Interactions of some local anesthetics and alcohols with membranes

Frangopol, Petre T.; Mihăilescu, Dan

doi:10.1016/s0927-7765(01)00153-9

Cited by 49 publications

(37 citation statements)

References 83 publications

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“…The different liposolubilities of gases result in different changes of the physiological, physical and electrical properties of cell membranes (Seeman and Hills and Ray, 1977;D'Agostino et al, 2009), partly similar to those already known from alcohols and anesthetics (Frangopol and Mihailescu, 2001;Yun et al, 2002).…”

Section: Introductionmentioning

confidence: 81%

The effect of hyperbaric air on the electric activity of neuronal in vitro networks

Stubbe

Nissen

Schroeder

et al. 2015

Biosensors and Bioelectronics

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Breathing hyperbaric air or gas mixtures, for example during diving or when working underwater is known to alter the electrophysiological behavior of neuronal cells, which may lead to restricted cognition. During the last few decades, only very few studies into hyperbaric effects have been published, especially for the most relevant pressure range of up to 10 bar. We designed a pressurized measuring chamber to record pressure effects on the electrical activity of neuronal networks formed by primary cells of the frontal cortex of NMRI mice. Electrical activity was recorded with multi-electrode arrays (MEAs) of glass neuro chips while subjected to a step-by-step pressure increase from atmospheric pressure (1 bar) to 2 and 4 bar, followed by a decompression to 1 bar, in order to record recovery effects. The effects of pressure on the total spike rates (TSRs), which were averaged from at least 45 chips, were detected in two cell culture media with different compositions. In a DMEM medium with 6% horse serum, the TSR was increased by 19% after a pressure increase to 2 bar and remained stable at 4 bar. In NMEM medium with 2% B27, the TSR was not altered by a pressure increase to 2 bar but increased by 9% at 4 bar. After decompression to 1 bar, the activities decreased to 76% and 101% of their respective control levels in the two media. MEA recordings from neuronal networks in miniaturized hyperbaric measuring chambers provide new access for exploring the neuronal effects of hyperbaric breathing gases.

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Section: Introductionmentioning

confidence: 81%

The effect of hyperbaric air on the electric activity of neuronal in vitro networks

Stubbe

Nissen

Schroeder

et al. 2015

Biosensors and Bioelectronics

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“…While the blockade of voltage-gated sodium channels embedded in plasma membranes is the primary mode of action of local anesthesia, it is also caused by the interaction between drugs and membrane lipids. Local anesthetics induce changes in physicochemical properties, such as the fl uidization or disordering of the lipid bilayers surrounding receptors and ion channels, resulting in the modifi cation of their activities through the conformational alteration of membrane proteins [1]. These cellular events are determined by the hydrophobic interactions of local anesthetics with lipid components.…”

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confidence: 99%

Peroxynitrite affects lidocaine by acting on membrane-constituting lipids

et al. 2008

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Inflammation frequently decreases local anesthetic effects, especially in dental anesthesia in patients with pulpitis and periodontitis. The pharmacokinetics and the mode of action of local anesthetics are closely associated with the hydrophobic interactions between these drugs and lipid bilayers that change the membrane physicochemical property, fluidity. A lipid oxidant, peroxynitrite, is produced by inflammatory cells, and it may act on nerve cell membranes and affect anesthetic efficacy. With respect to this speculated action, we addressed whether peroxynitrite acted on membrane-constituting lipids to decrease the membrane interactivity of lidocaine. Membrane fluidity changes were determined by measuring the fluorescence polarization of liposomes prepared with different phospholipids. Peroxynitrite (0.1-50 microM) rigidified nerve-cell model membranes consisting of unsaturated phospholipids, as well as liposomal membranes consisting of 1,2-dioleoylphosphatidylcholine and 1-stearoyl-2-arachidonylphosphatidylcholine, but peroxynitrite did not rigidify 1, 2-dipalmitoylphosphatidylcholine liposomal membranes. The pretreatment of nerve-cell model membranes with peroxynitrite (0.1-50 microM) decreased the membrane-fluidizing effects of lidocaine (5.0 mg x ml(-1)) to 63%-86% of the control (not treated with peroxynitrite) depending on the peroxynitrite concentration. As one of the mechanisms of the local anesthetic failure associated with inflammation, inflammatory peroxynitrite may affect local anesthesia by acting on membrane-constituting unsaturated phospholipids.

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“…Langmuir films have also been widely used to study the interaction and penetration of soluble molecules (anesthetics [48], proteins and peptides [49][50][51][52][53][54][55][56], vitamins [57], etc.) in membranes.…”

Section: Experimental Approaches On Monolayersmentioning

confidence: 99%

Lipid Monolayers at the Air–Water Interface

Wilke

2014

Advances in Planar Lipid Bilayers and Liposomes

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Interactions of some local anesthetics and alcohols with membranes

Cited by 49 publications

References 83 publications

The effect of hyperbaric air on the electric activity of neuronal in vitro networks

The effect of hyperbaric air on the electric activity of neuronal in vitro networks

Peroxynitrite affects lidocaine by acting on membrane-constituting lipids

Lipid Monolayers at the Air–Water Interface

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