Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep pyogenes. Rambouillet (n=100) and mixed breed (n=18) ewe lambs were immunized (~d42 and 22 before breeding) with PG-PS (30µgPG/kg/bw) or killed cells or were not immunized (Control, n=117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60µgPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not differ, probability of pregnancy decreased with total dose of PG-PS (p<.05). Incidence of mastitis in mixed breed ewes in the middle and at the end of lactation did not differ. In conclusion, immunization of ewe lambs with PG-PS or killed cells of Strep pyogenes did not improve pregnancy rate or incidence of mastitis after PG-PS challenge.