Interactions of Processes for Accumulation of Salt and Sugar in Barley Plants

Pitman, M. G.; Mowat, Julie; Nair, H.

doi:10.1071/bi9710619

Cited by 81 publications

(21 citation statements)

References 2 publications

(2 reference statements)

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“…The lower rates of respiration could be a consequence of a low supply of carbohydrates (23). The nature of the Cyt impairment at the whole leaf tissue level may be a consequence of a direct control of respiration by the turnover of ATP: a reduced ATP utilization in shoots of treated plants could explain the lower rate of respiration.…”

Section: Discussionmentioning

confidence: 99%

Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants

Jolivet¹,

Pireaux²,

Dizengremel³

1990

Plant Physiol.

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Treatment of barley (Hordeum vulgare) seedlings with 400 millimolar NaCI for 3 days resulted in a reduction in plant growth and an increase in the leaf content in ions (K+ + Na ) and proline. Purified mitochondria were successfully isolated from barley leaves. Good oxidative and phosphorylative properties were observed with malate as substrate. Malate-dependent electron transport was found to be only partly inhibited by cyanide, the remaining oxygen uptake being SHAM sensitive. The properties of mitochondria from NaCI-treated barley were modified. The efficiency of phosphorylation was diminished with only a slight decrease in the oxidation rates. In both isolated mitochondria and whole leaf tissue of treated plants, the lower respiration rate was due to a lower cytochrome pathway activity. In mitochondria, the activity of the alternative pathway was not modified by salt treatment, whereas this activity was increased in whole leaf tissue. The possible participation of the altemative pathway in response to salt stress will be discussed.Among crop plants, barley seems to be rather resistant to deleterious effects of NaCI salinity. However, under conditions of high saline environment barley plants do show growth reduction. In this context, changes in ion uptake (2), proline levels (33), gene expression (24), or more recently, changes in metabolic rates in roots (8) have been investigated. However, no work has been done on the role of mitochondria in leaves of barley plants submitted to high NaCl stress.The isolation and purification of green leaf mitochondria from barley was performed, and the oxidative and phosphorylative properties of these mitochondria were compared between control plant and the treated with 400 mM NaCl. The effects of salt on cyanide resistance were also investigated in both isolated mitochondria and whole leaf tissue to look for a role of the alternative pathway in salt-treated barley leaves. Uptake and compartmentation of ions in whole plant organs require energy mainly in the form of ATP generated from respiration (32). The production of compatible solutes is linked not only to the supply of reducing power and carbon skeletons, but also to energy production which depends on the efficiency of the phosphorylation process. Knowledge about respiratory metabolism during saline stress conditions is still lacking. In this context, the role of the nonphosphorylating cyanide-resistant pathway, which is a common feature of higher plant respiration (18, 28), is not fully elucidated. Some reports show that the activity ofthis alternative pathway could be modified in stress situations (17,25 MATERIALS AND METHODS Plant

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Section: Discussionmentioning

confidence: 99%

Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants

Jolivet¹,

Pireaux²,

Dizengremel³

1990

Plant Physiol.

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“…The samples of root were extracted with 80% (v/v) ethanol, as described above, and the extract used both for 14C determination and for chromatography. Solvents used were as described previously (Pitman, Mowat, and Nair 1971).…”

Section: (G) Tran8location Of 140-labelled Oompound8mentioning

confidence: 99%

Uptake and Transport of Ions in Barley Seedlings III. Correlation Between Transport to the Shoot and Relative Growth Rate

Pitman¹

1972

Aust. Jnl. Of Bio. Sci.

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128

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Determinations of potassium transport are described for plants growing at varied relative growth rates. These determinations were based on differences in total potassium content of the shoot over a 3.day period for plants growing on culture solution. It is shown that rate of transport from root to shoot is correlated with relative growth rate. The concentration of potassium in the shoot was independent of ratio of root to shoot, and little affected by relative growth rate (potassium was the only available univalent cation).Measurements of tracer uptake were used to estimate fluxes into the root and into the stele. Low rates of net uptake were due to low fluxes into the root as well as into the stele. At low rates of growth, rate of uptake was limited by the availability of energy substrates such as sugar. At higher growth rates sugar levels built up in the plant without corresponding increases in ion fluxes. The balance between growth and potassium uptake must have been achieved by other means in these conditions, such as translocation of growth substances.

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“…It is a major contributor to osmotic potential (Haschke and Liittge, 1975), although this particular function can be replaced to varying extents by other solutes (Pitman et al, 1971b;Kirkby and Mengel, 1976). It is responsible for stomatal opening (Humble and Hsiao, 1970) and many rapid movements of plant parts (Satter et al, 1974).…”

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confidence: 99%

Potassium Fluxes in Chlamydomonas reinhardtii (I.Kinetics and Electrical Potentials)

Malhotra

Glass

1995

Plant Physiol.

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Interactions of Processes for Accumulation of Salt and Sugar in Barley Plants

Cited by 81 publications

References 2 publications

Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants

Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants

Uptake and Transport of Ions in Barley Seedlings III. Correlation Between Transport to the Shoot and Relative Growth Rate

Potassium Fluxes in Chlamydomonas reinhardtii (I.Kinetics and Electrical Potentials)

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