Interactions of phosphorus-containing dendrimers with liposomes

Wrobel, Dominika; Йонов, Максим; Gardikis, Κonstantinos; Demetzos, Costas; Majoral, Jean‐Pierre; Pałecz, Bartłomiej; Klajnert, Barbara; Bryszewska, Maria

doi:10.1016/j.bbalip.2010.11.007

Cited by 42 publications

(46 citation statements)

References 37 publications

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“…The typical lipid concentration was 2.3 mM, and a 10:1 lipid: dendrimer molar ratio was used. For both types of liposomes, two heating/cooling cycles at a range of 5-45°C were performed at a 0.5°C/min scan rate [28,35].…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

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Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Wrobel

Appelhans

Signorelli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The influence of maltose-modified poly(propylene imine) (PPI) dendrimers on dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) (3%) liposomes was studied. Fourth generation (G4) PPI dendrimers with primary amino surface groups were partially (open shell glycodendrimers - OS) or completely (dense shell glycodendrimers - DS) modified with maltose residues. As a model membrane, two types of 100nm diameter liposomes were used to observe differences in the interactions between neutral DMPC and negatively charged DMPC/DMPG bilayers. Interactions were studied using fluorescence spectroscopy to evaluate the membrane fluidity of both the hydrophobic and hydrophilic parts of the lipid bilayer and using differential scanning calorimetry to investigate thermodynamic parameter changes. Pulsed-filed gradient NMR experiments were carried out to evaluate common diffusion coefficient of DMPG and DS PPI in D2O when using below critical micelle concentration of DMPG. Both OS and DS PPI G4 dendrimers show interactions with liposomes. Neutral DS dendrimers exhibit stronger changes in membrane fluidity compared to OS dendrimers. The bilayer structure seems more rigid in the case of anionic DMPC/DMPG liposomes in comparison to pure and neutral DMPC liposomes. Generally, interactions of dendrimers with anionic DMPC/DMPG and neutral DMPC liposomes were at the same level. Higher concentrations of positively charged OS dendrimers induced the aggregation process with negatively charged liposomes. For all types of experiments, the presence of NaCl decreased the strength of the interactions between glycodendrimers and liposomes. Based on NMR diffusion experiments we suggest that apart from electrostatic interactions for OS PPI hydrogen bonds play a major role in maltose-modified PPI dendrimer interactions with anionic and neutral model membranes where a contact surface is needed for undergoing multiple H-bond interactions between maltose shell of glycodendrimers and surface membrane of liposome.

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Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…After preparation, the suspension of liposomes was incubated in a water bath at 37°C for 10 min. For fluorescence experiments, the final lipid concentration was 100 μM, and the phospholipid/fluorescent probe molar ratio was 500:1 [28,29].…”

Section: Liposome Preparationmentioning

confidence: 99%

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Wrobel

Appelhans

Signorelli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Still there are only a few papers in which these methods are used in order to investigate the interactions between dendrimers and liposomes [34][35][36]. Klajnert et al used DSC to investigate the effect of hydrophilic and hydrophobic dendrimers on liposomes.…”

Section: Introductionmentioning

confidence: 99%

“…They found that the maximum percentage of PAMAM dendrimers that can incorporate into multilamellar liposomes is 5 mol% for G4 and 3.5 mol% for G3.5 [35]. Wrobel et al investigated the effect of positively charged phosphorous-containing dendrimers on unilamellar liposomes by measuring fluorescence anisotropy and DSC and showed that dendrimers interact with both hydrophobic and hydrophilic parts of the bilayer [36]. Smith and coworkers investigated the effect of G5 and G7 PAMAM dendrimers on multilamellar vesicles by solid-state NMR techniques.…”

Section: Introductionmentioning

confidence: 99%

A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers

Berényi

Mihály

Wacha

et al. 2014

Colloids and Surfaces B: Biointerfaces

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The effect of 5 th generation polyamidoamine (PAMAM G5) dendrimers on multilamellar dipalmitoylphosphocholine (DPPC) vesicles was investigated. PAMAM was added in two different concentration to the lipids (10 -3 and 10 -2 dendrimer/lipid molar ratios). The thermal behavior of the evolved systems was characterized by DSC; while the structure and the morphology were investigated with small-and wide-angel X-ray scattering (SWAXS), freeze-fracture electron microscopy (FFTEM) and phosphorus-31 nuclear magnetic resonance ( 31 P-NMR) spectroscopy, respectively. IR spectroscopy was used to study the molecular interactions between PAMAM and DPPC. The obtained results show that the dendrimers added in 10 -3 molar ratio to the lipids generate minor perturbations in the multilamellar structure and thermal character of liposomes, while added in 10 -2 molar ratio dendrimers cause major disturbance in the vesicular system. The terminal amino groups of the dendrimers are in strong interaction with the phosphate headgroups and through this binding dendrimers disrupt the regular multilamellar structure of DPPC. Besides highly swollen, fragmented bilayers, small vesicles are formed.

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“…These two peaks correspond to the known pre-transition and major transition temperature (T m ) of DMPC, respectively (Needham et al, 1988;Wrobel et al, 2011). Pre-transition is a low-enthalpy transition reflecting the membrane's change from the gel phase to the rippled gel phase, and the main phase transition is associated with the change of membranes from the rigid gel phase to the fluid liquid crystalline phase.…”

Section: Resultsmentioning

confidence: 99%

2-Hydroxyoleic acid-inserted liposomes as a multifunctional carrier of anticancer drugs

et al. 2017

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Studies have shown that insertion of oleic acid into lipid bilayers can modulate the membrane properties of liposomes so as to improve their function as drug carriers. Considering that 2-hydroxyoleic acid (2OHOA), a potential antitumor agent currently undergoing clinical trials, is a derivative of oleic acid, we explored the possibility of developing 2OHOA-inserted liposomes as a multifunctional carrier of antitumor drugs in the present study. The insertion of 2OHOA into lipid bilayers was confirmed by surface charge determination and differential scanning calorimetry. 2OHOA insertion greatly decreased the order of dimyristoylphosphatidylcholine packing, produced a nanosized (<100 nm) dispersion, and improved the colloidal stability of liposomes during storage. Moreover, 2OHOA-inserted liposome forms exhibited greater growth inhibitory activity against cancer cells compared with free 2OHOA, and the growth-inhibitory activity of liposomal 2OHOA was selective for tumor cells. 2OHOA insertion greatly increased the liposome-incorporated concentration of hydrophobic model drugs, including mitoxantrone, paclitaxel, and all-trans retinoic acid (ATRA). The in vitro anticancer activity of ATRAincorporated/2OHOA-inserted liposomes was significantly higher than that of ATRA-incorporated conventional liposomes. In a B16-F10 melanoma syngeneic mouse model, the tumor growth rate was significantly delayed in mice treated with ATRA-incorporated/2OHOA-inserted liposomes compared with that in the control group. Immunohistochemical analyses revealed that the enhanced antitumor activity of ATRA-incorporated/2OHOA-inserted liposomes was due, at least in part, to increased induction of apoptosis. Collectively, our findings indicate that 2OHOA-inserted liposomes exhibit multiple advantages as antitumor drug carriers, including the ability to simultaneously deliver two anticancer drugs -2OHOA and incorporated drug -to the tumor tissue.ARTICLE HISTORY

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Interactions of phosphorus-containing dendrimers with liposomes

Cited by 42 publications

References 37 publications

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers

2-Hydroxyoleic acid-inserted liposomes as a multifunctional carrier of anticancer drugs

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