Interactions between the duration of stimulation and noradrenaline on cholinergic transmission in the myenteric plexus-smooth muscle preparation

Kadlec, O.; Somogyi, George T.; Šeferna, I.; Mašek, K.; Vizi, E.S.

doi:10.1016/0361-9230(86)90030-4

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…This is the opposite of the effect of high-frequency stimulation on ACh release in cardiac (Fig. 3) as well as in ileal tissues (Knoll & Vizi, 1971;Alberts & Stjiirne, 1982;Kadlec, Somogyi, Seferna, Masek & Vizi, 1986). The effect of stimulus-train duration on ACh release in the bladder was strongly influenced by the pattern of stimulation.…”

Section: Discussionmentioning

confidence: 90%

“…It is clear that MI facilitation in bladder nerves has a delayed onset, since 5 shocks delivered over a 0 5 s period did not elicit facilitation, whereas 10 shocks extending over I s produced facilitation. This response is more delayed than the presynaptic facilitation of ACh release in the guineapig ileum (Alberts & Stjiirne, 1982;Kadlec et al 1986), where the maximal facilitation occurred within I s. Furthermore, the time to reach a maximal facilitatory response in the bladder appears to be considerably longer, since the peak facilitation occurred with trains lasting 7 s (70 shocks). The delayed onset and prolonged time to reach a maximal response is consistent with the slow time course of muscarinic neuronal action (e.g.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

M1 muscarinic receptor‐mediated facilitation of acetylcholine release in the rat urinary bladder.

Somogyi

Tanowitz

Groat

1994

The Journal of Physiology

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(Somogyi & de Groat, 1992).In the present experiments we have examined ACh release in rat bladder strips with different parameters of electrical stimulation to determine whether endogenous ACh can activate MI presynaptic facilitatory receptors under more physiological conditions. We have compared ACh release in the bladder to release in the atrium, a tissue that has been studied in considerable detail (Muscholl, 1980;Bognar, Beinhauer, Kann & Fuder, 1990).A preliminary report of some of these results has appeared in an abstract (Somogyi, Tanowitz & de Groat, 1993). METHODS Preparation and incubationMale Sprague-Dawley rats (250-300 g) were killed by decapitation. The urinary bladder, and in some experiments the heart, was removed and segments weighing 10-20 mg were cut from the bladder body or from the right atrium. The tissues were placed in an incubation bath with 1 ml of Krebs solution (mM: NaCl, 113; KCl, 4-7; CaCl2, 2-5; MgSO4, 1v2; NaHCO3, 25; KH2PO4, 1P2; glucose, 11'5) containing 185 kBq ml' [3H]choline, and constantly bubbled with a mixture of 95% 02 and 5% CO2.The tissues were incubated for 30 min at 37 'C.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

M1 muscarinic receptor‐mediated facilitation of acetylcholine release in the rat urinary bladder.

Somogyi

Tanowitz

Groat

1994

The Journal of Physiology

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“…Since these responses were prevented by TTX, a blocker of axonal conduction, they should have a neuronal genesis [ 22 ]. They result from the activation of different categories of intramural neurons, leading to the local release of neurotransmitters that can have a stimulating or suppressing effect [ 23 ]. These substances interact with various cell types in the tissues, whereby each of them, in addition to direct action on SM cells, can influence processes of production and release of other neurotransmitters by other motor neurons [ 24 ], causing a complex final SM reaction.…”

Section: Discussionmentioning

confidence: 99%

Accelerated Electron Ionization-Induced Changes in the Myenteric Plexus of the Rat Stomach

Ardasheva,

Popov,

Yotov

et al. 2024

IJMS

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The influence of accelerated electrons on neuronal structures is scarcely explored compared to gamma and X-rays. This study aims to investigate the effects of accelerated electron radiation on some pivotal neurotransmitter circuits (cholinergic and serotonergic) of rats’ myenteric plexus. Male Wistar rats were irradiated with an electron beam (9 MeV, 5 Gy) generated by a multimodality linear accelerator. The contractile activity of isolated smooth muscle samples from the gastric corpus was measured. Furthermore, an electrical stimulation (200 μs, 20 Hz, 50 s, 60 V) was performed on the samples and an assessment of the cholinergic and serotonergic circuits was made. Five days after irradiation, the recorded mechanical responses were biphasic—contraction/relaxation in controls and contraction/contraction in irradiated samples. The nature of the contractile phase of control samples was cholinergic with serotonin involvement. The relaxation phase involved ACh-induced nitric oxide release from gastric neurons. There was a significant increase in serotonergic involvement during the first and second contractile phases of the irradiated samples, along with a diminished role of acetylcholine in the first phase. This study demonstrates an increased involvement of serotonergic neurotransmitter circuits in the gastric myenteric plexus caused by radiation with accelerated electrons.

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“…The effects of electrical stimulation result from the propagation of action potential, causing local release of neurotransmitters, and could comprise stimulatory and/ or inhibitory components [22,24]. Small rings of circular muscle from the rectum, with the associated enteric neurons that form functional modules [16], contracted in response to electrical stimulation.…”

Section: Discussionmentioning

confidence: 99%

Modular and functionally-different descending recto-anal motor pathways in a rat model

et al. 2011

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We evaluated the motor responses in recto-anal preparations obtained from rats, in terms of the excitation displayed by modules of nerve networks and descending distally directed pathways, when subjected to the mechanographic on-line technique, a partitioned organ bath, electrical stimulation (EFS, 0.8 ms, 5 Hz) and distension. EFS elicited modular contractions, which increased in amplitude distally, in circular muscle rings isolated from the proximal, middle or distal rectum. The modular responses of the internal anal sphincter or anal canal were relaxation or contraction, respectively. The application of EFS to the distal rectum induced a descending contractile response in the anal canal (5.24±0.34 mN), while distension by balloon evoked a descending response consisting of contraction (1.72±0.20 mN) followed by relaxation (3.42±0.24 mN). The responses were sensitive to tetrodotoxin. Atropine considerably depressed the contractions in all preparations. Whether or not atropine was present, L-NNA increased the excitatory responses, while L-arginine decreased the contractions and extended the relaxation of internal anal sphincter and anal canal. The results suggest that excitatory neurotransmission(s) expressed in the distal rectum dominate modular nerve networks. Functionally-different descending pathways are involved in the motor activity of the anal canal. Stimulatory cholinergic pathways are dependent on the electrically-induced excitation, and inhibitory nitrergic pathways are sensitive to distension of rectal wall.

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Interactions between the duration of stimulation and noradrenaline on cholinergic transmission in the myenteric plexus-smooth muscle preparation

Cited by 12 publications

References 36 publications

M1 muscarinic receptor‐mediated facilitation of acetylcholine release in the rat urinary bladder.

M1 muscarinic receptor‐mediated facilitation of acetylcholine release in the rat urinary bladder.

Accelerated Electron Ionization-Induced Changes in the Myenteric Plexus of the Rat Stomach

Modular and functionally-different descending recto-anal motor pathways in a rat model

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