Interactions between U-937 human macrophages and poly(propyleneimine) dendrimers

Kuo, Jung-hua Steven; Jan, Ming‐Shiou; Lin, Yilin

doi:10.1016/j.jconrel.2007.03.019

Cited by 50 publications

(41 citation statements)

References 30 publications

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“…A decrease in MitoTracker Orange fluorescence was also reported in HaCaT cells after PAMAM treatment [43]. It has been demonstrated that depolarization of the mitochondrial membrane occurred after cell treatment with PAMAM and this process was associated with apoptotic cell death [42,45], while PPI increased (G2) or caused fluctuations (G3) in ΔΨm [40]. Our studies showed that 24 h exposure of N2a to VPD with phosphonate surface groups led to a small depolarization of the mitochondrial membrane (Fig.…”

Section: Discussionsupporting

confidence: 77%

“…One of the cell responses to treatment with cationic unmodified dendrimers is the generation of ROS. ROS production was reported after the exposure of human macrophages to polypropyleneimine (PPI) dendrimers [40], mouse macrophages to PAMAM [41] and mouse embryonic fibroblasts to PAMAM [42]. Here, we demonstrated that the ROS level in N2a cells after VPD treatment falls below the control (Fig.…”

Section: Discussionmentioning

confidence: 53%

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Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Łaźniewska

Miłowska

Katir

et al. 2013

Cellular and Molecular Biology Letters

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Dendrimers containing viologen (derivatives of 4,4'-bipyridyl) units in their structure have been demonstrated to exhibit antiviral activity against human immunodeficiency virus (HIV-1). It has also recently been revealed that novel dendrimers with both viologen units and phosphorus groups in their structure show different antimicrobial, cytotoxic and hemotoxic properties, and have the ability to influence the activity of cholinesterases and to inhibit α-synuclein fibrillation. Since the influence of viologen-phosphorus structures on basic cellular processes had not been investigated, we examined the impact of such macromolecules on the murine neuroblastoma cell line (N2a). We selected three water-soluble viologen-phosphorus (VPD) dendrimers, which differ in their core structure, number of viologen units and number and type of surface groups, and analyzed several aspects of the cellular response. These included cell viability, generation of reactive oxygen species (ROS), alterations in mitochondrial activity, morphological modifications, and the induction of apoptosis and necrosis. The MTT assay results suggest that all of the tested dendrimers are only slightly cytotoxic. Although some changes in ROS formation and mitochondrial function were detected, the three compounds did not induce apoptosis or necrosis. In light of these results, we can assume that the tested VPD are relatively safe for mouse neuroblastoma cells. Although more research on their safety is needed, VPD seem to be promising nanoparticles for further biomedical investigation.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 53%

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Łaźniewska

Miłowska

Katir

et al. 2013

Cellular and Molecular Biology Letters

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“…Interactions between U-937 human macrophages and poly(propyleneimine) dendrimers have been recently described. In this work, these dendrimers at non-cytotoxic concentrations are shown to modify parameters such as intracellular responses-ROS content, mitochondria membrane potential, cell size and complexity, and cell cycle profiles (9).…”

Section: Introductionmentioning

confidence: 91%

Changes in Gene Expression Pattern of Human Primary Macrophages Induced by Carbosilane Dendrimer 2G-NN16

et al. 2008

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“…Recently, it has become clear that reactive oxygen species (ROS) control the cell cycle by influencing the activity of enzymes involved in cell cycle regulation [11,12]. On the other hand, it is known that some nanosystems, such as dendrimers or nanoparticles, are able to generate ROS [13,14].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Studies of Polyhedral Oligo Silsesquioxanes: Evidence for Their Low Cytotoxicity

et al. 2015

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As scientific literature considers polyhedral oligosilsesquioxanes (POSS) as potential drug delivery systems, it is necessary to check their impact on mammalian cells. Toxicity of octaammonium chloride salt of octaaminopropyl polyhedral oligomeric silsesquioxane (oap-POSS) towards two cell lines: mouse neuroblastoma (N2a) and embryonic mouse hippocampal cells (mHippoE-18) was studied. Experiments consisted of analysis of a cell cycle, cell viability, amount of apoptotic and necrotic cells, and generation of reactive oxygen species (ROS). POSS caused a shift in the cell population from the S and M/G2 phases to the G0/G1 phase. However, the changes affected less than 10% of the cell population and were not accompanied by increased cytotoxicity. POSS did not induce either apoptosis or necrosis and did not generate reactive oxygen species. A cytotoxicity profile of POSS makes it a promising starting material as drug carrier.

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Interactions between U-937 human macrophages and poly(propyleneimine) dendrimers

Cited by 50 publications

References 30 publications

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Changes in Gene Expression Pattern of Human Primary Macrophages Induced by Carbosilane Dendrimer 2G-NN16

In Vitro Studies of Polyhedral Oligo Silsesquioxanes: Evidence for Their Low Cytotoxicity

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