Interactions of rhizobia with rice and wheat

Webster, Gordon; Gough, Clare; Vasse, Jacques; Batchelor, C. A.; O’Callaghan, K. J.; Kothari, S. L.; Davey, M. R.; Denarié, Jean; Cocking, E. C.

doi:10.1007/978-94-011-7113-7_11

Cited by 31 publications

(20 citation statements)

References 47 publications

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“…It has been reported that non‐legumes also reacted to the presence of rhizobia in the rhizosphere (Plazinsky et al 1985, Terouchi and Syono 1990). In rice and wheat, for example, rhizobia colonized the root surface and intercellular spaces between injured epidermal cells (Reddy et al 1997, Webster et al 1997). Furthermore, Nod factors can be perceived by non‐legumes, such as tomato, as indicated by alkalinization of the medium in cell cultures (Staehelin et al 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, rhizobia share characteristics with plant growth promoting rhizobacteria that colonize the root surface. In the presence of rhizobia, growth of maize, lettuce, radish, rice and wheat was increased (Antoun et al 1998, Chabot et al 1996, Höflich et al 1995, Webster et al 1997). In addition to this plant growth promoting effect, rhizobia have also been associated with disease suppressive effects in legumes (Avis et al 2008, Bardin et al 2003, Chakraborty and Chakraborty 1989, Chakraborty and Purkayastha 1984, Elbadry et al 2006, Huang et al 2007, Siddiqui et al 2007, Tu 1978).…”

Section: Introductionmentioning

confidence: 99%

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The Rhizobium sp. strain NGR234 systemically suppresses arbuscular mycorrhizal root colonization in a split-root system of barley (Hordeum vulgare)

Khaosaad

Staehelin

Steinkellner

et al. 2010

Physiologia Plantarum

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Nitrogen-fixing bacteria (rhizobia) form a nodule symbiosis with legumes, but also induce certain effects on non-host plants. Here, we used a split-root system of barley to examine whether inoculation with Rhizobium sp. strain NGR234 on one side of a split-root system systemically affects arbuscular mycorrhizal (AM) root colonization on the other side. Mutant strains of NGR234 deficient in Nod factor production (strain NGRΔnodABC), perception of flavonoids (strain NGRΔnodD1) and secretion of type 3 effector proteins (strain NGRΩrhcN) were included in this study. Inoculation resulted in a systemic reduction of AM root colonization with all tested strains. However, the suppressive effect of strain NGRΩrhcN was less pronounced. Moreover, levels of salicylic acid, an endogenous molecule related to plant defense, were increased in roots challenged with rhizobia. These data indicate that barley roots perceived NGR234 and that a systemic regulatory mechanism of AM root colonization was activated. The suppressive effect appears to be Nod factor independent, but enhanced by type 3 effector proteins of NGR234.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Rhizobium sp. strain NGR234 systemically suppresses arbuscular mycorrhizal root colonization in a split-root system of barley (Hordeum vulgare)

Khaosaad

Staehelin

Steinkellner

et al. 2010

Physiologia Plantarum

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“…In rice seedlings, the author observed bacteria colonizing the root and canopy tissue, especially in the secondary roots, root cortex, and leaf vascular tissue. Rhizobium penetration has also been observed in rice roots with the formation of large bacterial aggregates and between cells of the lateral root emergence region and root cortex (Webster et al, 1997).…”

Section: Discussionmentioning

confidence: 91%

Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice

Hahn

Sá

Osório

et al. 2016

Rev. Bras. Ciênc. Solo

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Rhizobia and associative bacteria promote growth in rice plants (Oryza sativa L.) through a series of mechanisms, but most studies on inoculation have been performed based on inoculation with these bacteria in a separate or singular manner. The objective of this study was to assess the efficiency of single/isolated inoculation and inoculation combined with symbiotic rhizobia from forage legume and with Azospirillum brasilense on promoting growth and the root colonization process in wetland rice. Two rhizobia among four isolates from a greenhouse and a laboratory experiment were selected that efficiently promoted seed germination and rice plant growth in a sterilized substrate and in soil. The two most efficient isolates (UFRGS Vp16 and UFRGS Lc348) were inoculated alone or in combination with a commercial product containing A. brasilense in two field experiments using two wetland rice cultivars over two growing seasons. In the field experiments, these isolates coinoculated with A. brasilense promoted larger increases in the agronomic variables of wetland rice compared to the control without inoculation. Confocal laser microscopy confirmed the presence of inoculated bacteria tagged with gfp (UFRGS Vp16, UFRGS Lc348, and A. brasilense) colonizing the root surface of the rice seedlings, mainly in the root hairs and lateral roots.

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“…Similarly, endophytic bacteria may penetrate into lateral root cortices by degrading root cell walls. The root junction site thus can serve as the starting point for both bacterial invasion of the primary root via the crack, which is caused by the outgrowth of the lateral root (Vasse et al 1995;Webster et al 1997Webster et al , 1998James et al 2002), and bacterial penetration into the elongation and differentiation zones of the newly emerged lateral root.…”

Section: Discussionmentioning

confidence: 99%

Constitutive expression of the nifA gene activates associative nitrogen fixation of Enterobacter gergoviae 57-7, an opportunistic endophytic diazotroph

Dong

Wang

et al. 2007

J Appl Microbiol

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Aims: This study was undertaken to investigate whether a nitrogen‐fixing bacterium Enterobacter gergoviae 57‐7, which was isolated from surface‐sterilized maize (Zea mays L.) roots, can colonize in roots and whether constitutive expression of the nifA gene encoding the transcriptional activator of nitrogenase genes can activate nif gene expression in planta. Methods and Results: Maize seedlings grown in an agar medium were inoculated with Ent. gergoviae strains containing the green fluorescent protein reporter gene. Root colonization and expression of the dinitrogenase reductase gene (nifH) by Ent. gergoviae were observed by confocal laser scanning microscopy. gfp‐tagged Ent. gergoviae was observed to colonize predominantly in cortical aerenchyma of primary and lateral roots and in stellar parenchyma cells and xylem vessels of primary roots. In planta nifH::gfp expression was not detected but after a constitutively expressed nifA gene was introduced into Ent. gergoviae. Conclusions: Enterobacter gergoviae 57‐7 is an opportunistic endophyte because it can live in soil and colonize in maize roots in the gnotobiotic agar culture. In agreement with previous 15N‐dilution evidence that Ent. gergoviae 57‐7 did not fix N2 in association with maize in pots whereas a derivative E7 containing a constitutively expressed nifA gene promoted plant growth partly through associative nitrogen fixation, constitutive expression of the nifA gene can activate bacterial nif gene expression in planta. Significance and Impact of the Study: This study and our previous studies suggest that manipulation of the promoter of the nifA gene in a nitrogen‐fixing bacterium having a high colonization competence is a practical and promising approach to achieve a stable associative nitrogen fixation for cereals.

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Interactions of rhizobia with rice and wheat

Cited by 31 publications

References 47 publications

The Rhizobium sp. strain NGR234 systemically suppresses arbuscular mycorrhizal root colonization in a split-root system of barley (Hordeum vulgare)

The Rhizobium sp. strain NGR234 systemically suppresses arbuscular mycorrhizal root colonization in a split-root system of barley (Hordeum vulgare)

Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice

Constitutive expression of the nifA gene activates associative nitrogen fixation of Enterobacter gergoviae 57-7, an opportunistic endophytic diazotroph

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