Interactions of cytochrome P450s with their ligands

Regioselective Biocatalytic Hydroxylation of Fatty Acids by Cytochrome P450s

“…Several mutagenesis approaches showed the possibility to transform conventional P450s into peroxygenases [59,61]. Therefore, peroxygenases have high potential; however, since elevated H 2 O 2 -concentrations are harmful for both, the enzyme and its heme-cofactor [58][59][60], stable variants [59,61] and variants rescuing the heme-cofactor are highly desired. Furthermore, alternative in situ H 2 O 2 -production methods [69,133] or feeding of low amounts of H 2 O 2 may be exploited.…”

“…A significant advantage of P450 peroxygenases using H 2 O 2 is the avoidance of NAD(P)H and the possible need for additional enzymes [57]. However, P450 peroxygenases are also prone to inactivation due to the required high concentrations of hydrogen peroxide [58][59][60]. Mutagenesis approaches including directed evolution [61] and site-directed mutagenesis [59] showed successful stabilization and increased activity of peroxygenases.…”

Illuminating cytochrome P450 binding: Ru(ii)-caged inhibitors of CYP17A1

“…33–34 Ru(II)-based molecules are ideal caging groups for aromatic heterocycles because they are stable, generally non-toxic and release aromatic heterocycles with light in the visible range. 18 Due to the higher efficiency of photodissociation for 1 vs. 2 , compound 1 was chosen for a spectral shift assay under dark and light conditions to probe binding to CYP17A1.…”

Functional studies of N‐terminally modified CYP2J2 epoxygenase in model lipid bilayers

“…Hence, it is strongly believed that membrane lipid molecules may play an important role in gating the entrance of substrates and=or release of products, implying that these pathways are likely partially immersed in the membrane. 31,32 Model lipid bilayers of Nanodisc for functional interrogation of CYP2J2…”