Interactions in the postprandial appearance of beta-carotene and canthaxanthin in plasma triacylglycerol-rich lipoproteins in humans

Paetau, Inke; Chen, H.; Goh, Natalie; White, Wendy S.

doi:10.1093/ajcn/66.5.1133

Cited by 62 publications

(55 citation statements)

References 35 publications

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“…These 2 peaks reflect the dynamics of the incorporation of ␤-carotene into plasma lipoproteins (23). The overall plasma appearance of ␤-carotene was lower after the sunflower-oil-rich meal than after the beeftallow-rich meal by repeated-measures ANOVA (P < 0.05).…”

Section: Postprandial Appearance Of ␤-Carotene and Retinyl Palmitatementioning

confidence: 85%

“…After the test meal, 10-mL blood samples were drawn at hourly intervals for 10 h via the intravenous catheter into a syringe and were transferred to tubes containing EDTA as an anticoagulant. The patency of the catheter was maintained by flushing with sterile physiologic saline solution, as described previously (23). During this period, water but no food was allowed with the exception of an afternoon snack that contained no vitamin A or ␤-carotene (28 g graham crackers and 122 g non-vitamin-fortified, nonfat milk).…”

Section: Study Protocolmentioning

confidence: 99%

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Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Jandacek

White

2000

The American Journal of Clinical Nutrition

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Background: Evidence indicates that different types of fat have different effects on the postprandial plasma triacylglycerol response. Therefore, the type of fat may influence the appearance of ␤-carotene in postprandial triacylglycerol-rich lipoproteins, which is used as an indicator of intestinal ␤-carotene absorption. Objective: We compared in female subjects the appearance of ␤-carotene in plasma triacylglycerol-rich lipoproteins after ␤-carotene was ingested with a meal containing sunflower oil or beef tallow. Design: Women (n = 11) each ingested 2 different vitamin A-free, fat-rich meals that were supplemented with ␤-carotene (47 mol) and contained equivalent amounts (60 g) of sunflower oil or beef tallow. Blood samples were collected hourly from 0 to 10 h; additional samples were collected at selected intervals until 528 h. In a subgroup of the women (n = 7), plasma chylomicrons and 3 subfractions of VLDLs were separated by cumulative rate ultracentrifugation. Results: The appearance of ␤-carotene in chylomicrons and in each VLDL subfraction was lower after ingestion with the meal containing sunflower oil than after ingestion with the meal containing beef tallow (P < 0.03). In chylomicrons, the area under the concentration-versus-time curve (AUC) for ␤-carotene was 38.1 ± 13.6% lower (P < 0.03); in contrast, the AUC for triacylglycerol was higher (P < 0.05) after the sunflower-oil-rich meal than after the beef-tallow-rich meal. Conclusions: Ingestion of ␤-carotene with a meal rich in sunflower oil as compared with a meal rich in beef tallow results in lower appearance of ␤-carotene and greater appearance of triacylglycerol in triacylglycerol-rich lipoproteins.

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Section: Postprandial Appearance Of ␤-Carotene and Retinyl Palmitatementioning

confidence: 85%

Section: Study Protocolmentioning

confidence: 99%

Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Jandacek

White

2000

The American Journal of Clinical Nutrition

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“…sex, smoking, disease) and there is little information on the kinetics of carotenoid depletion in humans [36], especially under pathological conditions. Serum carotenoids reflect short-term dietary intake [21] and display different depletion rates in serum depending on their chemical structure [37]. In the present study, upon a controlled very low-carotenoid intake, the drop in serum carotenoid concentrations in both type-1 diabetics and controls differed from one carotenoid to another and was related to the initial concentrations (the higher the initial concentrations, the greater the drop in serum), a finding that is consistent with previous reports involving controls [20,21,36] and individuals fed carotenoid-free enteral formulae [38] and single-dose supplementation studies [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Carotenoid Depletion in Serum of Young Type-1 Diabetics Fed Low-Carotenoid Diets

