Interactions of basic compounds in reversed-phase high-performance liquid chromatography influence of sorbent character, mobile phase composition, and pH on retention of basic compounds

Sýkora, David; Tesařová, Eva; Popl, M.

doi:10.1016/s0021-9673(96)00691-7

Cited by 91 publications

(48 citation statements)

References 37 publications

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“…Other approaches took advantage of the addition of silanol blockers e.g. triethylamine 21 . The optimization with mobile phase consisted of 0.1 % TFA: ACN (90:10 % v/v), flow rate 1.0 ml/min, Inertsil ODS C 18 column and isocratic technique was done.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

HPLC Method for the Determination of Ambroxol HCL in the Presence of Antimicrobial Preservatives in Oral Liquid Formulation

Sudha¹,

Manthena²,

Ravikumar³

et al. 2015

J Pharm Sci Innov

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A simple gradient reversed phase high performance liquid chromatographic method was developed for the determination of ambroxol hydrochloride in the presence of antimicrobial preservatives in oral liquid formulation. The chromatographic separation was achieved on an Inertsil C8 (250 X 4.6 mm 5 µ particle size) column using gradient elution at PDA detector. The optimized mobile phase consisted of 0.1 % Trifluoroacetic acid as a mobile phase A and a mixture of mobile phase A and acetonitrile in the ratio of (76:24 % v/v) as mobile phase B. The compounds were eluted at a flow rate of 1.0 ml/min. The method validated according to the International conference Harmonization (ICH) guidelines. The validation characteristics included accuracy, precision, linearity, specificity, limit of detection, limit of quantification and robustness. The calibration curves were linear over the (r 2 > 0.99) concentration range from 300 to 900 ppm for ambroxol hydrochloride, 10 to 30 ppm for propyl paraben and 100 to 300 ppm for methyl paraben. The percentage recoveries were found to be in the range from 99.55 to 101.1 % for ambroxol hydrochloride, 100.31 to 101.92 % for propyl paraben and 98.18 to 101.61 % for methyl paraben. Stability indicating capability was established by forced degradation experiments. No chromatographic interference from the degradation products was found. The proposed method was highly sensitive, precision and accurate and hence successfully applied for the quantification of ambroxol active pharmaceutical ingredients (API) and preservatives content in the commercially available oral liquid formulation.

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Section: Methods Development and Optimizationmentioning

confidence: 99%

HPLC Method for the Determination of Ambroxol HCL in the Presence of Antimicrobial Preservatives in Oral Liquid Formulation

Sudha¹,

Manthena²,

Ravikumar³

et al. 2015

J Pharm Sci Innov

View full text Add to dashboard Cite

show abstract

“…Other approaches took advantages of the addition of silanol blockers e.g. triethylamine (Sykora et al, 1997). The optimized chromatographic conditions were developed with Inertsil C 8 column, and mobile phase A of 0.1% TFA and mobile phase B consisted of acetonitrile and mobile phase a (76:24% v/v).…”

Section: Chromatographicmentioning

confidence: 99%

High performance liquid chromatographic method for the determination of ambroxol hydrochloride in presence of antimicrobial preservatives in oral liquid formulation

Sudha¹,

Manthena²,

Ravikumar³

et al. 2015

Bangla Pharma J

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A simple gradient reversed phase high performance liquid chromatographic method was developed for the determination of ambroxol hydrochloride in presence of antimicrobial preservatives in oral liquid formulation. The chromatographic separation was achieved by an Inertsil C 8 (250 X 4.6 mm, 5µ particle size) column using gradient technique. The eluents were detected at 245 nm with photodiode array detector. The optimized mobile phase consisted of 0.1% trifluoroacetic acid as a mobile phase A and a mixture of mobile phase A and acetonitrile in the ratio of 76:24 % v/v as mobile phase B. Ambroxol hydrochloride and microbial preservatives were eluted at a flow rate of 1.0 ml/min. The method validated according to the International Conference of Harmonization (ICH) guidelines. The calibration curves were linear over the (r 2 > 0.99) concentrations range from 300 to 900 ppm for ambroxol hydrochloride, 10 to 30 ppm for propyl paraben and 100 to 300 ppm for methyl paraben. The limit of detection was found to be 0.024 ppm for ambroxol hydrochloride, 0.018 ppm for propyl paraben and 0.009 ppm for methyl paraben. The percentage recoveries were found to be in the range from 99.55 to 101.1% for ambroxol hydrochloride, 100.31 to 101.46% for propyl paraben and 98.18 to 101.61% for methyl paraben. Stability indicating capability was established by forced degradation experiments. No chromatographic interference from the degradation products was found. The proposed method was highly sensitive, precision and accurate and hence successfully applied for the quantification of ambroxol active pharmaceutical ingredients (API) and preservatives content in the commercially available oral liquid formulation.

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“…10 The definition and determination of pH is a key question when using an aqueous solvent with organic modifier. 30 Aqueous solutions with different pH values of 2.5, 3.0, 3.5, 4.0 and 5.7, were applied in this work. The effect of pH value was investigated and showed in Fig.…”

Section: Effect Of Ph Valuementioning

confidence: 99%

Simultaneous Determination of Hesperidin, Ferulic Acid, Cinnamic Acid and Cinnamaldehyde in Chinese Tonic Wine by High Performance Liquid Chromatography

Lin¹,

Cham²,

Wu³

2010

J Chinese Chemical Soc

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This study aims to establish a HPLC method for the simultaneous determination of hesperidin (HE), ferulic acid (FA), cinnamic acid (CA) and cinnamaldehyde (CAD) in a Chinese tonic wine, Ma-Zu-WanShow-Yao-Jyo. After filtering the tonic wine, the filtrate was separated on a C18 column (250´4.6 mm, 5 mm) by gradient elution of acetonitrile-aqueous solution. The regression equations were linear (r ³ 0.9999) in the range of 1.5-150.0 mg/mL for HE, 0.3-60.0 mg/mL for FA, 0.06-6.0 mg/mL for CA, and 0.1-80.0 mg/mL for CAD. The relative standard deviation and relative error were less than 5.0% and 4.0%, respectively, for intra-and inter-day analyses. The limits of detection (LOD) were 0.6, 0.075, 0.012 and 0.02 mg/mL for HE, FA, CA and CAD (S/N = 3, 20 ml injection) respectively. This method can be used to standardize the quality control of Chinese tonic wine to ensure accuracy and efficiency in the future.

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Interactions of basic compounds in reversed-phase high-performance liquid chromatography influence of sorbent character, mobile phase composition, and pH on retention of basic compounds

Cited by 91 publications

References 37 publications

HPLC Method for the Determination of Ambroxol HCL in the Presence of Antimicrobial Preservatives in Oral Liquid Formulation

HPLC Method for the Determination of Ambroxol HCL in the Presence of Antimicrobial Preservatives in Oral Liquid Formulation

High performance liquid chromatographic method for the determination of ambroxol hydrochloride in presence of antimicrobial preservatives in oral liquid formulation

Simultaneous Determination of Hesperidin, Ferulic Acid, Cinnamic Acid and Cinnamaldehyde in Chinese Tonic Wine by High Performance Liquid Chromatography

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