bacteria (Su et al 2007; Huang et al 2013; Shao et al 2015), although it is debated whether chlorophyll content can be used to assess alginolytic effects after lysis of algal cells by algicidal bacteria. In the present study, the algicidal strain CZBC1 showed direct alginolytic effects on O. chlorina and O. tenuis and indirect alginolytic effects on O. planctonica , and we directly determined algal cell count to more accurately assess alginolytic effects.…”

Section: Discussionmentioning

confidence: 99%

Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica

et al. 2019

AMB Expr

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Frequent harmful cyanobacteria blooms limit the sustainable development of aquaculture. Algicidal bacteria can efficiently control harmful algae without secondary pollution. The algicidal bacteria CZBC1 can lyse Oscillatoria spp. and other harmful cyanobacteria, but its effector mechanism and algicidal threshold are unknown. In this study, we examined the algicidal effect of CZBC1 on O. chlorina, O. tenuis, and O. planctonica by microscopic enumeration and scanning electron microscopy observation. Then, we examined the alginolytic effects of CZBC1 (concentrations 103–106 colony forming units (cfu)/mL) on these three species (concentrations 103–106 cells/mL) to determine the effective concentrations of CZBC1 for Oscillatoria spp. alginolysis. Results showed that CZBC1 can directly lyse O. chlorina and O. tenuis but indirectly lyse O. planctonica. When the initial concentration of CZBC1 was 106 cfu/mL, alginolytic effects were high for all three species at all concentrations, and the alginolytic rate could reach 100% in 3–9 days. When the initial concentration of CZBC1 was lower (103 cfu/mL), its inhibitory effects were delayed by 2–5 days, but the cell counts were significantly decreased compared with the control, evidencing significant alginolysis. In addition, the higher the concentration of the algicidal bacteria suspension, the more significant the alginolytic effects. Our results indicate that CZBC1 has different alginolytic mechanisms for O. chlorina, O. tenuis, and O. planctonica, and that different initial concentrations of CZBC1 have different alginolytic effects on these algal species.

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Section: Discussionmentioning

confidence: 99%

Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica

et al. 2019

AMB Expr

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“…One study showed that the culture temperature and algal density of Microcystis aeruginosa affected its susceptibility to algicidal bacteria (Su et al 2016 ). Another study reported a linear relationship between algicidal efficiency and the growth rate of M. aeruginosa , and suggested environmental conditions influencing the growth of M. aeruginosa could impact the efficacy of algicidal bacteria (Shao et al 2015 ). Although the approaches showed some improvement in algicidal efficacy, the investigated environmental parameters suffer inherent limitations for practical applications because parameters such as temperature in the lake or ocean is difficult to be controlled or readily manipulated.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics analysis of Pseudomonas chlororaphis JK12 algicidal activity under aerobic and micro-aerobic culture condition

et al. 2018

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Utilization of algicidal bacteria as a biological agent have been receiving significant interest for controlling harmful algal blooms. While various algicidal bacterial strains have been identified, limited studies have explored the influence of bacterial culture conditions on its algicidal activity. Here, the effect of oxygen on the algicidal activity of a novel bacterium JK12, against a model diatom, Phaeodactylum tricornutum (P. tricornutum) was studied. Strain JK12 showed high algicidal activity against P. tricornutum and was identified as Pseudomonas chlororaphis (P. chlororaphis) by 16S ribosomal RNA gene analysis. JK12 culture supernatant exhibited strong algicidal activity while washed JK12 cells showed no obvious activity, indicating that JK12 indirectly attacks algae by secreting extracellular algicidal metabolites. Micro-aerobic culture condition dramatically enhanced the algicidal activity of JK12 by 50%, compared to that cultured under aerobic condition in 24 h. Extracellular metabolomic profiling of JK12 using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry analysis revealed significantly higher amounts of allantoic acid, urocanic acid, cytidine 2′,3′-cyclic phosphate, uridine 2′,3′-cyclic phosphate, and chlorinated tryptophan in the micro-aerobic culture. This is the first report to demonstrate the important role of oxygen on the algicidal activity of a non-pathogenic strain P. chlororaphis. In addition, the metabolomics analysis provided insights into the algicidal mechanism of P. chlororaphis.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0660-x) contains supplementary material, which is available to authorized users.

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“…It has been reported that the algicidal activity of bacteria depends on bacterial concentration and algal cell density ( Tian et al, 2012 ; Kong et al, 2020 ; Al-Hakimi et al, 2020 ). For instance, Shao et al (2015) found that Bacillus sp. B50 showed an algicidal activity when the bacterial concentration reached 1.9 × 10 6 CFU/mL, while the algicidal activity was lost at a cell concentration below 1.9 × 10 5 CFU/mL.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a high-efficiency algicidal bacterium Pseudoalteromonas sp. LD-B6 against the harmful dinoflagellate Noctiluca scintillans

Yin¹,

Xu²,

Chen³

et al. 2022

Front. Microbiol.

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The dinoflagellate Noctiluca scintillans is a harmful algal species that is globally distributed and poses a certain threat to marine ecosystems. Recent research has shown that the application of algicidal bacteria is a promising method to prevent and control such harmful algal blooms (HABs), given its advantages of safety and efficiency. In this study, a strain of algicidal bacterium LD-B6 with high efficiency against N. scintillans was isolated from the coastal waters of Lianyungang, China. 16S rDNA sequence analysis showed that the strain LD-B6 belongs to the genus Pseudoalteromonas. Furthermore, the algicidal effect of LD-B6 on N. scintillans was investigated. The results showed that strain LD-B6 exerted strong algicidal activity against N. scintillans. After 12 h of bacterial culture addition to algal cultures at a 2% final volume rate, the algicidal activity reached 90.5%, and the algicidal activity of LD-B6 was influenced by the density of N. scintillans. In addition, the algicidal bacterium LD-B6 was found to indirectly lyse algal cells by secreting extracellular compounds. These algicidal compounds were stable, indicating that they are not proteins. Importantly, strain LD-B6 was broadly general, showing varying degrees of lysing effects against five of the six algal species tested. On the basis of the described studies above, the algicidal powder was also initially developed. In summary, the isolated bacterial strain LD-B6 shows the potent algicidal capability to serve as a candidate algicidal bacterium against N. scintillans blooms.

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Interactive effects of algicidal efficiency of Bacillus sp. B50 and bacterial community on susceptibility of Microcystis aeruginosa with different growth rates

Cited by 25 publications

References 16 publications

Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica

Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica

Metabolomics analysis of Pseudomonas chlororaphis JK12 algicidal activity under aerobic and micro-aerobic culture condition

Isolation and characterization of a high-efficiency algicidal bacterium Pseudoalteromonas sp. LD-B6 against the harmful dinoflagellate Noctiluca scintillans

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