Interactions of Porphyromonas gingivalis with epithelial cells

Duncan, Marilyn J.; Nakao, Shu; Skobe, Ziedonis; Xie, Hua

doi:10.1128/iai.61.5.2260-2265.1993

Cited by 179 publications

(126 citation statements)

References 16 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…The ¢mbrial adhesins are an integral part of the bacterial glycocalyx [1,11,12]. Most bacteria can display a number of adhesins: e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial adhesion to the oral mucosa has been demonstrated by in vitro studies [1] as well as by scanning electron microscopy (SEM) analysis of in vivo mucosal samples [2,3]. The molecular substrates of bacterial adhesion are adhesins located on the bacterial surface.…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria can easily be promoted to form ¢mbriae in vitro by addition of a high concentration of carbohydrates [9,10]. The occurrence of bacterial ¢mbriae in vitro has been visualised in transmission electron microscopy (TEM) by ruthenium red staining [1,6,11,12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fimbria-mediated bacterial adhesion to human oral epithelium

Vitkov

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

Oral mucosa biopsies and saliva samples from 12 individuals were processed for transmission (TEM) and scanning (SEM) electron microscopy with and without ruthenium red staining. Additionally performed microbiological estimations indicated in all bacteriological samples a facultative pathogenic flora. SEM and TEM investigation showed a diverse bacterial flora attached to the mucosal surface. Fimbriae comprising the glycocalyx and enabling bacterial attachment to the epithelial cells could be clearly visualised by ruthenium red. The only mode of bacterial attachment to the oral mucosa detected in the present investigation was fimbria-mediated adhesion and coadhesion. The fimbria-mediated adhesion enables the bacterial persistence in the oral cavity and is the first step in the bacterial colonisation process. ß

show abstract

“…The ¢mbrial adhesins are an integral part of the bacterial glycocalyx [1,11,12]. Most bacteria can display a number of adhesins: e.g.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fimbria-mediated bacterial adhesion to human oral epithelium

Vitkov

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…A number of studies have shown that P. gingivalis can adhere to and enter host cells, including epithelial (Duncan et al, 1993;Sandros et al, 1993;Lamont et al, 1995;Madianos et al, 1996;Rudney et al, 2001) and endothelial (Deshpande et al, 1998;Dorn et al, 2001;Yamatake et al, 2007) cells. However, following entry, it has not been clearly elucidated how P. gingivalis traffics within the infected cells.…”

Section: Extracellular Bacteriamentioning

confidence: 99%

Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway

Takeuchi

Furuta

Morisaki

et al. 2011

Cellular Microbiology

View full text Add to dashboard Cite

SummaryGingival epithelial cells function as an innate host defence system to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis, the most well-known periodontal pathogen, can enter gingival epithelial cells and pass through the epithelial barrier into deeper tissues. However, it is poorly understood how this pathogen exits from infected cells for further transcellular spreading. The present study was performed to elucidate the cellular machinery exploited by P. gingivalis to exit from immortalized human gingival epithelial cells. P. gingivalis was shown to be internalized with early endosomes positive for the FYVE domain of EEA1 and transferrin receptor, and about half of the intracellular bacteria were then sorted to lytic compartments, including autolysosomes and late endosomes/lysosomes, while a considerable number of the remaining organisms were sorted to Rab11-and RalA-positive recycling endosomes. Inhibition experiments revealed that bacterial exit was dependent on actin polymerization, lipid rafts and microtubule assembly. Dominant negative forms and RNAi knockdown of Rab11, RalA and exocyst complex subunits (Sec5, Sec6 and Exo84) significantly disturbed the exit of P. gingivalis. These results strongly suggest that the recycling pathway is exploited by intracellular P. gingivalis to exit from infected cells to neighbouring cells as a mechanism of cell-to-cell spreading.

show abstract

“…Before periodontopathogens can initiate a disease, they first have to colonize the oral cavity and surpass a certain threshold level. Porphyromonas gingivalis, together with Actinobacillus actinomycetemcomitans and Tannerella forsythensis (formerly Bacteroides forsythus), are major periodontopathogens that possess the ability to adhere 1,2 and invade [3][4][5] oral epithelium in vitro as well as in vivo. 6 Differences in the adhesion capacity of P. gingivalis have been found among both laboratory and clinical strains.…”

mentioning

confidence: 99%