Interactions of biomechanics with extracellular matrix components in abdominal aortic aneurysm wall

Tanios, F.; Gee, Michael W.; Pelisek, Jaroslav; Kehl, S.; Grabher-Meier, V.; Biehler, Jonas; Wall, Wolfgang A.; Eckstein, Hans‐Henning; Reeps, Christian

doi:10.1016/j.atherosclerosis.2015.04.914

Cited by 1 publication

(6 citation statements)

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“…10 11 12 13 14 18 19 20 21 22 The current results further confirm that besides collagens PG are also significantly affected by the altered mechanical loads in AAA. 14 In particular, a significant increase of CS and DS PGs has been observed in AAA tissue samples. These ECM-associated PGs are known to have collagen-binding capacities and to modulate the formation of collagenous fibrils.…”

Section: Discussionsupporting

confidence: 83%

“…Collagenous and elastic fibers, as well as PGs, play an important role in the maintenance of mechanical stability in the aortic wall. 10 11 12 13 14 18 19 20 21 22 The current results further confirm that besides collagens PG are also significantly affected by the altered mechanical loads in AAA. 14 In particular, a significant increase of CS and DS PGs has been observed in AAA tissue samples.…”

Section: Discussionsupporting

confidence: 83%

“…Interrelated synthesis as well as binding activity could account for such correlated concentrations of endocan with Col1 and Col3 and might be an interesting cofactor in the context of a serum biomarker for AAA. 14 23…”

Section: Discussionmentioning

confidence: 99%

“…To determine the mechanical properties of the collected aortic tissue samples, tensile tests were performed as described previously. 14 16 Of all collected samples, n = 64 were suitable for these experiments. Prior to testing, specimen thickness was determined with a digital thickness gauge (Quick-Mini Series 700, Mitutoyo, Kawasaki, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies demonstrated already that altered biomechanical conditions in AAA are associated with the increased synthesis of various ECM components. 14 15…”

Section: Introductionmentioning

confidence: 99%

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Changes in Endocan and Dermatan Sulfate Are Associated with Biomechanical Properties of Abdominal Aortic Wall during Aneurysm Expansion and Rupture

et al. 2022

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Background and aims: The study aimed to assess the potential of proteoglycans (PG) and collagens as serological biomarkers in the abdominal aortic aneurysm (AAA). Furthermore, we investigated the underlying mechano-biological interactions and signaling pathways. Methods: Tissue and serum samples from patients with ruptured AAA (rAAA, n=29), elective AAA (eAAA, n=78), and healthy individuals (n=8) were evaluated by histology, immunohistochemistry and Enzyme-linked Immunosorbent Assay (ELISA), mechanical properties were assessed by tensile tests. Regulatory pathways were determined by membrane-based sandwich immunoassay. Results: In AAA samples, collagen type I and III (Col1, Col3), chondroitin sulfate (CS), and dermatan sulfate (DS) were significantly increased compared to controls (3.0-, 3.2-, 1.3-, and 53-fold; p<0.01). Col1 and endocan were also elevated in the serum of AAA patients (3.6- and 6.0-fold; p<0.01), while DS was significantly decreased (2.5-fold; p<0.01). Histological scoring showed increased total PGs and focal accumulation in rAAA compared to eAAA. Tissue β-stiffness was higher in rAAA compared to eAAA (2.0-fold, p=0.02). Serum Col1 correlated with maximum tensile force and failure tension (r=0.448 and 0.333; p<0.01 and =0.02), tissue endocan correlated with α-stiffness (r=0.340; p<0.01). Signaling pathways in AAA were associated with ECM synthesis and VSMC proliferation. In particular, Src family kinases, PDGF- and EGF-related proteins seem to be involved. Conclusions: Our findings reveal a structural association between collagen and PGs and their response to changes in mechanical loads in AAA. Particularly Col1 and endocan reflect the mechano-biological conditions of the aortic wall also in the patient’s serum and might serve for AAA risk stratification.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Our previous studies demonstrated already that altered biomechanical conditions in AAA are associated with the increased synthesis of various ECM components. 14 15…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations