Interactions of lead(II) with nucleotides and their constituents

Sigel, Helmut; Costa, Carla P. Da; Martin, R. Bruce

doi:10.1016/s0010-8545(01)00343-5

Cited by 79 publications

(78 citation statements)

References 55 publications

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“…For Pb 2 + , the 10-membered chelate bridging two neighboring phosphate residues is an important species, and this is most likely [26] also true for Ca 2 + . Zn 2 + can form both types of isomers, with a preference for the N7 one [Eq.…”

Section: Discussionmentioning

confidence: 99%

“…[22,25,26] Evidently, the most frequently repeated individual site is the phosphate diester bridge, -O-P(O) 2 À -O-, in which the two terminal oxygen atoms together carry one negative charge. It is difficult to measure the metal ion affinity of such a site in a polymer or even in an oligonucleotide such as UpUpU 2À directly as the primary protons of phosphate residues are released with pK a values of about 1 (or below), [17] and thus no competition for binding occurs between a metal ion and a proton within the experimentally accessible pH range, which includes the physiological one.…”

Section: H T U N G T R E N N U N G Tonated Ma C H T U N G T R E N N Umentioning

confidence: 99%

“…Because any kind of chelate formation must lead to an enhanced complex stability, [26][27][28]35] it is necessary to determine the stability of the open isomer in Equilibrium (14). This stability constant cannot be measured directly, although estimates can be made based on the stability of MA C H T U N G T R E N N U N G (dGuo) 2 + complexes.…”

Section: Solution Structures Of the Ma C H T U N G T R E N N U N G [Hmentioning

confidence: 99%

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Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Knobloch

Sigel

Okruszek

et al. 2007

Chemistry A European J

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The interaction between divalent metal ions and nucleic acids is well known, yet knowledge about the strength of binding of labile metal ions at the various sites is very scarce. We have therefore studied the stabilities of complexes formed between the nucleic acid model d(pGpG) and the essential metal ions Mg2+ and Zn2+ as well as with the generally toxic ions Cd2+ and Pb2+ by potentiometric pH titrations; all four ions are of relevance in ribozyme chemistry. A comparison of the present results with earlier data obtained for M(pUpU)- complexes allows the conclusion that phosphate-bound Mg2+ and Cd2+ form macrochelates by interaction with N7, whereas the also phosphate-coordinated Pb2+ forms a 10-membered chelate with the neighboring phosphate diester bridge. Zn2+ forms both types of chelates with formation degrees of about 91% and 2.4% for Zn[d(pGpG)]cl/N7 and Zn[d(pGpG)]-cl/PO, respectively; the open form with Zn2+ bound only to the terminal phosphate group, Zn[d(pGpG)]-op, amounts to about 6.8 %. The various intramolecular equilibria have also been quantified for the other metal ions. Zn2+, Cu2+, and Cd2+ also form macrochelates in the monoprotonated M[H;d(pGpG)] species (the proton being at the terminal phosphate group), that is, the metal ion at N7 interacts to some extent with the P(O)2(OH)- group. Thus, this study demonstrates that the coordinating properties of the various metal ions toward a pGpG unit in a nucleic acid differ: Mg2+, Zn2+, and Cd2+ have a significant tendency to bridge the distance between N7 and the phosphate group of a (d)GMP unit, although to various extents, whereas Pb2+ (and possibly Ca2+) prefer a pure phosphate coordination.

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Section: Discussionmentioning

