Interactions of a short hyaluronan chain with a phospholipid membrane

Bełdowski, Piotr; Yuvan, Steven; Dédinaité, Andra; Claesson, Per M.; Pöschel, Thorsten

doi:10.1016/j.colsurfb.2019.110539

Cited by 19 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 10 shows the extracted radial distribution functions around the DPPC O4 atom in the phosphate group and the important oxygen groups in HA, evaluated in sodium chloride and calcium chloride solutions. Along with the previous studies, we have chosen DPPC O4 as this group is frequently participating in H-bonds with HA [ 44 ] (for the numbering of the single oxygen species see Figure 9 ). Most of the hydrogen bonds between HA and DPPC at higher temperatures are mediated via the O10 oxygen in HA.…”

Section: Resultsmentioning

confidence: 99%

“…In the presence of calcium ions, the number of hydrogen bonds to water for HA is decreased. This can be explained by the binding of calcium ions to HA and the closer binding of HA to the DPPC bilayer in the presence of calcium ions, which reduce the possibility for water binding [ 44 ]. The distribution of distances of ions to the HA-O5 (carboxylate) and DPPC-O4 (phosphate) atoms shows more calcium ions than sodium ions in close proximity to these groups.…”

Section: Discussionmentioning

confidence: 99%

“…It was speculated that a possible mechanism is a charge reversal of the phosphate group by calcium ions resulting in a positive charge of DPPC. Indeed, theoretical calculations confirm that calcium ions promote long-lasting interactions between the carboxylate group of HA and the phosphate group of the phospholipid by creating a bridging connection [ 44 ]. Further, a reinforcement of the HA network by the adsorption of phospholipids has been observed [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC

et al. 2020

Self Cite

View full text Add to dashboard Cite

Hyaluronan is an essential physiological bio macromolecule with different functions. One prominent area is the synovial fluid which exhibits remarkable lubrication properties. However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion. Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication. We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution. We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics). The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction. Furthermore, the interaction is increased by the presence of calcium ions. Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC. The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The hydrophobic interaction strength between hydrophobic atoms (carbon based) is calculated as follows. Hydrophobic atoms are identified and assigned an atom type from the groups depicted as type 1 for carbon atoms with three or more bond hydrogen atoms (-CH3), type 2 for carbon atoms with two hydrogen atoms or one hydrogen atom plus three carbon atoms bond (-CH2-, HCC3), and type 3 for aromatic ring carbon atoms with only carbon and hydrogen atoms bond [9].…”

Section: Methodsmentioning

confidence: 99%

“…To understand the structure of albumin (and other proteins) we need to know what influence non-covalence interactions such as hydrogen bond (HB), hydrophobic contacts (HP), π-π and cation-π interactions have on the formation of the protein chain. An important aspect is also which amino acids most often interact with each other and what types of interactions they are [6][7][8][9][10]. Thus, these interactions are studied in the paper.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Protein Intramolecular and Solvent Bonding on Example of Major Synovial Fluid Component

Domino¹,

Bełdowski²,

Mazurkiewicz³

et al. 2019

Preprint

View full text Add to dashboard Cite

In this paper we review dynamics and roughness of bonds in proteins on example of albumin, that is important from the physiological point of view. We have performed computer simulations of albumin chain. Statistics were collected by performing many simulations realizations for each experimental setting. We concentrate on hydrogen bonds, cation-π and π-π interactions and NP contacts. Histograms of hydrogen bonds length are positively skewed in contrary to histograms of interactions and HP contacts that are negatively skewed. Scaling exponents of power spectra of energies of bonds / interactions /contacts are in range -0.2 to -0.5 and significantly differ between various hydrogen bonds or interactions. Varying scaling of such spectra can be used to classify between distinct bonds or contacts. Concerning particular amino-acids, largest amount of HBO H20 bonds are between Glutamate (GLU) amino-acids and water particle, while large amount of HBO bonds are formed with Lysine (LYS). For HP contacts the mayor role plays Phenylalanine (PHE) and Leucine (LEU) amino-acids. From decay curves HBO H2O bonds decays in fastest rate, while HBO bonds and HP contacts at slowest rate. We present as well decay curves of bonds formed by particular amino-acids, that gives interesting results.

show abstract

Analysis of Protein Intramolecular and Solvent Bonding on Example of Major Sonovital Fluid Component

Bełdowski

Domino

Bełdowski

et al. 2021

Biologically-Inspired Systems

View full text Add to dashboard Cite

Interactions of a short hyaluronan chain with a phospholipid membrane

Cited by 19 publications

References 31 publications

Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC

Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC

Analysis of Protein Intramolecular and Solvent Bonding on Example of Major Synovial Fluid Component

Analysis of Protein Intramolecular and Solvent Bonding on Example of Major Sonovital Fluid Component

Contact Info

Product

Resources

About