Interactions of lamotrigine with single- and double-stranded DNA under physiological conditions

Morawska, Kamila; Popławski, Tomasz; Ciesielski, Witold; Smarzewska, Sylwia

doi:10.1016/j.bioelechem.2020.107630

Cited by 13 publications

(7 citation statements)

References 58 publications

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“…Therefore, considering the fact that the main aim of the present work was DNA interaction studies, further measurements were performed in phosphate buffered saline at physiological pH and acetate buffer at pH 4.7. These buffers are commonly used for interaction studies 3 , 15 , 29 .…”

Section: Resultsmentioning

confidence: 99%

Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA

Smarzewska,

Ignaczak,

Koszelska

2024

Sci Rep

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In this study, electrochemical and theoretical studies were performed to explain the interaction mechanism between ponatinib (PNT), a third generation tyrosine kinase inhibitor, and dsDNA. The electrochemical part was conducted in phosphate-buffered saline (PBS) at physiological pH of 7.4 and in acetate buffer with a pH of 4.7, using square wave voltammetry. A boron-doped diamond electrode was used in a bulk-incubated solution. The theoretical part was investigated using computational methods, such as the semiempirical method PM7 and density functional theory (DFT). Significant differences in the electrochemical behavior of PNT in the presence of DNA confirmed the occurrence of interactions. The results obtained in the acetate buffer strongly suggested the preferential interaction of PNT with guanine residues. However, at physiological pH, it can be concluded that PNT interacts with dGua and dAdo in the dsDNA molecule. These results are consistent with outcomes from the theoretical studies, where quantum-chemical calculations showed that both electrochemically detectable nucleobases form hydrogen bonds with the drug. These bonds appeared to be stronger with guanine than with adenine. According to the computational studies, the dsDNA major groove is the energetically preferred site for the complexation of PNT.

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Section: Resultsmentioning

confidence: 99%

Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA

Smarzewska,

Ignaczak,

Koszelska

2024

Sci Rep

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“…The binding constant of this equilibrium can be calculated using voltammetric data based on the literature equations given: indicates the binding ratio [31][32][33]. Because Eq.…”

Section: (Dsdna)-prop Interaction Studiesmentioning

confidence: 99%

A new screen-printed carbon sensor decorated gold nanoparticles/kaolinite mineral: electrochemical analysis of Propyphenazone and the investigation of ds-DNA interaction

Yıldız,

Bayraktepe,

Yazan

2023

Preprint

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This work represents the development of natural kaolinite mineral (Kao)−gold nanoparticles (GNPs) modified screen-printed carbon electrode (SPCE) based on biocompatible electrode material for the electrochemical quantification of Propyphenazone (PROP) and the investigation of the interaction between PROP and ds-DNA. The surface characteristics of the Kao−GNPs/SPCE were examined by Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS), and Field-Emission Scanning Electron Microscopy (FE-SEM) with Energy Dispersive X-ray Spectroscopy (EDS) methods. The effect of Kao and gold compositions, pH, and interferences studied the analytical performance of this sensor. PROP has an irreversible oxidation signal on Kao−GNPs/SPCE sensor surface. Based on this oxidation signal, calibration works were carried out using differential pulse voltammetry (DPV), and linear working range, LOD, and LOQ were determined. The real sample analysis of PROP was implemented in human serum samples with a recovery value of 99.43%. Binding constant (K) and Gibbs free energy change (ΔG°) relating to the interaction between PROP and ds-DNA were calculated to be 2.14 (± 0.42) × 104 M− 1 and − 24.70 kJ mol− 1, respectively.

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“…Due to its incomplete metabolization and deconjugation, it has been detected in wastewater and wastewater‐contaminated freshwater 22,23 . It is potentially hazardous to aquatic fauna and harmful to human life 24 . We chose these two pharmaceuticals as the target pollutants because studies suggest that their treatment by conventional methods is inadequate 25,26 .…”

Section: Introductionmentioning

confidence: 99%

“…22,23 It is potentially hazardous to aquatic fauna and harmful to human life. 24 We chose these two pharmaceuticals as the target pollutants because studies suggest that their treatment by conventional methods is inadequate. 25,26 Further, there are very limited studies on electrochemical treatments of MET and LAM.…”

Section: Introductionmentioning

confidence: 99%

Apparent and true mineralization during combined electro‐oxidation and electro‐coagulation on stainless steel anode

Meena

Ghatak

2023

Asia-Pacific J Chem Eng

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Present study is a novel attempt to experimentally verify that electrochemical treatment of organic pollutants on stainless steel (SS) anode is a combination of electro‐oxidation (EO) and electro‐coagulation (EC) with quantitative estimation of individual contributions of the two mechanisms. Target organic pollutants, metformin HCl (MET‐HCl) and lamotrigine (LAM), were electrochemically treated with SS anode for this purpose. Experiments were designed using central composite rotatable design (CCRD) to assess the effect of current density (CD) and supporting electrolyte concentration (SEC) as independent process parameters. Under all conditions, the process was experimentally verified to be a combination of EO and EC. For MET‐HCl, true mineralization (TM) as a result of EO contribution varied between 50.13% and 66.64%. EO contribution and resulting TM for LAM ranged from 37.5% to 61.83%. EC contributed 30.6%–31.84% towards MET‐HCl remediation causing the TOC to be transferred from the liquid to the sludge produced (SP). EC contribution was 23.68%–35.89% for LAM remediation. Thus, apparent mineralization (AM), the sum of EO and EC contributions, ranged from 80.63% to 97.79% for MET‐HCl and 61.18% to 96.82% for LAM.Process parameters were statistically optimized using response surface methodology (RSM) to simultaneously maximize TM and AM with minimal energy consumption and maximal current efficiency. The optimized conditions were 0.83 mA/cm2 CD and 86.66 ppm SEC for MET‐HCl. The corresponding values were 1.31 mA/cm2 CD and 79.51 ppm SEC for LAM.

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Interactions of lamotrigine with single- and double-stranded DNA under physiological conditions

Cited by 13 publications

References 58 publications

Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA

Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA

A new screen-printed carbon sensor decorated gold nanoparticles/kaolinite mineral: electrochemical analysis of Propyphenazone and the investigation of ds-DNA interaction

Apparent and true mineralization during combined electro‐oxidation and electro‐coagulation on stainless steel anode

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