Interactions of hyaluronan (hyaluronic acid) with phospholipids as determined by gel permeation chromatography, multi-angle laser-light-scattering photometry and 1H-NMR spectroscopy

Ghosh, Peter; Hutadilok, Nongporn; Adam, Naomi; Lentini, Aldo

doi:10.1016/0141-8130(94)90028-0

Cited by 48 publications

(39 citation statements)

References 29 publications

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“…At higher concentrations, secondary structure aggregates become entangled to form an extended three-dimensional network, the tertiary structure (33). The concentrations of HA for conversion to tertiary network structure for HA of similar molecular weights to that used in this work has been reported to be 0.1% (w/v) (34)(35).…”

Section: Nebulization Behavior Of the Suspensionsmentioning

confidence: 95%

RETRACTED ARTICLE: The Influence of Sodium Hyaluronate, l-Leucine and Sodium Taurocholate on the Nebulization of Aqueous Betamethasone-17-Valerate Suspensions

Tadros

2008

AAPS PharmSciTech

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Abstract. The purpose of this research was to evaluate the variables that are suggested to influence the adsorption of the hydrophilic hyaluronic acid (HA) onto the surface of the hydrophobic betamethasone-17-valerate (BV) particles in order to formulate a nebulizable suspension. The adsorption of HA from aqueous solutions (0.04% to 0.16%, w/v) to a fixed BV concentration (0.04%, w/v) under different experimental conditions, was investigated. The method of preparation of HA-BV suspensions involved suspending BV particles either in the hydrated HA solution (method 1) or in water followed by addition of solid HA (method 2). Other variables like the time required for the adsorption to complete and temperature at which adsorption is carried out were studied. The nebulization of the suspensions was tested via an air jet nebulizer connected to a twin stage impinger. In order to improve the nebulization behavior of the optimized suspension, L-leucine or sodium taurocholate was incorporated in increasing concentrations (0.01-0.04%, w/v). The optimized suspension, having a nebulization efficiency of 33.75%, was achieved following the adsorption of HA (0.1%, w/v) onto BV particles adopting method 2 of preparation and extending for three days at 4°C. Incorporation of either l-leucine or sodium taurocholate significantly decreased the aggregate size of the optimized suspension and consequently caused significant increases in the nebulization efficiency to reach 46.87% and 56.25%, respectively.

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Section: Nebulization Behavior Of the Suspensionsmentioning

confidence: 95%

RETRACTED ARTICLE: The Influence of Sodium Hyaluronate, l-Leucine and Sodium Taurocholate on the Nebulization of Aqueous Betamethasone-17-Valerate Suspensions

Tadros

2008

AAPS PharmSciTech

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“…More than one specific molecule contributing to the boundary lubrication of articular cartilage is not particularly surprising given the variety of interactions that can occur between the many molecules present in SF and at the articular surface (for example, see refs. 5,21,54,55).…”

