Interactions of lectins and their saccharide receptors in theRhizobium-legume symbiosis

Dazzo, Frank B.; Truchet, G.

doi:10.1007/bf01870336

Cited by 87 publications

(55 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After research (Hamblin and Kent, 1973) had shown that bean lectin could bind to a microsymbiont, the Rhizobium-Leguminosae symbiosis was thoroughly studied (Dazzo and Truchet, 1983;Kijne et al, 1986); in some legumes, for example, soybean (Halverson and Stacey, 1985), it seems that a lectin synthesized by the plant may play a role in recognition.…”

mentioning

confidence: 99%

Characterization of a Lectin from Lactarius deterrimus (Research on the Possible Involvement of the Fungal Lectin in Recognition between Mushroom and Spruce during the Early Stages of Mycorrhizae Formation)

Giollant¹,

Guillot²,

Damez³

et al. 1993

Plant Physiol.

View full text Add to dashboard Cite

A lectin (LDetL) was isolated from carpophores of the mushroom Lactarius deterrimus, a specific symbiont of the spruce, by a combination of affinity, hydroxylapatite, and gel-filtration chromatography. Its molecular mass, as determined by gel filtration, is about 37,000 D, and its structure is dimeric, with two identical subunits assembled by noncovalent bonds. It appeared homogeneous on high-performance liquid chromatography gel filtration, but isoelectric focusing revealed microheterogeneity, with a main band in the pH zone near 6.5. Amino acid analysis showed that LDetL contains a large proportion of glycine and especially methionine. Hapten inhibition assay indicated that LDetL is most specific for ~-~-g a l a c t o s y l ( l~3 ) -~-N -a c e t y lgalactosamine residues. The lectin was formed in the in vitro-cultivated mycelium, and anti-lectin antibodies revealed by indirect immunofluorescence the presence of lectin in the cell wall. Receptor sites for LDetL were found on the roots, especially on the root hairs, of axenically grown spruce seedlings. The lectin LDL previously isolated by us from the taxonomically related mushroom Lactarius deliciosus, a symbiont of the pine, does not bind to the spruce radicle. This suggests a role of the funga1 lectin in recognition and specificity during the early stages of mycorrhizae formation.

show abstract

mentioning

confidence: 99%

Characterization of a Lectin from Lactarius deterrimus (Research on the Possible Involvement of the Fungal Lectin in Recognition between Mushroom and Spruce during the Early Stages of Mycorrhizae Formation)

Giollant¹,

Guillot²,

Damez³

et al. 1993

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Considerable attention has been devoted to the mechanisms by which a Rhizobiqm strain and its host plant might recognize each other and by which the bacteria enter the nodules which they induce. It has been hypothesized that surface or extracellular polysaccharides produced by the bacterial symbiont are involved in these processes (2,10). Mutants that do not produce an acidic exopolysaccharide have been described for several strains of Rhizobium (5-8, 13, 17, 28, 35), and in most cases such mutants form empty, non-nitrogen-fixing nodules (6-8, 13, 17, 28, 35).…”

mentioning

confidence: 99%

Genetic analysis of a cluster of genes required for synthesis of the calcofluor-binding exopolysaccharide of Rhizobium meliloti

