Interactions of the apolipoprotein C-III 3238C>G polymorphism and alcohol consumption on serum triglyceride levels

Abstract

Background Both apolipoprotein (Apo) C-III gene polymorphism and alcohol consumption have been associated with increased serum triglyceride (TG) levels, but their interactions on serum TG levels are not well known. The present study was undertaken to detect the interactions of the ApoC-III 3238C>G (rs5128) polymorphism and alcohol consumption on serum TG levels. Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the ApoC-III 3238C>G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Interactions of the ApoC-III 3238C>G genotype and alcohol consumption was assessed by using a cross-product term between genotypes and the aforementioned factor. Results Serum total cholesterol (TC), TG, high-density lipoprotein cholesterol (HDL-C), ApoA-I and ApoB levels were higher in drinkers than in nondrinkers ( P < 0.05-0.001). There was no significant difference in the genotypic and allelic frequencies between the two groups. Serum TG levels in nondrinkers were higher in CG genotype than in CC genotype ( P < 0.01). Serum TC, TG, low-density lipoprotein cholesterol (LDL-C) and ApoB levels in drinkers were higher in GG genotype than in CC or CG genotype ( P < 0.01 for all). Serum HDL-C levels in drinkers were higher in CG genotype than in CC genotype ( P < 0.01). Serum TC, TG, HDL-C and ApoA-I levels in CC genotype, TC, HDL-C, ApoA-I levels and the ratio of ApoA-I to ApoB in CG genotype, and TC, TG, LDL-C, ApoA-I and ApoB levels in GG genotype were higher in drinkers than in nondrinkers ( P < 0.05-0.01). But the ratio of ApoA-I to ApoB in GG genotype was lower in drinkers than in nondrinkers ( P < 0.01). Multivariate logistic regression analysis showed that the levels of TC, TG and ApoB were correlated with genotype in nondrinkers ( P < 0.05 for all). The levels of TC, LDL-C and ApoB were associated with genotype in drinkers ( P < 0.01 for all). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index in both groups. Conclusions This study suggests that the ApoC-III 3238CG heterozygotes benefited more from alcohol consumption than CC and GG homozygotes in increasing serum levels of HDL-C, ApoA-I, and the ratio of ApoA-I to ApoB, and lowering serum levels of TC and TG.

Activation of NLRP3 inflammasome by cholesterol crystals in alcohol consumption induces atherosclerotic lesions

“…Our findings showed that lowering of cholesterol levels by ALC+AVS concomitantly prevented cell invasion in the vasculature, thereby minimizing the early event of atherosclerosis. This was supported by reduction of serum cholesterol levels, cholesterol build-up in different size of the vessels, thickening of arterial/capillary walls, and intracranial blood pressure in alcohol consumptionfollowing ALC/Lipitor ® administration.The increase in serum LDL cholesterol levels associated with alcohol diet ingested animals in this study is similar to serum LDL cholesterol levels observed in human alcoholics[31,32]. …”

Interactions of several single nucleotide polymorphisms and high body mass index on serum lipid traits

“…On ordinary days, they are vegetarians [7][8][9]. Third, it is clearly established that serum lipid levels are regulated by multiple environmental and genetic factors, and their interactions [7][8][9][10][11][12]. Although we have detected the interactions of 10 SNPs and high BMI on serum lipid levels in this study, there are still many unmeasured environmental and genetic factors and their interactions.…”

“…Genomic DNA was extracted from the peripheral blood leukocytes by the phenol-chloroform method [10][11][12]. Genotyping of rs2066715, rs1044925, low-density lipoprotein receptor (LDL-R) Avak, rs2070895, rs2000813, rs1801133, rs3757354, rs505151, rs2016520, and rs5888 was performed using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).…”

“…Dyslipidemia such as elevated levels of total cholesterol (TC) [1], triglyceride (TG) [2], low-density lipoprotein cholesterol (LDL-C) [3], and apolipoprotein (Apo) B [4], together with decreased levels of ApoA1 [4] and high-density lipoprotein cholesterol (HDL-C) [5] has become a serious public health problem in many countries because of its high prevalence and a causal relationship with serious medical condition such as coronary artery disease (CAD), hypertension, and stroke [6]. It is well known that dyslipidemia is a complex trait caused by multiple environmental and genetic factors and their interactions [7][8][9][10][11][12]. Although the associations between overweight/ obesity and dyslipidemia have been well documented [13][14][15][16][17], information on the interactions between single nucleotide polymorphisms (SNPs) and high body mass index (BMI) on serum lipid profiles is still scarce [18][19][20][21].…”

Interactions Between the Apolipoprotein A1/C3/A5 Haplotypes and Alcohol Consumption on Serum Lipid Levels

“…Numerous single nucleotide polymorphisms (SNPs) and alcohol consumption have been associated with lipid metabolism and variation in serum lipid profiles. The interactions between several SNPs and alcohol consumption on serum lipid levels and CAD (Corella et al., ; Jensen et al., ; Liu et al., ; Ruixing et al., ; Yin et al., ) have also been reported previously.…”