Section: Discussionmentioning

confidence: 99%

Boundary lubrication of articular cartilage: Role of synovial fluid constituents

Schmidt

Gastelum

Nguyen

et al. 2007

Arthritis & Rheumatism

476

525

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Objective. To determine whether the synovial fluid (SF) constituents hyaluronan (HA), proteoglycan 4 (PRG4), and surface-active phospholipids (SAPL) contribute to boundary lubrication, either independently or additively, at an articular cartilage-cartilage interface.Methods. Cartilage boundary lubrication tests were performed with fresh bovine osteochondral samples. Tests were performed using graded concentrations of SF, HA, and PRG4 alone, a physiologic concentration of SAPL, and various combinations of HA, PRG4, and SAPL at physiologic concentrations. Static ( static, Neq ) and kinetic (< kinetic, Neq >) friction coefficients were calculated.Results. Normal SF functioned as an effective boundary lubricant both at a concentration of 100% (< kinetic, Neq > ‫؍‬ 0.025) and at a 3-fold dilution (< kinetic, Neq > ‫؍‬ 0.029). Both HA and PRG4 contributed independently to a low in a dose-dependent manner. Values of < kinetic, Neq > decreased from ϳ0.24 in phosphate buffered saline to 0.12 in 3,300 g/ml HA and 0.11 in 450 g/ml PRG4. HA and PRG4 in combination lowered further at the high concentrations, attaining a < kinetic, Neq > value of 0.066. SAPL at 200 g/ml did not significantly lower , either independently or in combination with HA and PRG4. Conclusion. The results described here indicate that SF constituents contribute, individually and in combination, both at physiologic and pathophysiologic concentrations, to the boundary lubrication of apposing articular cartilage surfaces. These results provide insight into the nature of the boundary lubrication of articular cartilage by SF and its constituents. They therefore provide insight regarding both the homeostatic maintenance of healthy joints and pathogenic processes in arthritic disease.

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“…The lubricant role of HYA in the soft tissue lining of joints is well established but its contribution on the hard weight-bearing cartilaginous surfaces is less clear. A very thin film can maintain separation of surfaces bearing a high static load [21], but reduction of friction on cartilage probably relies on a complex interplay of its surface with HYA, a particular glycoprotein of synovial fluid, and phospholipid micelles [22,23]. The functional role of anomalous elasticity is not so easily illustrated, but it seems likely to minimize cellular distortion and hasten recovery from distortion by the myriads of brief mechanical stresses to which the softer tissues of the integument are exposed.…”

Section: Functions Arising From Its Intrinsic Properties (Reviewed Inmentioning

confidence: 99%

Hyaluronan: its nature, distribution, functions and turnover

Fraser

Laurent²,

Laurent

1997

Journal of Internal Medicine

1,740

1,315

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Fraser JRE, Laurent TC, Laurent UBG (Monash University, Clayton, Victoria, Australia; and University of Uppsala, Uppsala, Sweden). Hyaluronan: its nature, distribution, functions and turnover (Minisymposium: Hyaluronan). J Intern Med 1997; 242: 27–33. Hyaluronan is a polysaccharide found in all tissues and body fluids of vertebrates as well as in some bacteria. It is a linear polymer of exceptional molecular weight, especially abundant in loose connective tissue. Hyaluronan is synthesized in the cellular plasma membrane. It exists as a pool associated with the cell surface, another bound to other matrix components, and a largely mobile pool. A number of proteins, the hyaladherins, specifically recognize the hyaluronan structure. Interactions of this kind bind hyaluronan with proteoglycans to stabilize the structure of the matrix, and with cell surfaces to modify cell behaviour. Because of the striking physicochemical properties of hyaluronan solutions, various physiological functions have been assigned to it, including lubrication, water homeostasis, filtering effects and regulation of plasma protein distribution. In animals and man, the half‐life of hyaluronan in tissues ranges from less than 1 to several days. It is catabolized by receptor‐mediated endocytosis and lysosomal degradation either locally or after transport by lymph to lymph nodes which degrade much of it. The remainder enters the general circulation and is removed from blood, with a half‐life of 2–5 min, mainly by the endothelial cells of the liver sinuoids.

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Interactions of hyaluronan (hyaluronic acid) with phospholipids as determined by gel permeation chromatography, multi-angle laser-light-scattering photometry and 1H-NMR spectroscopy

Cited by 48 publications

References 29 publications

RETRACTED ARTICLE: The Influence of Sodium Hyaluronate, l-Leucine and Sodium Taurocholate on the Nebulization of Aqueous Betamethasone-17-Valerate Suspensions

RETRACTED ARTICLE: The Influence of Sodium Hyaluronate, l-Leucine and Sodium Taurocholate on the Nebulization of Aqueous Betamethasone-17-Valerate Suspensions

Boundary lubrication of articular cartilage: Role of synovial fluid constituents

Hyaluronan: its nature, distribution, functions and turnover

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