et al. 1988

View full text Add to dashboard Cite

Rhizobium melioti produces an acidic, Calcofluor-binding exopolysaccharide which plays a role in nodulation of alfalfa plants by this bacterium. We constructed and mapped 102 transposon insertions in a 48-kilobase (kb) region previously shown to contain several exo genes. Mutations affecting production of the Calcofluor-binding exopolysaccharide were clustered in a 22-kb region and fell into 12 complementation groups. Strains carrying mutations in seven of the complementation groups (exoA, exoB, exoF, exoL, exoM, exoP, and exoQ) produced no Calcofluor-binding exopolysaccharide and induced non-nitrogen-fixing nodules on alfalfa. Mutants in an eighth complementation group, exoH (Leigh et al., Cell 51:579-587, 1987), produce an altered exopolysaccharide and also induce the formation of non-nitrogen-fixing nodules. Mutants in the remaining four complementation groups produced less Calcofluor-binding material than the wild type. Mutants carrying mutations in two of these complementation groups (exoK and exoN) formed apparently normal, nitrogen-fixing nodules, while mutants in the other two groups (exoG and exoJ) formed normal nodules less efficiently than the wild type.Rhizobia fix nitrogen in symbiotic association with leguminous plants. In the course of this association, the bacteria induce the formation of nodules on the roots of the plant, enter these nodules through tubes called infection threads, and differentiate and begin to fix nitrogen once inside (for reviews see references 2, 20, 30, and 44). Considerable attention has been devoted to the mechanisms by which a Rhizobiqm strain and its host plant might recognize each other and by which the bacteria enter the nodules which they induce. It has been hypothesized that surface or extracellular polysaccharides produced by the bacterial symbiont are involved in these processes (2, 10). Mutants that do not produce an acidic exopolysaccharide have been described for several strains of 13,17,28,35), and in most cases such mutants form empty, non-nitrogen-fixing nodules (6-8, 13, 17, 28, 35). Exopolysaccharide-deficient mutants that fail to nodulate or that are nodulation and fixation proficient have also been described (5, 38).We have previously described TnS insertion mutants of Rhizobium meliloti RmlO21 that do not produce a Calcofluor-binding, acidic exopolysaccharide (17, 28). These exo mutants fail to invade the nodules they induce on alfalfa, which are consequently Fix-(do not fix nitrogen). All of the nod genes are required for nodule induction by these mutants (26 (18,23). Plasmids that complement these mutations have been isolated from a library of R. meliloti DNA (28). In this paper we describe the mapping of the region covered by these plasmids and the isolation of more TnS insertion mutations in this region. The number of loci in the cluster affecting exopolysaccharide biosynthesis now stands at 12. MATERIALS AND METHODSStrains, plasmids, and media. Bacterial strains and plasmids are listed in Table 1. Bacteria were grown in LB medium (32), with 2.5 mM M...

show abstract

“…Othe have described lectin release from roots and see 24), but in none of these studies was the speci release examined. The 10 mM NaAn analogy can be observed between the localization pattern isphate (ph 7.2); of DSA and that of other N-acetylglucosamine-oligomer-specific buffer contained lectins. Indeed, the tissue distribution of WGA in wheat grains, I seeds were in-resembles that of DSA in that it is also almost exclusively reoropriate buffers.…”

Section: Discussionmentioning

confidence: 66%

“…Although carbohydrate-binding proteins are now widely used as biochemical tools in a growing number of applications (22), little is known about their physiological function in the plant. It has often been suggested that legume lectins are involved in recognition processes between nitrogen-fixing Rhizobia and legume roots (3,10). Other investigators have postulated that seed lectins may protect young seedlings against pathogens (16,23,25).…”

mentioning

confidence: 99%

Datura stramonium Agglutinin

Broekaert

Lambrechts²,

Verbelen³

et al. 1988

Plant Physiol.

View full text Add to dashboard Cite

The distribution of Datura stramonium agglutinin over different tissues of D. stramonium L. seeds was visualized by immunocytochemical techniques and quantified by agglutination assays. The lectin occurs predominantly in the outer seed tissues (seed coat and seed epidermis), where it is associated, at least in part, with the cell wails. Developing D. stramonium seeds secrete newly synthesized lectin polypeptides into the incubation medium, which confirms the extraceOular location of the lectin. Imbibition of mature decoated seeds results in a rapid and highly specific release of lectin. Indeed, imbibition solutions contain almost exclusively the lectin together with a few other carbohydrate-binding proteins; this is indicative of the predominance of these proteins in the seed surface layer. The presence of important amounts of lectin in the outer tissues of the seed is consistent with a possible role in the mediation of cell-cell interactions.

show abstract

Interactions of lectins and their saccharide receptors in theRhizobium-legume symbiosis

Cited by 87 publications

References 91 publications

Characterization of a Lectin from Lactarius deterrimus (Research on the Possible Involvement of the Fungal Lectin in Recognition between Mushroom and Spruce during the Early Stages of Mycorrhizae Formation)

Characterization of a Lectin from Lactarius deterrimus (Research on the Possible Involvement of the Fungal Lectin in Recognition between Mushroom and Spruce during the Early Stages of Mycorrhizae Formation)

Genetic analysis of a cluster of genes required for synthesis of the calcofluor-binding exopolysaccharide of Rhizobium meliloti

Datura stramonium Agglutinin

Contact Info

Product

Resources

About