Interactions between whey proteins and kaolinite surfaces

Barral, Silvia; Villa‐García, María A.; Rendueles, Manuel; Dı́az, Mario

doi:10.1016/j.actamat.2008.02.009

Cited by 44 publications

(28 citation statements)

References 21 publications

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“…However, no significant shifts were observed in digestion media powders with the zeolite treatments. Thus, result revealed that there was no hydrogen bonding and zeolite did't interact with C=O, C-N or N-H of the polypeptide chains in the digestive enzymes [34] which was also in wave number (cm -1 ) Absorbance (Arbitrary Units) Fig. 3 Infrared spectra of zeolite samples (Z control-untreated zeolite, ZG gastric treated zeolite, ZD digested zeolite) good agreement with the FTIR results of the zeolite samples.…”

Section: Characterization Resultssupporting

confidence: 60%

Investigation of structural properties of clinoptilolite rich zeolites in simulated digestion conditions and their cytotoxicity against Caco-2 cells in vitro

Kavak

Ülkü

2012

J Porous Mater

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The use of the clinoptilolite rich natural zeolites in biomedical applications such as in anticancer therapy, drug or drug support systems and as nutritive supplement is highly dependent on their behavior in digestive conditions. Aim of this study is to investigate structural stability of clinoptilolite rich natural zeolites in simulated digestion conditions and their interactions with digestive media and with Caco-2 (human colon adenocarcinoma) cells. X-ray Diffraction (XRD), Fourier Transform InfraRed (FTIR), Scanning Electron Microscopy (SEM) and Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) analyses of the clinoptilolite rich zeolite samples showed that zeolites preserved their structural stabilities during in vitro digestion. Slight interactions were detected in UV measurements of the digestive liquid media and FTIR spectra of the intestinal digested media powders. SEM results implied that zeolites might have a role in the aggregation of the digestive enzymes. Cytotoxicity test using colon cancer cells showed that clinoptilolite rich natural zeolites have cytotoxic effect against Caco-2 cells and cytotoxicity did not significantly change with respect to simulated digestion process.

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Section: Characterization Resultssupporting

confidence: 60%

Investigation of structural properties of clinoptilolite rich zeolites in simulated digestion conditions and their cytotoxicity against Caco-2 cells in vitro

Kavak

Ülkü

2012

J Porous Mater

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“…The appearance of additional carbon and nitrogen on the sepiolite surface indicate GYW protein attachment onto the sepiolite surface. Barral et al (2008) confirmed a lack of hydrogen bonding between whey proteins and the hydroxyl groups onto the surface of kaolinite using Fourier transform infrared spectroscopy. Barral et al (2008) concluded that electrostatic interactions were the primary mechanism behind protein adsorption onto kaolinite surfaces.…”

Section: Effect Of Sepiolite Additionsupporting

confidence: 67%

“…With X-ray elemental component analysis, the washed sepiolite had a similar nitrogen and carbon concentration compared to sepiolite with GYW as seen in Table 3. Efficient desorption of the proteins was not accomplished because most of the proteins remained attached to the sepiolite surface after the distilled water wash. Barral et al (2008) examined different eluents for desorbing whey proteins from a kaolinite surface: distilled water, 1 M NaCl, polyethylene glycol, ethanol, ethanol with NaCl, and ethanol with sodium dodecyl sulfate (SDS). Only ethanol with SDS was determined to be an effective eluent for protein desorption.…”

Section: Effect Of Sepiolite Additionmentioning

confidence: 99%

“…Yu et al (2013) discussed possible mechanisms for interactions between clay minerals and proteins including cation exchange, cation bridge, hydrogen bonding, hydrophobic interactions, hydrophilic interactions, van der Waals forces, and electrostatic interactions. Barral et al (2008) used a layered aluminosilicate clay mineral, kaolinite, to adsorb bovine serum albumin (BSA), beta-lactoglobulin (β-LG), and alpha- Table 3. The appearance of additional carbon and nitrogen on the sepiolite surface indicate GYW protein attachment onto the sepiolite surface.…”

