Interactions of Serum Derived Proteins with Sub‐Micrometer Structured Surfaces

Abstract

This work presents the development and optimization of two fabrication methods that combine plasma polymerization and electron beam lithography techniques for producing chemical patterns at the sub-micro scale. The first method uses sacrificial resist as mask to produce the functionalization of the bioadhesive areas and the second method consists in the direct EBL writing of adhesive regions on a non-adhesive plasma deposited PEO-like film. The produced patterned surfaces exhibit high binding capacity as tested by Surface Plasmon Resonance Imaging with Human Serum Albumin. Furthermore, we show that adsorption on the structured surfaces is similar to that observed on a flat surface with a direct proportionality to the active area. This work shows the efficiency and suitability of the developed chemical patterning techniques and their high potential applicability for the study of protein surface interactions and miniaturized biosensing device development.

Modulation of surface bio-functionality by using gold nanostructures on protein repellent surfaces

“…It is clear that the e-beam treatment prevents the adsorption of the Au NPs on the PEOlike coatings, the surface coverage decreases from 13.3% on the as-deposited area to 1.8% for the 12 pC mm À2 e-beam irradiated sample and to 0.09% for the 85 pC mm À2 e-beam irradiated sample. Our previous work 17 showed that the main effect of the e-beam treatment on PEO-like coatings is the increase in refractive index of the lm, which indicates an increase of the crosslinking between the polymeric chains.…”

“…Synthesis of 15 nm sized Au NPs was carried out by modication of the procedure described by Turkevich et al, 17 in which the gold nanoparticles are produced by the reduction of the gold salt by the sodium citrate that acts as a reducing agent and stabilizer. In this work, the solution was heated up using a specialized microwave apparatus (Discover S by CEM corporation) to ensure a highly reproducible rapid heating.…”

Sticky superhydrophobic hard nanofibers from soft matter

“…2,2-Bis(5-methylhexyl)propanediol (3b). Yield 95%; colourless liquid; d H (200 MHz, CDCl 3 , ppm): 3.57 (s, 4H), 2.00 (s, 2H), 1.51 (m, 2H), 1.22 (m, 16H), 0.85 (d, 3 J HH ¼ 6.6 Hz, 12H).…”

“…Various hydrophobic substituents can be graed on the monomers to modify the intrinsic polymer hydrophobicity. For the growth of nanobers, polyaniline, 26,27 polypyrrole, [28][29][30] poly (3,4-ethylenedioxythiophene) (PEDOT) [31][32][33][34][35] or poly (3,4-propylenedioxythiophene) (PProDOT) [36][37][38] derivatives have been described in the literature. One of the advantages of using ProDOT derivatives is the various possible positions for controlled substitution by hydrophobic substituents.…”

“…Tuning the characteristics and hydrophilic/hydrophobic properties of nanofibers grown on surfaces is crucial for various applications such as cell, 1-2 protein [3][4] or bacteria adhesion, 5 tissue engineering, 6 membranes, 7 or encapsulation. 8 In the case of generation of superhydrophobicity, the wetting properties of surfaces [9][10] containing nanofibers highly depend on their intrinsic hydrophobicity, their characteristics (length, diameter), their orientation to the surface (horizontally, vertically) as well as the distance between them.…”