Interactions of Butyric Acid– and Acetic Acid–Treated Salmonella with Chicken Primary Cecal Epithelial Cells In Vitro

Immerseel, Filip Van; Buck, Jeroen De; Smet, Ingrid De; Pasmans, Frank; Haesebrouck, Freddy; Ducatelle, Richard

doi:10.1637/7094

Cited by 65 publications

(47 citation statements)

References 15 publications

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“…The stationary-phase Salmonella cells exhibited reduced attachment only in the presence of SCFA at pH 6.0, while actual increases in invasion occurred for lower concentrations of acetate (342,343). Van Immerseel et al, using avian intestinal and cecal epithelial cell lines, also observed increases in invasion of S. Enteritidis exposed to acetate for 4 h and decreases with propionate and butyrate (265,344). Gantois et al reported that exposure of S. Enteritidis and S. Typhimurium to butyrate for 4 h resulted in the lowest percentage of invasion of HeLa cells (345).…”

Section: Salmonella Virulence Response and Organic Acidsmentioning

confidence: 95%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

247

218

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SUMMARY Enteric pathogens such as Salmonella enterica cause significant morbidity and mortality. S. enterica serovars are a diverse group of pathogens that have evolved to survive in a wide range of environments and across multiple hosts. S. enterica serovars such as S . Typhi, S . Dublin, and S . Gallinarum have a restricted host range, in which they are typically associated with one or a few host species, while S . Enteritidis and S . Typhimurium have broad host ranges. This review examines how S. enterica has evolved through adaptation to different host environments, especially as related to the chicken host, and continues to be an important human pathogen. Several factors impact host range, and these include the acquisition of genes via horizontal gene transfer with plasmids, transposons, and phages, which can potentially expand host range, and the loss of genes or their function, which would reduce the range of hosts that the organism can infect. S . Gallinarum, with a limited host range, has a large number of pseudogenes in its genome compared to broader-host-range serovars. S. enterica serovars such as S . Kentucky and S . Heidelberg also often have plasmids that may help them colonize poultry more efficiently. The ability to colonize different hosts also involves interactions with the host's immune system and commensal organisms that are present. Thus, the factors that impact the ability of Salmonella to colonize a particular host species, such as chickens, are complex and multifactorial, involving the host, the pathogen, and extrinsic pressures. It is the interplay of these factors which leads to the differences in host ranges that we observe today.

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Section: Salmonella Virulence Response and Organic Acidsmentioning

confidence: 95%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

247

218

View full text Add to dashboard Cite

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“…Invasion experiments were performed with the human colon carcinoma cell line T84 and with chicken primary cecal epithelial cells as previously described (43). Phagocytosis assays (29) were performed with the chicken macrophage cell line HD11 (2).…”

Section: Methodsmentioning

confidence: 99%

“…From this lysate, a 10-fold dilution series was plated on BG agar plates. Invasion and phagocytosis assays with chicken primary cells and chicken macrophage cells (HD11) were performed essentially as described above, with some modifications (29,43). All assays were performed in duplicate, with three repeats of each experiment.…”

Section: Methodsmentioning

confidence: 99%

A Live Salmonella enterica Serovar Enteritidis Vaccine Allows Serological Differentiation between Vaccinated and Infected Animals

et al. 2007

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Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic ⌬guaB mutant, a nonflagellated ⌬fliC mutant, and an auxotrophic and nonflagellated ⌬guaB ⌬fliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The ⌬fliC mutant was pathogenic in orally infected BALB/c mice, while the ⌬guaB mutant was attenuated and conferred protection against a challenge with the pathogenic parent strain. The ⌬guaB ⌬fliC double mutant was totally asymptomatic and conferred better protection than the ⌬guaB mutant. This indicates that the major flagellar protein flagellin is not required for efficient vaccination of BALB/c mice against Salmonella infection. The ⌬guaB ⌬fliC mutant was also safe for vaccination of 1-day-old chickens. After two immunizations, it induced statistically significant protection against infection of the internal organs of the birds by a virulent S. enterica serovar Enteritidis challenge strain but not against intestinal colonization. These data demonstrate that nonflagellated attenuated Salmonella mutants can be used as marker vaccines.

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“…and E.coli in the crop which is a major colonization site, but it is desirable to reach further down the intestinal tract in a sufficient concentration. van Immerseel et al (2004) tried microencapsulation and coating of propionic, formic, acetic and butyric acid in micropearls to allow the slower and selective release of the acids in the intestine of young chickens. The same authors compared the efficacy of uncoated and coated butyric acid in controlling Salmonella colonization early after oral inoculation of SpF layer chickens with Salmonella enteritidis.…”

Section: The Form Of the Organic Acidsmentioning

confidence: 99%

“…In the last decade, butyric acid was intensively studied for its role in Salmonella infections in poultry. van Immerseel et al (2004) reported the decrease of S. enteritidis invasion in caecal epithelial cells in vitro after pretreating the cells with butyric acid. On the contrary, pretreatment with acetic acid resulted in increase of invasion.…”

Section: Organic Acids Against Important Poultry Pathogensmentioning

confidence: 99%

Effects of organic acids on the gut ecosystem and on the performance of broiler chickens

Andreopoulou

Tsiouris

Georgopoulou

2017

J Hellenic Vet Med Soc

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Organic acids are studied as candidate alternatives to antibiotic growth promoters. Their action is related to the pH reduction of the intestinal digesta, affecting the gut ecosystem in numerous ways. Intestinal microbiota can be altered as a result of the remarkable antibacterial activity of organic acids and the growth enhancement of non-pathogenic beneficial microorganisms, due to exclusive competition. Antibacterial activity has been widely reported for many poultry pathogens, such as Salmonella spp., Escherichia coli, Clostridium perfringens, Campylobacter spp., both in vitro and in vivo. However, it seems to depend on many factors concerning the weak acid used and the gut ecosystem. Apart from the microbiota, diet supplementation of organic acids has trophic effects on the intestinal mucosa, modifying the morphologic characteristics of intestinal villi and crypts and maintaining epithelial integrity. Furthermore, as found recently, organic acids have anti-inflammatory and immunostimulating properties. Diet acidification increases gastric proteolysis and the utilization of proteins and amino acids, affects pancreatic secretions and mineral absorption. There are also reports for an effect on appetite and palatability of the feed. All these properties attributed to organic acids have either a direct or indirect effect on the performance and health, even though the results presented for poultry lack consistency. Nonetheless, the benefits of organic acids can have practical application in the control of clinical and subclinical conditions, but more research is needed to study these perspectives.

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Interactions of Butyric Acid– and Acetic Acid–Treated Salmonella with Chicken Primary Cecal Epithelial Cells In Vitro

Cited by 65 publications

References 15 publications

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

A Live Salmonella enterica Serovar Enteritidis Vaccine Allows Serological Differentiation between Vaccinated and Infected Animals

Effects of organic acids on the gut ecosystem and on the performance of broiler chickens

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