Interactions of Aspergillus fumigatus with its host during invasive pulmonary infections

Latgé, Jean‐Paul; Beauvais, Anne

doi:10.1007/1-4020-5492-0_15

Cited by 2 publications

(1 citation statement)

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“…is the most useful diagnostic marker in patients with IA (17). DC-SIGN, a C-type lectin receptor present on the surface of both macrophages and dendritic cells, contains a mannan-binding domain that has been shown to bind to the galactomannan of A. fumigatus (18). This interaction reduces the T-cell stimulatory activity of dendritic cells.…”

Section: Role Of Biofilm Moleculesmentioning

confidence: 99%

Aspergillus Biofilm In Vitro and In Vivo

Beauvais

Latgé

2015

Microbiol Spectr

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In vivo, Aspergillus fumigatus grows as a typical biofilm with hyphae covered by an extracellular matrix (ECM) composed of polysaccharides, galactomannan, and galactosaminogalactan. α1,3 glucans and melanin are also constitutive of the ECM in aspergilloma but not in invasive aspergillosis. In vitro, two biofilm models were established to mimic the in vivo situation. The first model (model 1) uses submerged liquid conditions and is characterized by slow growth, while the second model (model 2) uses agar medium and aerial conditions and is characterized by rapid growth. The composition of the ECM was studied only in the second model and has been shown to be composed of galactomannan, galactosaminogalactan (GAG), and α1,3 glucans, melanin, antigens, and hydrophobins. The presence of extracellular DNA was detected in model 1 biofilm but not in model 2. Transcriptomic analysis employing both biofilm models showed upregulation of genes coding for proteins involved in the biosynthesis of secondary metabolites, adhesion, and drug resistance. However, most data on A. fumigatus biofilms have been obtained in vitro and should be confirmed using in vivo animal models. There is a need for new therapeutic antibiofilm strategies that focus on the use of combination therapy, since biofilm formation poses an important clinical problem due to their resistance to antifungal agents. Furthermore, in vivo investigations of A. fumigatus biofilms that incorporate the associated microbiota are needed. Such studies will add another layer of complexity to our understanding of the role of A. fumigatus biofilm during lung invasion.

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Section: Role Of Biofilm Moleculesmentioning

confidence: 99%