Interactions between trans-resveratrol and CpLIP2 lipase/acyltransferase: Evidenced by fluorescence and in silico

Nguyen, Thi-Nga; Dubreucq, Éric; Perrier, Véronique; Hung, Tran Quang; Charpentier, Claudine; Charnay, Clarence; Terki, F.; Jay‐Allemand, Christian; Bidel, Luc

doi:10.1016/j.foodchem.2020.126482

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…was still rather limited. 280,281 Strictly speaking this is a transesterification but since straightforward starting molecules as ethyl acetate of vinyl acetate are used in combination with alcohols of choice, it is viewed as ester synthesis. 7,8 In particular the acyltransferase from Mycobacterium smegmatis (MsAcT) led to a burst of new applications.…”

Section: Synthesis Of Esters and Amides In Watermentioning

confidence: 99%

Biocatalysis making waves in organic chemistry

2022

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Section: Synthesis Of Esters and Amides In Watermentioning

confidence: 99%

Biocatalysis making waves in organic chemistry

2022

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“…The fluorescence characteristics emission peak appeared at about 340 nm. With the enhancement of LMP concentration, the fluorescent intensity of lipase declined, indicating that LMP could quench the intrinsic fluorescence of lipase 31 . Study revealed that the fluorescence intensity of lipase increased with the increase of lignin concentration, which indicated that lignin could quench the intrinsic fluorescence of lipase 32 .…”

Section: Resultsmentioning

confidence: 96%

Binding mechanism of lipase with Lentinus edodes mycelia polysaccharide by multi‐spectroscopic methods

Liu

Chen

et al. 2021

J of Molecular Recognition

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It is an effective strategy to avoid obesity by inhibiting the activity of lipase. In this study, the binding mechanism of lipase and Lentinus edodes mycelia polysaccharide (LMP) were explored with multi‐spectral methods, for example, three‐dimensional (3D) fluorescence, Fourier‐transformed infrared (FT‐IR), and Raman spectra. At 290 K, the binding constant was 2.44 × 105 L/mol, there was only one binding site between LMP and lipase. Static quenching was the quenching mechanism. The major forces were hydrogen bonding and van der Waals force. The binding of LMP to lipase impacted the microenvironment around tyrosine and tryptophan residues. The polarity around these residues was decreased and hydrophobicity was enhanced. This study not only revealed the binding mechanism of LMP on lipase but also provided scientific evidence for expanding the application of LMP in functional food industries.

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“…The gene expression analysis was performed in the strain CLIB214 (CBS604), which is together with derived mutants commonly used in experimental studies (e.g. [15][16][17][18]. This strain was originally isolated from a patient with tropical diarrhea in Puerto Rico (19) and it represents the type strain of C. parapsilosis.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources

Cillingová

Tóth

Mojáková

et al. 2021

Preprint

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Many fungal species utilize hydroxyderivatives of benzene and benzoic acid as carbon sources. The yeast Candida parapsilosis metabolizes these compounds via the 3-oxoadipate and gentisate pathways, whose components are encoded by two metabolic gene clusters. In this study, we determine the chromosome level assembly of the C. parapsilosis strain CLIB214 and use it for transcriptomic and proteomic investigation of cells cultivated on hydroxyaromatic substrates. We demonstrate that the genes coding for enzymes and plasma membrane transporters involved in the 3-oxoadipate and gentisate pathways are highly upregulated and their expression is controlled in a substrate-specific manner. However, regulatory proteins involved in this process are not known. Using the knockout mutants, we show that putative transcriptional factors encoded by the genes OTF1 and GTF1 located within these gene clusters function as transcriptional activators of the 3-oxoadipate and gentisate pathway, respectively. We also show that the activation of both pathways is accompanied by upregulation of genes for the enzymes involved in β-oxidation of fatty acids, glyoxylate cycle, amino acid metabolism, and peroxisome biogenesis. Transcriptome and proteome profiles of the cells grown on 4-hydroxybenzoate and 3-hydroxybenzoate, which are metabolized via the 3-oxoadipate and gentisate pathway, respectively, reflect their different connection to central metabolism. Yet we find that the expression profiles differ also in the cells assimilating 4-hydroxybenzoate and hydroquinone, which are both metabolized in the same pathway. This finding is consistent with the phenotype of the Otf1p-lacking mutant, which exhibits impaired growth on hydroxybenzoates, but still utilizes hydroxybenzenes, thus indicating that additional, yet unidentified transcription factor could be involved in the 3-oxoadipate pathway regulation. Moreover, we propose that bicarbonate ions resulting from decarboxylation of hydroxybenzoates also contribute to differences in the cell responses to hydroxybenzoates and hydroxybenzenes. Finally, our phylogenetic analysis highlights evolutionary paths leading to metabolic adaptations of yeast cells assimilating hydroxyaromatic substrates.

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Interactions between trans-resveratrol and CpLIP2 lipase/acyltransferase: Evidenced by fluorescence and in silico

Cited by 14 publications

References 35 publications

Biocatalysis making waves in organic chemistry

Biocatalysis making waves in organic chemistry

Binding mechanism of lipase with Lentinus edodes mycelia polysaccharide by multi‐spectroscopic methods

Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources

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