Interactions of the HSM3 gene with genes initiating homologous recombination repair in yeast Saccharomyces cerevisiae

Chernenkov, A. Yu.; Fedorov, D. V.; Gracheva, L.M.; Evstuhina, T. A.; Ковальцова, С В; Peshekhonov, V. T.; Федорова, И. В.; Korolev, V. G.

doi:10.1134/s1022795412020056

Cited by 6 publications

(11 citation statements)

References 23 publications

(30 reference statements)

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“…Based on the data obtained, it can be concluded that hsm3∆ and hif1∆ mutations do not affect NER. rad54∆ and rad52∆ mutants are weakly sensitive to UV, which corresponds to the previously obtained data [38]. Earlier, we have shown that hsm3∆ mutation does not change the UV sensitivity of rad52∆ mutant [38].…”

Section: Resultssupporting

confidence: 91%

“…rad54Δ and rad52Δ mutants are weakly sensitive to UV, which corresponds to the previously obtained data [ 38 ]. Earlier, we have shown that hsm3Δ mutation does not change the UV sensitivity of rad52Δ mutant [ 38 ]. Double hif1Δ rad52Δ mutant had the same sensitivity as single radiosensitive mutant ( Figure 1 B).…”

Section: Resultssupporting

confidence: 91%

“…These mutants did not change significantly the rate of spontaneous death of cells compared to the wild-type strain (Table 2). According to our research and the data presented in work [38], in the double hif1∆ rad52∆ and hsm3∆ rad52∆ mutants, hif1∆ and hsm3∆ mutants were equally epistatized to recombination-deficient mutants. These data support the hypothesis advanced earlier that hsm3∆ mutation (maybe hif1∆) leads to the destabilization of the D-loop during the post-replicative repair [38,43] and thus reduces the load on the path of the recombination repair, which is blocked in rad52∆ mutant.…”

Section: Resultssupporting

confidence: 67%

“…In comparative experiments previously described, LMG-351 ( MATα ade2Δ-248 leu2-3,112 ura3-160,188 trp1 rad1::LEU2 ) [ 29 ], LMG-352 ( MATα ade2Δ-248 leu2-3,112 ura3-160,188 trp1 rad2::TRP1 ) [ 37 ], 10-CAY-3031 ( MATα ade2Δ-248 leu2-3,112 ura3-160,188 trp1 rad52::URA3 ) [ 38 ], and 13-CAY-5-3031 ( MATα ade2Δ-248 leu2-3,112 ura3-160,188 trp1 hsm3::KanR rad52::URA3 ) [ 38 ] strains were used.…”

Section: Methodsmentioning

confidence: 99%

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Genetic Analysis of the Hsm3 Protein Function in Yeast Saccharomyces cerevisiae NuB4 Complex

Evstyukhina

Алексеева

Fedorov

et al. 2021

Genes

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In the nuclear compartment of yeast, NuB4 core complex consists of three proteins, Hat1, Hat2, and Hif1, and interacts with a number of other factors. In particular, it was shown that NuB4 complex physically interacts with Hsm3p. Early we demonstrated that the gene HSM3 participates in the control of replicative and reparative spontaneous mutagenesis, and that hsm3Δ mutants increase the frequency of mutations induced by different mutagens. It was previously believed that the HSM3 gene controlled only some minor repair processes in the cell, but later it was suggested that it had a chaperone function with its participation in proteasome assembly. In this work, we analyzed the properties of three hsm3Δ, hif1Δ, and hat1Δ mutants. The results obtained showed that the Hsm3 protein may be a functional subunit of NuB4 complex. It has been shown that hsm3- and hif1-dependent UV-induced mutagenesis is completely suppressed by inactivation of the Polη polymerase. We showed a significant role of Polη for hsm3-dependent mutagenesis at non-bipyrimidine sites (NBP sites). The efficiency of expression of RNR (RiboNucleotid Reducase) genes after UV irradiation in hsm3Δ and hif1Δ mutants was several times lower than in wild-type cells. Thus, we have presented evidence that significant increase in the dNTP levels suppress hsm3- and hif1-dependent mutagenesis and Polη is responsible for hsm3- and hif1-dependent mutagenesis.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 67%

Section: Methodsmentioning

confidence: 99%

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Genetic Analysis of the Hsm3 Protein Function in Yeast Saccharomyces cerevisiae NuB4 Complex

Evstyukhina

Алексеева

Fedorov

et al. 2021

Genes

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“…S. cerevisiae strains used in this work are described in Table 1 . The xrs2Δ (2ETA-3031) mutant were obtained from the previously described 11D-3031 ( MATα ade2Δ-248 leu2-3,112 ura3-160,188 trp1 ) strain by gene replacement (Fedorova et al 2004 ; Chernenkov et al 2012a , b ). The 11D-3031 strain was transformed with modules, and the transformants were selected on plates with YEPD containing 30 mg/l nourseothricin and on plates with selective media without uracil, respectively.…”

Section: Methodsmentioning

confidence: 99%

Participation of the HIM1 gene of yeast Saccharomyces cerevisiae in the error-free branch of post-replicative repair and role Polη in him1-dependent mutagenesis

et al. 2020

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In eukaryotes, DNA damage tolerance (DDT) is determined by two repair pathways, homologous repair recombination (HRR) and a pathway controlled by the RAD6-epistatic group of genes. Monoubiquitylation of PCNA mediates an error-prone pathway, whereas polyubiquitylation stimulates an error-free pathway. The error-free pathway involves components of recombination repair; however, the factors that act in this pathway remain largely unknown. Here, we report that the HIM1 gene participates in error-free DDT. Notably, inactivation RAD30 gene encoding Polη completely suppresses him1-dependent UV mutagenesis. Furthermore, data obtained show a significant role of Polη in him1-dependent mutagenesis, especially at non-bipyrimidine sites (NBP sites). We demonstrate that him1 mutation significantly reduces the efficiency of the induction expression of RNR genes after UV irradiation. Besides, this paper presents evidence that significant increase in the dNTP levels suppress him1-dependent mutagenesis. Our findings show that Polη responsible for him1-dependent mutagenesis.

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Spontaneous mutation rate changes in Saccharomyces cerevisiae at combinations of hsm3 and hsm6 mutations with rad52 mutation

et al. 2014

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Interactions of the HSM3 gene with genes initiating homologous recombination repair in yeast Saccharomyces cerevisiae

Cited by 6 publications

References 23 publications

Genetic Analysis of the Hsm3 Protein Function in Yeast Saccharomyces cerevisiae NuB4 Complex

Genetic Analysis of the Hsm3 Protein Function in Yeast Saccharomyces cerevisiae NuB4 Complex

Participation of the HIM1 gene of yeast Saccharomyces cerevisiae in the error-free branch of post-replicative repair and role Polη in him1-dependent mutagenesis

Spontaneous mutation rate changes in Saccharomyces cerevisiae at combinations of hsm3 and hsm6 mutations with rad52 mutation

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