Interactions of Polynucleotides and Other Polyelectrolytes with Enzymes and Other Proteins

Elbein, Alan D.

doi:10.1002/9780470122853.ch2

Advances in Enzymology - And Related Areas of Molecular Biology

1974

DOI: 10.1002/9780470122853.ch2

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Interactions of Polynucleotides and Other Polyelectrolytes with Enzymes and Other Proteins

Alan D. Elbein¹

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1977

1992

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Cited by 10 publications

References 133 publications

(40 reference statements)

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Effect of addition of polyethyleneimine on thermal stability and activity of glucose dehydrogenase

Teramoto

Nishibue

Okuhara

et al. 1992

Appl Microbiol Biotechnol

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Abstrzct. When polyethyleneimine (PEI), a water-soluble cationic polymer, was added to solutions of glucose dehydrogenase (GDH) from Bacillus megaterium IWG3 at a molar ratio of PEI to GDH greater than 10, the thermal stability of GDH markedly increased. By addition of PEI, the rate of GDH-catalysed oxidation of flo-glucose increased in a low concentration range of NAD + and NADP + and the Michaelis constants and inhibition constants for both NAD + and NADP + decreased. These results suggested that negatively charged GDH interacts with cationic water-soluble polymers to form conjugates by electrostatic attraction, and also that negatively charged coenzymes are "adsorbed" by the polymers, resulting in enrichment of the coenzymes in the vicinity of GDH. Addition of PEI was also found to be effective for preventing the denaturation of GDH by acrylamide.

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Effect of addition of polyethyleneimine on thermal stability and activity of glucose dehydrogenase

Teramoto

Nishibue

Okuhara

et al. 1992

Appl Microbiol Biotechnol

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The renal handling of polybasic drugs

Just

Habermann

1977

Naunyn-Schmiedeberg's Arch. Pharmacol.

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Adhärenz von Streptokokken der Gruppe A an Zellen des Mund-Rachen-Raumes: Lipoteicholsäure-Adhäsin und Fibronectin-Rezeptor

Beachey

Simpson

1982

Infection

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The attachment of group A streptococci to oropharyngeal epithelial cells is mediated by adhesive molecules (adhesins) on the surfaces of the micro-organisms that interact with receptor molecules on the epithelial cells. The evidence that the adhesin is composed of lipoteichoic acid (LTA) complexed with bacterial cell surface proteins is as follows: (a) Among the purified cell wall substances tested, only LTA was able to inhibit attachment; (b) treatment of streptococci with anti LTA but not with antibody against other surface substances blocks attachment; (c) LTa forms complexes with purified M protein, the most abundant protein on the surface of virulent streptococci; (d) the lipid moieties of LTA, which mediate attachment, remain free in the M protein-LTA complexes to interact with receptor analogues, such as serum albumin. The evidence that the receptor for the LTA mediated binding of streptococci resides in fibronectin molecules on oropharyngeal cells is as follows: (a) the addition ot adhesion test mixtures of fibronection inhibits binding; (b) the number of streptococci capable of attaching is directly proportional to the amount of fibronectin present on epithelial cells; (c) purified fibronectin immobilized on latex beads agglutinates suspensions of streptococci; (d) radiolabeled fibronectin binds to group A streptococci; (e) both the agglutination of fibronectin-beads and the binding of fibronectin to streptococci is blocked by LTA, the streptococcal adhesin.

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Interactions of Polynucleotides and Other Polyelectrolytes with Enzymes and Other Proteins

Cited by 10 publications

References 133 publications

Effect of addition of polyethyleneimine on thermal stability and activity of glucose dehydrogenase

Effect of addition of polyethyleneimine on thermal stability and activity of glucose dehydrogenase

The renal handling of polybasic drugs

Adhärenz von Streptokokken der Gruppe A an Zellen des Mund-Rachen-Raumes: Lipoteicholsäure-Adhäsin und Fibronectin-Rezeptor

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