Interactions between the cecal microbiota and non-alcoholic steatohepatitis using laying hens as the model

Hamid, Humera; Zhang, J Y; Li, W X; Liu, C; Li, M L; Zhao, Liang; Ji, Cheng; G, Q

doi:10.3382/ps/pey596

Cited by 48 publications

(39 citation statements)

References 62 publications

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“…The other phyla including Fusobacteria and Actinobacteria were also detected (the data was not shown), but the abundance of these bacteria was less than 1.0%. This result is in agreement with previous findings that Firmicutes, Bacteroidetes, and Proteobacteria were the 3 major bacterial phyla in the excreta sample of laying hens [12,13]. Among the major phyla, Firmicutes dominated both groups.…”

Section: Resultssupporting

confidence: 93%

“…It has been reported that a higher ratio of Bacteroidetes to Firmicutes in the GIT was related to decreasing production of short chain fatty acids (SCFA) [16]. The decreased production of SCFA in the lower part of the GIT has been reported to impair intestinal health by lowering energy supply to intestinal cells, increasing luminal pH, and decreased intestinal barrier functions [13,17].…”

Section: Resultsmentioning

confidence: 99%

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Analysis of excreta bacterial community after forced molting in aged laying hens

Han

Lee

Kang

et al. 2019

Asian-Australas J Anim Sci

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ObjectiveAs laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molting of aged hens are not clearly known. The aim of the present study was to analyze the changes in excreta bacterial communities after forced molting of aged laying hens.MethodsA total of one hundred 66-wk-old Hy-Line Brown laying hens were induced to molt by a 2-d water removal and an 11-d fasting until egg production completely ceased. The excreta samples of 16 hens with similar body weight were collected before and immediately after molting. Excreta bacterial communities were analyzed by high-throughput sequencing of bacterial 16S rRNA genes.ResultsBacteroidetes, Firmicutes, and Proteobacteria were the three major bacterial phyla in pre-molting and immediate post-molting hens, accounting for more than 98.0%. Lactobacillus genus had relatively high abundance in both group, but decreased by molting (62.3% in pre-molting and 24.9% in post-molting hens). Moreover, pathogenic bacteria such as Enterococcus cecorum and Escherichia coli were more abundant in immediate post-molting hens than in pre-molting hens. Forced molting influenced the alpha diversity, with higher Chao1 (p = 0.012), phylogenetic diversity whole tree (p = 0.014), observed operational taxonomic unit indices (p = 0.006), and Simpson indices (p<0.001), which indicated that forced molting increased excreta bacterial richness of aged laying hens.ConclusionThis study improves the current knowledge of bacterial community alterations in the excreta by forced molting in aged laying hens, which can provide increasing opportunity to develop novel dietary and management skills for improving the gastrointestinal health of aged laying hens after molting.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Analysis of excreta bacterial community after forced molting in aged laying hens

Han

Lee

Kang

et al. 2019

Asian-Australas J Anim Sci

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“…However, it is demonstrated that probiotic prevented the diminished bacterial diversity and a severe gut microbiota alteration, which is consistent with Zhou et al [37] who reported that probiotic treatment could prevent NAFLD progression via balance the gut microbiota. In the present study, the abundance of Proteobacteria was markedly enhanced by CORT exposure, and similar result has been reported by Hamid et al [33], who noted that non-alcoholic steatohepatitis (NASH) subjects exhibited an increased abundance of Proteobacteria in laying hens. A study have shown that more abundance of Proteobacteria was found in fecal of children who were high-fat, and low-ber consumers [38].…”

Section: Discussionsupporting

confidence: 92%

“…Modulation of gut microbiota with probiotics have recently been implemented in the prevention and treatment of several metabolic diseases, such as fatty liver disease [32]. Hamid et al also con rmed that general microbiota imbalance was linked with NAFLD [33]. Probiotics are considered to normalize gut microbiota and reverse microbial dysbiosis, which could potentially bene t host.…”

Section: Discussionmentioning

confidence: 99%

Probiotics Supplementation Alleviate Corticosterone-induced Fatty Liver Disease Related With the Changes of Hepatic Lipogenesis and Gut Microbiota Profile in Broiler

