Interactions of peroxynitrite and other nitrating substances with human platelets: the role of glutathione and peroxynitrite permeability

Lufrano, Maria; Balazy, Michael

doi:10.1016/s0006-2952(02)01584-8

Cited by 37 publications

(23 citation statements)

References 32 publications

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“…ONOO − can traverse a mean distance of 3, 5.5, and 0.5 μm in mitochondria, blood plasma, and erythrocytes, respectively, during one-half-life [9]. Lufrano and Balazy [20] have demonstrated that ONOO − is a diffusible molecule that may penetrate platelet membrane via a complex transport mechanism. It is also known that oxidative stress modulates platelet function and may lead to platelet aggregation [13].…”

Section: Discussionmentioning

confidence: 99%

Clovamide-rich extract from Trifolium pallidum reduces oxidative stress-induced damage to blood platelets and plasma

et al. 2011

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Numerous plants (including clovers) have been widely used in folk medicine for the treatment of different disorders. This in vitro study was designed to examine the antioxidative effects of the clovamide-rich fraction, obtained from aerial parts of Trifolium pallidum, in the protection of blood platelets and plasma against the nitrative and oxidative damage, caused by peroxynitrite (ONOO(-)). Carbonyl groups and 3-nitrotyrosine in blood platelet and plasma proteins were determined by ELISA tests. Thiol groups level was estimated by using 5,5'-dithio-bis(2-nitro-benzoic acid, DTNB). Plasma lipid peroxidation was measured spectrophotometrically as the production of thiobarbituric acid reactive substances. The results from our work indicate that clovamide-rich T. pallidum extract may reveal the protective properties in the prevention against oxidative stress. The presence of clovamide-rich T. pallidum extract (12.5-100 μg/ml) partly inhibited ONOO(-)-mediated protein carbonylation and nitration. All the used concentrations of T. pallidum extract reduced lipid peroxidation in plasma. The antioxidative action of the tested extract in the protection of blood platelet lipids was less effective; the extract at the lowest final concentration (12.5 μg/ml) had no protective effect against lipid peroxidation. The present results indicate that the extract from T. pallidum is likely to be a source of compounds with the antioxidative properties, useful in the prevention against the oxidative stress-related diseases.

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Section: Discussionmentioning

confidence: 99%

Clovamide-rich extract from Trifolium pallidum reduces oxidative stress-induced damage to blood platelets and plasma

et al. 2011

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“…O À 2 reacts rapidly with • NO, forming ONOO − , a strong physiological oxidant species. In blood platelets, peroxynitrite may be also formed (Lufrano and Balazy 2003). Proteins are the main targets of ONOO − action on blood platelets.…”

Section: Discussionmentioning

confidence: 99%

l-carnitine modulates blood platelet oxidative stress

et al. 2010

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The oxidative stress induced by acute exertion may interfere with blood platelet activation. The beneficial effect of L-carnitine (gamma-trimethylamino-beta-hydroxybutyric acid) on oxidative stress in blood platelets has not been fully investigated; however, different studies indicate that this compound modulates platelet functions. The aim of our study was to assess the effects of L-carnitine on platelet activation and oxidative/nitrative protein damage (determined by the levels of protein carbonyl groups, thiol groups, and 3-nitrotyrosine residues) in resting blood platelets or platelets treated with peroxynitrite (ONOO(-), a strong physiological oxidant) in vitro. We also investigated the effects of L-carnitine on the level of platelet glutathione and on the formation of superoxide anion radicals O2(-*), lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) in blood platelets stimulated by thrombin (a strong physiological agonist), and platelet aggregation induced by adenosine diphosphate (a strong physiological stimulator). We have observed that carnitine decreases platelet activation (measured by platelet aggregation, the generation of O2(-*), and TBARS production). Moreover, our results in vitro demonstrate that carnitine may protect against oxidation of thiol groups induced by ONOO(-). Thus, carnitine may have some protectory effects against oxidative changes induced in blood platelets.

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“…Great amount of ONOO − are generated during inflammation. Various tests showed that in blood platelets peroxynitrite modulates blood platelet functions [2,3] and is an important contributor to cardiovascular diseases and inflammatory conditions, including arthritis, endotoxic and septic shock [35]. Earlier we have found that ONOO − could induce a damage in some biological molecules in platelets, such as proteins and lipids [4].…”

Section: Discussionmentioning

confidence: 99%

The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins

Saluk-Juszczak

Pawlaczyk

Olas

et al. 2010

International Journal of Biological Macromolecules

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Interactions of peroxynitrite and other nitrating substances with human platelets: the role of glutathione and peroxynitrite permeability

Cited by 37 publications

References 32 publications

Clovamide-rich extract from Trifolium pallidum reduces oxidative stress-induced damage to blood platelets and plasma

Clovamide-rich extract from Trifolium pallidum reduces oxidative stress-induced damage to blood platelets and plasma

l-carnitine modulates blood platelet oxidative stress

The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins

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