Interactions of rhizobia with rice and wheat

Webster, Gordon; Gough, Clare; Vasse, Jacques; Batchelor, C. A.; O’Callaghan, K. J.; Kothari, S. L.; Davey, M. R.; Denarié, Jean; Cocking, E. C.

doi:10.1007/978-94-011-5744-5_11

Cited by 23 publications

(20 citation statements)

References 38 publications

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“…and A. caulinodans also infect plants through cracks at the base of the root primordia, not only in the roots but also in the stems of leguminous plants. They are also known to enter the roots of non-leguminous plants such as rice and wheat via cracks 6,27,38) . In the present study, nifH sequences homologous to Bradyrhizo- Clones that exceeded 99% base sequence similarity with each other were divided into different groups and a representative clone was chosen from each group.…”

Section: Resultsmentioning

confidence: 99%

Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes (Ipomoea batatas L.)

et al. 2008

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We examined the nitrogenase reductase (nifH) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (Ipomoea batatas L.) by reverse transcription (RT)-PCR. Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. Sequence analysis revealed that these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database. Investigation of the nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteria. These results suggest that bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes.

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Section: Resultsmentioning

confidence: 99%

Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes (Ipomoea batatas L.)

et al. 2008

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“…In fact, many plants such as Saccharum officinarum [20,47], Sorghum bicolor L. [48], Triticum aestivum [33,49], Oryza sativa L. [33,34,50,51], Zea mays L. [28,52], Gossypium sp. [53,54], Vitis vinifera [25,55], Lactuca sativa [28], Picea -abies [56], Coffea arabica [57], Helianthus petiolaris [11], Musa spp.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Variations of Endophytic Bacteria Associated with Carob Tree (Ceratonia Siliqua L.)

Konaté¹

2014

AJLS

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Abstract:We aimed to characterize 83 endophytic bacteria isolated from roots (73 isolates coded IRC) and epicotyls (11 isolates coded IEC) of young Carob (Ceratonia siliqua L.) seedlings. These seedlings were obtained from seeds collected in several regions in Morocco. 30 IRC and 4 IEC were selected on the basis of PCR-Pep for the further analyses. All the strains exhibited a wide tolerance to NaCl and 30 % tolerated well concentration up to 11 % NaCl. Strains showed also a wide tolerance to the variable pH. 60 % of strains grew well at pH4. Most of the strains were resistant to different antibiotics but were sensitive to kanamycin and tetracycline. The strains showed a resistance to heavy metals except mercury chloride that was toxic at a low concentration 50 µg.ml-1. According to their phenotypical features, the associative bacteria were very similar to eight symbiotic bacteria previously identified by Missbah in 1996 and used in this work.

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“…As we detected a nitrogen-fixing activity in inoculated rice plants, nitrogen fixation could contribute to this plant growth-promoting response. Nitrogenase activity had previously been detected in wheat inoculated with Azorhizobium strain ORS571, but only when succinate was added to the plant growth medium (42). Photosynthetic Bradyrhizobium strains are known to fix nitrogen under free-living conditions (2).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the infection process in the monocotyledonous plant O. breviligulata by the same photosynthetic bradyrhizobia seems to be very similar to the first stages of infection in the leguminous dicotyledonous plant Aeschynomene. Webster et al (42) showed that colonization of rice and wheat roots by A. caulinodans strain ORS571 was nod gene independent. With the photosynthetic Bradyrhizobium strain ORS278, one hypothesis could also be that, in both A. sensitiva and O. breviligulata, expression of nod genes is not necessary for the first steps of infection involving primitive "crack entry" and direct intercellular invasion.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

Chaintreuil

Giraud

Prin

et al. 2000

Appl Environ Microbiol

248

139

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We investigated the presence of endophytic rhizobia within the roots of the wetland wild rice Oryza breviligulata, which is the ancestor of the African cultivated rice Oryza glaberrima. This primitive rice species grows in the same wetland sites as Aeschynomene sensitiva, an aquatic stem-nodulated legume associated with photosynthetic strains of Bradyrhizobium. Twenty endophytic and aquatic isolates were obtained at three different sites in West Africa (Senegal and Guinea) from nodal roots of O. breviligulata and surrounding water by using A. sensitiva as a trap legume. Most endophytic and aquatic isolates were photosynthetic and belonged to the same phylogenetic Bradyrhizobium/Blastobacter subgroup as the typical photosynthetic Bradyrhizobium strains previously isolated from Aeschynomene stem nodules. Nitrogen-fixing activity, measured by acetylene reduction, was detected in rice plants inoculated with endophytic isolates. A 20% increase in the shoot growth and grain yield of O. breviligulata grown in a greenhouse was also observed upon inoculation with one endophytic strain and one Aeschynomene photosynthetic strain. The photosynthetic Bradyrhizobium sp. strain ORS278 extensively colonized the root surface, followed by intercellular, and rarely intracellular, bacterial invasion of the rice roots, which was determined with a lacZ-tagged mutant of ORS278. The discovery that photosynthetic Bradyrhizobium strains, which are usually known to induce nitrogen-fixing nodules on stems of the legume Aeschynomene, are also natural true endophytes of the primitive rice O. breviligulata could significantly enhance cultivated rice production.

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Interactions of rhizobia with rice and wheat

Cited by 23 publications

References 38 publications

Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes (Ipomoea batatas L.)

Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes (Ipomoea batatas L.)

Phenotypic Variations of Endophytic Bacteria Associated with Carob Tree (Ceratonia Siliqua L.)

Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

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