Mei

Feng

Yao

et al. 2020

Preprint

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Background:Emerging evidencesindicate a close relationship between gut microbiotaand fatty liver disease.It has been suggested that gut microbiota modulation with probiotics improved fatty liver disease in rodents and human, yet it still remains unclear in poultry.Results: Ninety six one-day-old green-legged chicken were divided into control group (CON) and probiotic group (PB), respectively. Probiotics were administrated throughdrinking water for two weeks from 1-day-old. At 28 d of age,16 broilers selectedfromCON or PB group randomly, and receivedvehicle(15% ethanol) or CORT(4.0 mg/kg)treatmentdaily viasubcutaneous injection for a week to induce fatty liver. At the end of the experiment, broilers from 4 groups,control group(CON), corticosterone group (CORT), probiotic group (PB), PB plusCORT group(CORT&PB),were slaughtered for sampling and analysis.The results showed that probiotics administration significantly prevented CORT-induced body weight loss(P<0.05), but did not alleviate the reduction of immune organs weight caused by CORT. Compared to CON,broilers in CORT group exhibited a significant increase of triglyceride (TG) levelin both plasma and liver(P< 0.01), as well as severe hepatocytic steatosis and hepatocellular ballooning, and accompanied with the up-regulation of hepatic lipogenesis genes expression. However, probiotics supplementation markedly decreased the intrahepatic lipid accumulation and steatosis histological score, which was associated withthedown-regulation of sterol regulatory element-binding protein-1 (SREBP1) and acetyl-CoA carboxylase (ACC) mRNA (P< 0.05)as well as it protein (P= 0.06) expression.Cecal microbiota composition was determined by 16S rRNA high-throughput sequencing. Results showed that CORT treatment induced distinct gut microbiota alterations with a decrease of microbial diversity, and anincrease of proteobacteriaabundance (P<0.05). On the contrary, probiotic supplementation increased the beta diversity and increased community richness and diversity index(P> 0.05), as well as the abundance of Intestinimonas(P<0.05).Conclusion: Our results indicate that CORT treatment induced serious fatty liver disease and altered the gut microbiota composition in broilers, however,probiotics supplementation from post-hatching had a beneficial effect on alleviatingfatty liver disease through regulating lipogenic genes expression and increasing gut microbiota diversity andbeneficial bacteria abundanceimbalance.

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“…Compared with the studies in humans and mice, studies in layers on the relationship between NAFLD and gut microbiota are rare. The findings from the limited studies in laying hens indicate that high-fat diets can induce nonalcoholic steatohepatitis and dysbiosis of gut bacteria and that the change in the abundances of Bacteroides and Lachnospiraceae genera is closely associated with the degree of liver fibrosis and the severity of nonalcoholic steatohepatitis ( Hamid et al., 2019 ). Based on these findings, we proposed a hypothesis that hepatic steatosis in laying hens was associated with the change of intestinal microbiota composition.…”

Section: Introductionmentioning

confidence: 99%

Research Note: Increase of bad bacteria and decrease of good bacteria in the gut of layers with vs. without hepatic steatosis

Yan

Liu

et al. 2020

Poultry Science

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Fatty liver hemorrhagic syndrome ( FLHS ), usually occurring in hens in the late laying period, can lower egg yield and even cause death. Similar to nonalcoholic fatty liver disease in humans, FLHS may begin with simple hepatic steatosis. It is known that gut microbiota is important to the development of nonalcoholic fatty liver disease, but the relationship between FLHS and gut bacteria remains unclear. In this study, bacteria compositions in the ileum and cecum were determined by 16S rDNA sequencing analysis in the groups of hens with vs. without hepatic steatosis (average liver fat contents were 0.34 mmol/g protein or 2.6% vs. 0.84 mmol/g protein or 6.0%). These hens were in the 2 tails of the liver fat content distribution with each tail accounting for 10% of the test population containing 90 66-wk-old healthy Rhode Island Red laying hens raised under routine feeding regimen. The results showed that liver weight but not the weights of the body, heart and abdominal fat were significantly different between the groups. Moreover, bacterial diversity was not significantly different between the groups, but bacterial diversity in the cecum was higher than that in the ileum. Proteobacteria was the most dominant phylum in the ileum, whereas Proteobacteria, Firmicutes, and Bacteroidetes were the most dominant phyla in the cecum. Some bacteria were common to the ileum and cecum, but some were unique. Furthermore, some bacteria were significantly different in abundance between the groups, that is the group without hepatic steatosis had more bacteria inhibiting host energy absorption or benefiting intestinal health, whereas the group with hepatic steatosis had more conditionally pathogenic or harmful bacteria. In conclusion, hepatic steatosis in laying hens is associated with intestinal bacterial composition (bacterial abundance) but not bacterial diversity. The identified common and unique bacteria are related to the functional characteristics of the ileum and cecum. The decrease of beneficial bacteria and the increase of harmful bacteria in the intestine of laying hens may increase FLHS incidence by promoting hepatic steatosis.

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Interactions between the cecal microbiota and non-alcoholic steatohepatitis using laying hens as the model

Cited by 48 publications

References 62 publications

Analysis of excreta bacterial community after forced molting in aged laying hens

Analysis of excreta bacterial community after forced molting in aged laying hens

Probiotics Supplementation Alleviate Corticosterone-induced Fatty Liver Disease Related With the Changes of Hepatic Lipogenesis and Gut Microbiota Profile in Broiler

Research Note: Increase of bad bacteria and decrease of good bacteria in the gut of layers with vs. without hepatic steatosis

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