confidence: 99%

Section: H T U N G T R E N N U N G Tonated Ma C H T U N G T R E N N Umentioning

confidence: 99%

Section: Solution Structures Of the Ma C H T U N G T R E N N U N G [Hmentioning

confidence: 99%

See 1 more Smart Citation

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Knobloch

Sigel

Okruszek

et al. 2007

Chemistry A European J

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“…In spite of these difficulties, the multitude of methods summarized in this chapter enable us to ) of a water molecule in the aqua complex, the ligand exchange rate from the first coordination sphere of the metal ion (k exch ), the logarithm of the affinity towards acetate (log K O ), the affinity towards NH 3 (log K N ), the affinity towards the phosphate group in AMP (log  M(AMPS) ) (see also Figure 2 [161,247] log K AMPS,calc 1.24 ± 0.08 0.97 ± 0.07 1.22 ± 0.06 1.96 ± 0.11 1.67 ± 0.12 1.74 ± 0.09 2.27 ± 0.20 4.32 ± 0.14 4.79 ± 0.15 [161,248] log  M(AMPS) -0.01 ± 0.10 -0.06 ± 0.08 -0.03 ± 0.07 0.16 ± 0.12 0.07 ± 0.14 0.16 ± 0.10 0.66 ± 0.21 2.37 ± 0.15 2.55 ± 0.13 [161,248] a The numbers in brackets refer to CN = 8. High-spin electron configuration.…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

Methods to Detect and Characterize Metal Ion Binding Sites in RNA

Erat

Sigel

2011

Structural and Catalytic Roles of Metal Ions in RNA

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Metal ions are inextricably associated with RNAs of any size and control their folding and activity to a large part. In order to understand RNA mechanisms, also the positioning, affinities and kinetics of metal ion binding must be known. Due to the spectroscopic silence and relatively fast exchange rates of the metal ions usually associated with RNAs, this task is extremely challenging and thus numerous methods have been developed and applied in the past. Here we provide an overview on the different metal ions and methods applied in RNA (bio)chemistry: The physical-chemical properties of important metal ions are presented and briefly discussed with respect to their application together with RNA. Each method ranging from spectroscopic over biochemical to computational approaches is briefly described also mentioning caveats that might occur during the experiment and/or interpretation of the results

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“…1,2 On the other hand, since it is slowly eliminated, lead accumulates in liver, kidneys, bones, and other parts of the body. 3 So, the design of drugs to counteract the effects of lead poisoning requires establishing the preferred ligands of Pb(II) and their stereochemistry. Consequently, synthesis of antidotes for lead(II) ion toxicosis has attracted more and more attention, and numerous reports concerning experimental coordination chemistry of lead(II) have been published.…”

Section: Introductionmentioning

confidence: 99%

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C₅H₄NCOO)₂]

2008

Bulletin of the Korean Chemical Society

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The title compound of nicotinato lead(II) complex [Pb(C 5 H 4 NCOO) 2 ] has been optimized at B3LYP/ LANL2DZ and HF/LANL2DZ levels of theory. The calculated results show that the lead(II) ion adopts 2-coordinate geometry, which is the same as its crystal structure and different from the 4-coordinate geometry of isonicotinato lead(II) complex. Atomic charge distributions indicate that during forming the title compound, each nicotinic acid ion transfers their negative charges to central lead(II) ion. The electronic spectra calculated by B3LYP/LANL2DZ level show that there exist two absorption bands, which have some red shifts compared with those of isonicotinato lead(II) complex and the electronic transitions are mainly derived from intraligand π-π * transition and ligand-to-metal charge transfer (LMCT) transition. CIS-HF method is not suitable for the system studied here. The thermodynamic properties of the title compound at different temperatures have been calculated and corresponding relations between the properties and temperature have also been obtained. The second order optical nonlinearity was calculated, and the molecular hyperpolarizability was 1.147754 × 10 -30 esu.

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Interactions of lead(II) with nucleotides and their constituents

Cited by 79 publications

References 55 publications

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Methods to Detect and Characterize Metal Ion Binding Sites in RNA

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C₅H₄NCOO)₂]

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Interactions of lead(II) with nucleotides and their constituents

Cited by 79 publications

References 55 publications

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)3−): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)3−): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Methods to Detect and Characterize Metal Ion Binding Sites in RNA

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C5H4NCOO)2]

Contact Info

Product

Resources

About

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Metal‐Ion‐Coordinating Properties of the Dinucleotide 2′‐Deoxyguanylyl(5′→3′)‐2′‐deoxy‐5′‐guanylate (d(pGpG)³⁻): Isomeric Equilibria Including Macrochelated Complexes Relevant for Nucleic Acids

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C₅H₄NCOO)₂]