Section: Effect Of Sepiolite Additionmentioning

confidence: 99%

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Value Addition of Greek Yogurt Whey Using Magnetic Fluid and Sepiolite Treatments

Kyle

Amamcharla

2015

Food Bioprocess Technol

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Magnetic fluid treatment (MFT) and the addition of a magnesium hydrosilicate clay mineral, sepiolite, were evaluated as physical cost-effective methods to add value to Greek yogurt whey (GYW), a co-product of Greek yogurt manufacturing. Three separate batches of GYW were obtained from two US Greek yogurt manufacturers. A GYW sample was collected from each batch, adjusted to a pH of 7.2, and recirculated for 5 min through a pumping system at a rate of 7.5 L/min with or without exposure to a 0.6 Tesla permanent magnetic field. The sample was split into thirds, heated to 80°C, and then sepiolite was added at one of three levels: 0, 2, or 4 g of sepiolite per 100 g GYW. After centrifugation at 1000g for 5 min, samples formed a top aqueous and a bottom sediment layer. Top aqueous layers were analyzed for total solids, ash, lactose, protein, calcium, phosphates, and sodium contents along with color. MFT did not feasibly affect the analyzed GYW components from both manufacturers. Protein within the top aqueous layers compared to GYW significantly decreased about 60 % for manufacturer 1 and 45 % for manufacturer 2 with either amount of sepiolite treatment. The color values b* and a* in the GYW from both manufacturers significantly decreased with the addition of sepiolite by about 70 and 90 %, respectively. MFT in the present study was not a practical treatment method for de-mineralizing GYW. However, sepiolite was found to have possible applications in the removal of proteins, phosphates, and natural color pigments in GYW.

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“…X-ray diffraction data for the protein-kaolinite complexes showed that protein molecules were not intercalated in the mineral structure, but immobilized at the external surfaces and the edges of the kaolinite. Protein/clay microstructure appear to be influenced by electrostatic interactions, although steric effects should be also considered [20].…”

Section: Puncture Testmentioning

confidence: 99%

Hard Biodegradable Biopolymer Obtained from Whey Protein Concentrate and Montmorillonite

Kawecka-Radomska

Tomczyńska‐Mleko

Wesołowska–Trojanowska

et al. 2015

J Polym Environ

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The aim of this study was to obtain whey protein concentrate-WPC/montmorillonite-MON biopolymers. Mixed whey protein/montmorillonite biopolymers were formed as heat-induced gels and hardened by water evaporation. Increase in protein concentration caused an increase in storage and loss moduli of the gels. Adding 5 % of MON to whey protein gel matrix caused an increase in the moduli value. Obtained biopolymers behaved as weak physical gels as loss tangent was in a range 0.25-0.45. Increase in protein concentration and addition of MON caused increase in viscosity of the biopolymers measured by dissipation of ultrasound vibrations. Addition of MON generally caused reinforcement of the structure of the mixed gels and the material was more resistant to puncture. Addition of MON caused changes in the microstructure of whey protein gel, which became more fine-stranded. It was probably caused by adsorption of the ions by MON. Drying of WPC/MON gels caused formation of very hard biopolymer, which can be applied as natural biodegradable material.

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Interactions between whey proteins and kaolinite surfaces

Cited by 44 publications

References 21 publications

Investigation of structural properties of clinoptilolite rich zeolites in simulated digestion conditions and their cytotoxicity against Caco-2 cells in vitro

Investigation of structural properties of clinoptilolite rich zeolites in simulated digestion conditions and their cytotoxicity against Caco-2 cells in vitro

Value Addition of Greek Yogurt Whey Using Magnetic Fluid and Sepiolite Treatments

Hard Biodegradable Biopolymer Obtained from Whey Protein Concentrate and Montmorillonite

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