Interactions of Nocardia asteroides with murine glia cells in culture

Beaman, L; Beaman, Blaine L.

doi:10.1128/iai.61.1.343-347.1993

Cited by 19 publications

(13 citation statements)

References 9 publications

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“…This strain was originally isolated from the soil, and it has been designated the working type reference strain for the N. asteroides taxon (sensu stricto) (2). Strain 19247 shares many morphologic, structural, physiologic, and biochemical features with GUH-2, yet it is nonpathogenic for mice and does not invade cells (4,14,15). This strain has been utilized extensively as a negative control for studies on mechanisms of nocardial virulence (4,19).…”

Section: Methodsmentioning

confidence: 99%

“…Log-phase cells of the model neuroinvasive strain N. asteroides GUH-2 invade both pulmonary epithelial cells and capillary endothelial cells. This organism also penetrates the surface of and becomes internalized in primary cultures of neonatal murine type II but not type I astroglia cells (14), the artery endothelial cell line CPAE, and human astrocytoma cell lines (15). Pretreatment with a microfilament inhibitor, cytochalasin, significantly reduces internalization of the nocardiae in some, but not all, cell lines.…”

mentioning

confidence: 99%

“…Log-phase and stationary-phase cells of N. asteroides GUH-2 bind longitudinally to the surfaces of host cells (14,15), and both are readily internalized by phagocytic cells. However, only filamentous cells of GUH-2 attach by way of the tip, resulting in penetration and invasion of nonphagocytic cells (4,10,14,15). All of these observations suggest multiple mechanisms for nocardial adherence to and internalization in host cells.…”

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confidence: 99%

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Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

Beaman¹,

Beaman

1998

Infect Immun

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The interactions of Nocardia asteroides GUH-2 with pulmonary epithelial cells of C57BL/6 mice and with HeLa cells were studied. Electron microscopy demonstrated that only the tips of log-phase cells penetrated pulmonary epithelial cells following intranasal administration, and nocardiae were recovered from the brain. Coccobacillary cells neither invaded nor disseminated. Serum from immunized mice (IMS) decreased attachment to and penetration of pulmonary epithelial cell surfaces by log-phase GUH-2 and inhibited spread to the brain. IMS was adsorbed against stationary-phase cells. Western immunoblots suggested that this adsorbed IMS was reactive primarily with 43- and 62-kDa proteins. Immunofluorescence showed that adsorbed IMS preferentially labeled the tips of log-phase GUH-2 cells. Since this IMS was reactive to culture filtrate antigens, several of these proteins were cut from gels, and mice were immunized. Sera against 62-, 55-, 43-, 36-, 31-, and 25-kDa antigens were obtained. The antisera against the 43- and 36-kDa proteins labeled the filament tips of GUH-2 cells. Only the antiserum against the 43-kDa antigen increased pulmonary clearance, inhibited apical attachment to and penetration of pulmonary epithelial cells, and prevented spread to the brain. An in vitro model with HeLa cells demonstrated that the tips of log-phase cells of GUH-2 adhered to and penetrated the surface of HeLa cells. Invasion assays with amikacin treatment demonstrated that nocardiae were internalized. Adsorbed IMS blocked attachment to and invasion of these cells. These data suggested that a filament tip-associated 43-kDa protein was involved in attachment to and invasion of pulmonary epithelial cells and HeLa cells by N. asteroides GUH-2.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

Beaman¹,

Beaman

1998

Infect Immun

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“…Small numbers of compact, phagocytic cells were scattered throughout the brain. These were probably microglia, which are macrophage-equivalent cells within the brain (9). The microglia engulfed areas of tissue that contained the invading nocardial cells, and nocardiae were observed within these more distinctive cells.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the nocardiae appeared to be growing in some of the microglia (data not shown). Nevertheless, it seems likely that microglia were critical to preventing continued growth of nocardiae in the brain during a sublethal infection (9,16). Furthermore, microglia probably represented the most important means by which the nocardiae were eliminated from the brain 10 to 14 days after infection, because it was shown that microglia cultured in vitro for 14 days were able to prevent nocardial growth whereas in vitro-grown astroglia were not (9).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural analysis of growth of Nocardia asteroides during invasion of the murine brain

Beaman

1993

Infect Immun

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BALB/c mice were infected with 106 CFU of log-phase cells of Nocardia asteroides GUH-2 by tail vein injection (at this lethal inoculum dose, approximately 800 to 1,000 CFU becomes deposited in the brain). At 24 h after infection, the ultrastructural interactions of the nocardiae during growth within the murine brain were investigated. The nocardiae grew perivascularly in the pons, substantia nigra, hypothalamus, and thalamus portions of the brain, where they were either within or associated with most brain cell types. There appeared to be a propensity for growth within the soma of neurons and their axonal extensions. The nocardial cells were surrounded by 1 to 30 layers of membrane, and the innermost membrane was usually tightly adherent to the cell wall. This compartmentalization of nocardiae within brain cells could contribute to their failure to induce an inflammatory response or a cytopathic effect. Nevertheless, the bacteria were able to obtain adequate nutrients from the host to grow within the brain. The nocardiae were not completely inert, since some of the brain cells showed signs of degeneration. The myelin sheaths of axons were the most strongly affected, and there was evidence of demyelinization and axonal degeneration. Nocardiae growing within brain cells were

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Neuroinvasive Nocardia asteroides GUH-2 Induces Apoptosis in the Substantia Nigra in Vivo and Dopaminergic Cells in Vitro

Tam¹,

Barry²,

Beaman³

et al. 2002

Experimental Neurology

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Interactions of Nocardia asteroides with murine glia cells in culture

Cited by 19 publications

References 9 publications

Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

Ultrastructural analysis of growth of Nocardia asteroides during invasion of the murine brain

Neuroinvasive Nocardia asteroides GUH-2 Induces Apoptosis in the Substantia Nigra in Vivo and Dopaminergic Cells in Vitro

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