Interactions between vascular actions of detergent and catecholamines in perfused gills of European eel, Anguilla anguilla L. and brown trout, Salmo trutta L.

Abstract

Noradrenaline induced vasodilation in isolated perfused eel or trout gills was inhibited by propranolol, a /3-adrenergic blocking agent. The noradrenaline effect was much reduced in gills of fish which had been kept in 1 mgA solutions of the detergent LAS (linear alkylate sulphonate; 12C chain length). LAS produced concentration-dependent vasodilation, which was inhibited by propranolol, in eel and trout gills. Noradrenaline produced additional vasodilation in eel and trout gills which had been dilated by 2. IO+M LAS but had no effect on trout gills perfused with 2.

Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti

“…Furthermore, they are involved in osmorregulation (Gonzales and McDonald, 1992;Flik and Verbost, 1993;Romão et al, 2001;Verbost et al, 1994), acid-base balance (Epstein et al, 1980;Evans et al, 1982;Lin and Randall, 1991;McDonald et al, 1991;Goss et al, 1992), excretion of nitrogenous compounds (Goldstein, 1982;Evans and Cameron, 1986;Sayer and Davenport, 1987), and taste (Hughes, 1982;Rios and Fanta, 1998). Organic Pesticides (Davis and Wedemeyer, 1971;Rao and Rao, 1981;Mallatt, 1985;Evans, 1987;Laurent and Perry, 1991;Nowak, 1992;Wendelaar Bonga and Lock, 1992), detergents (Schimid and Mann, 1961;Abel, 1976;Bolis and Rankin, 1980), acids (Daye and Garside, 1980;McDonald, 1983;Kawall, 1993), salts (Hossler, 1980;Luvizotto, 1994;Fanta et al, 1995), industrial waste (Mitz and Giesy, 1985;Stoker et al, 1985;Lindesjöö and Thulin, 1994), ammonia (Smart, 1976;Arillo et al, 1979;Soderberg et al, 1984) and heavy metals (Skidmore, 1970;Matthiessen and Brafield, 1973;Lock and ...…”

The fish gill: site of action and model for toxic effects of environmental pollutants.

“…with perfusate containing 0.6 to 3 ppm LAS produced a concentrationdependent vasodilation that was blocked by the 1B-adrenoceptor antagonist propranolol, and was therefore presumably via direct interaction with a 3-adrenoceptor. In a subsequent study they extended these findings to both S. trutta and A. anguilla and also found that LAS (1 ppm in acclimation medium or 2 x 10-7 M in perfusate) actually interfered with norepinephrine-induced vasodilation (102). More recently, Stagg et al (103) found that 2 x 10-8 M (6 ppb) sodium lauryl sulfate (SLS) in the perfusate reversibly (and noncompetitively) inhibited the vasodilatory action of noradrenaline on the perfused gills ofA.…”

Histological Biomarker as Diagnostic Tool for Evaluating the Environmental Quality of Guajará Bay – PA - Brazil

“…Both gill and liver are extremely important organs because they serve for respiration and osmoregulation and for regulating ion concentrations (Hinton et al, 1992), hence they play a central role in metabolism (Arellano et al, 1999). Because the gills are in direct contact with water, toxic substances can easily interfere the morphophysiology of these organs, as for instance the use of organic pesticides (Laurent & Perry, 1991), detergents (Bolis & Rankin, 1980), acids ( McDonald, 1983, salt (Fanta et al, 1995), industrial waste (Lindesjöö & Thulin, 1994), ammonia (Miron et al, 2008) and heavy metals (Oliveira Ribeiro et al, 1996 ). During the breathing process, to prevent secondary lamellae, solid agents cross the filaments during the inflow of water, however, high concentrations of irritants dissolved in water inevitably come into contact with the outer surface of the gill filaments and secondary lamellae of the current circulation, which can alter the normal gill morphology, causing cell proliferation, epithelial lifting, hypertrophy, infiltration, and aneurysm (Simonato et al, 2008).…”