Interactions of Isoprenoid Pathway Enzymes and Indirect Stimulation of Isoprenoid Biosynthesis by Pentose Phosphate Cycle Substrates in Synechocystis PCC 6803

Poliquin, Kelly; Cunningham, Francis X.; Gantt, Raymond; Gantt, Elisabeth

doi:10.1007/978-1-4614-4063-5_4

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…However, a recent finding indicates that while addition of various PPC compounds supported in vitro terpenoids synthesis, the PPC compounds did not directly serve as substrates. From these results, the authors concluded that the in vitro stimulation by PPC compounds is indirect and does not occur via the MEP pathway [ 52 ].…”

Section: Terpenoids Produced In Cyanobacteriamentioning

confidence: 99%

Terpenoids and Their Biosynthesis in Cyanobacteria

Pattanaik

Lindberg

2015

Life

145

103

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Terpenoids, or isoprenoids, are a family of compounds with great structural diversity which are essential for all living organisms. In cyanobacteria, they are synthesized from the methylerythritol-phosphate (MEP) pathway, using glyceraldehyde 3-phosphate and pyruvate produced by photosynthesis as substrates. The products of the MEP pathway are the isomeric five-carbon compounds isopentenyl diphosphate and dimethylallyl diphosphate, which in turn form the basic building blocks for formation of all terpenoids. Many terpenoid compounds have useful properties and are of interest in the fields of pharmaceuticals and nutrition, and even potentially as future biofuels. The MEP pathway, its function and regulation, and the subsequent formation of terpenoids have not been fully elucidated in cyanobacteria, despite its relevance for biotechnological applications. In this review, we summarize the present knowledge about cyanobacterial terpenoid biosynthesis, both regarding the native metabolism and regarding metabolic engineering of cyanobacteria for heterologous production of non-native terpenoids.

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Section: Terpenoids Produced In Cyanobacteriamentioning

confidence: 99%

Terpenoids and Their Biosynthesis in Cyanobacteria

Pattanaik

Lindberg

2015

Life

145

103

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Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803

Lin

Saha

Zhang

et al. 2017

Sci Rep

108

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Isoprenoids are diverse natural compounds, which have various applications as pharmaceuticals, fragrances, and solvents. The low yield of isoprenoids in plants makes them difficult for cost-effective production, and chemical synthesis of complex isoprenoids is impractical. Microbial production of isoprenoids has been considered as a promising approach to increase the yield. In this study, we engineered the model cyanobacterium Synechocystis sp. PCC 6803 for sustainable production of a commercially valuable isoprenoid, limonene. Limonene synthases from the plants Mentha spicata and Citrus limon were expressed in cyanobacteria for limonene production. Production of limonene was two-fold higher with limonene synthase from M. spicata than that from C. limon. To enhance isoprenoid production, computational strain design was conducted by applying the OptForce strain design algorithm on Synechocystis 6803. Based on the metabolic interventions suggested by this algorithm, genes (ribose 5-phosphate isomerase and ribulose 5-phosphate 3-epimerase) in the pentose phosphate pathway were overexpressed, and a geranyl diphosphate synthase from the plant Abies grandis was expressed to optimize the limonene biosynthetic pathway. The optimized strain produced 6.7 mg/L of limonene, a 2.3-fold improvement in productivity. Thus, this study presents a feasible strategy to engineer cyanobacteria for photosynthetic production of isoprenoids.

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Interactions of Isoprenoid Pathway Enzymes and Indirect Stimulation of Isoprenoid Biosynthesis by Pentose Phosphate Cycle Substrates in Synechocystis PCC 6803

Cited by 2 publications

References 48 publications

Terpenoids and Their Biosynthesis in Cyanobacteria

Terpenoids and Their Biosynthesis in Cyanobacteria

Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803

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