Granado

Olmedilla²,

Blanco³

2004

Ann Nutr Metab

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Background/Aims: Type-1 diabetics have been considered to be at risk for increased oxidative stress which has been implicated in the development of long-term diabetes complications. Evidence suggests that antioxidant activity may be an important mechanism by which carotenoids could confer protection in human health. Our aim was to compare the serum carotenoid depletion rate in type-1 diabetics and control subjects consuming low-carotenoid diets. Methods: Ten type-1 diabetics and 8 controls followed a very low-carotenoid diet for 21 days. Dietary intake was recorded daily and fasting blood samples collected at baseline and after 1, 2, 3, 6, 11, 15, 16, 17, and 21 days. Individual carotenoids in serum were analyzed by a validated HPLC method. Results: In both groups, carotenoid intake was less than 5% of the season-adjusted carotenoid reference intake. These diets resulted in decreased serum carotenoid levels in both groups, although depletion curves, final mean concentrations and the estimated half-life of carotenoids in serum revealed no differences between type-1 diabetics and controls. Levels of other minor serum carotenoids, cis-isomers and keto-carotenoids, also decreased whereas serum retinol and α- and γ-tocopherol did not change during the study. Conclusion: Upon a low-carotenoid intake, the depletion rate of carotenoids in serum in young type-1 diabetics does not differ from that observed in matched related controls.

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“…Possible explanations for this low response are impaired intestinal absorption of ␤-carotene, exaggerated conversion of ␤-carotene to vitamin A, inefficient incorporation of ␤-carotene into chylomicrons, or accelerated clearance of ␤-carotene due to atypical lipoprotein metabolism. Aside from the possibility that other carotenoids may interfere with the absorption and metabolism of ␤-carotene (19), the conditions that produce the low-responder trait are unknown. Variability of the conversion of ␤-carotene to vitamin A in women measured by using a double-tracer study design…”

Section: Introductionmentioning

confidence: 99%

Variability of the conversion of β-carotene to vitamin A in women measured by using a double-tracer study design

Lin

Dueker

Burri

et al. 2000

The American Journal of Clinical Nutrition

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Background: Blood ␤-carotene and vitamin A responses to oral ␤-carotene are variable in humans. Some individuals are characterized as responders and others as low-or nonresponders. A better understanding of the conditions that produce the variability is important to help design public health programs that ensure vitamin A sufficiency. Objective: Our objective was to assess variability in absorption and conversion of ␤-carotene to vitamin A in vivo in humans by using a novel double-tracer [hexadeuterated (D 6 ) ␤-carotene and D 6 retinyl acetate] approach. Design: Eleven healthy women were housed at the US Department of Agriculture Western Human Nutrition Research Center metabolic unit for 44 d, where they consumed diets adequate in vitamins and minerals except for carotenoids. After an adaptation period, the women were given 30 mol D 6 retinyl acetate orally, followed 1 wk later with 37 mol D 6 ␤-carotene (approximately equimolar doses). Time-dependent plasma concentration curves were determined for D 6 retinol, D 6 ␤-carotene, and trideuterated (D 3 ) retinol (derived from D 6 ␤-carotene). Results: Mean (± SE) absorption of D 6 ␤-carotene was 3.3 ± 1.3% for all subjects. The mean conversion ratio was 0.81 ± 0.34 mol D 3 retinol to 1 mol D 6 ␤-carotene for all subjects. However, only 6 of the 11 subjects had plasma D 6 ␤-carotene and D 3 retinol concentrations that we could measure. The mean absorption of D 6 ␤-carotene in these 6 subjects was 6.1 ± 0.02% and their conversion ratio was 1.47 ± 0.49 mol D 3 retinol to 1 mol D 6 ␤-carotene. The remaining 5 subjects were low responders with ≤ 0.01% absorption and a mean conversion ratio of 0.014 ± 0.004 mol D 3 retinol to 1 mol D 6 ␤-carotene. Conclusion: Variable absorption and conversion of ␤-carotene to vitamin A both contribute to the variable response to consumption of ␤-carotene. Our double-tracer approach is adaptable for identifying efficient converters of carotenoid to retinoid. Am J Clin Nutr 2000;71:1545-54.

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Interactions in the postprandial appearance of beta-carotene and canthaxanthin in plasma triacylglycerol-rich lipoproteins in humans

Cited by 62 publications

References 35 publications

Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Carotenoid Depletion in Serum of Young Type-1 Diabetics Fed Low-Carotenoid Diets

Variability of the conversion of β-carotene to vitamin A in women measured by using a double-tracer study design

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