Interactions Between Testicular Macrophages and Leydig Cells

HUTSON, JAMES C.

doi:10.1002/j.1939-4640.1998.tb02030.x

Cited by 28 publications

(2 citation statements)

References 51 publications

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“…Testicular macrophages are pivotal in maintaining testicular immune privilege, supporting spermatogenesis, and modulating testosterone synthesis ( 51 – 53 ). They are categorized into interstitial and peritubular macrophages based on phenotypic traits ( 54 ).…”

Section: Immuno-senescencementioning

confidence: 99%

A narrative review on inflammaging and late-onset hypogonadism

Xing,

Jin,

Jin

2024

Front. Endocrinol.

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The increasing life expectancy observed in recent years has resulted in a higher prevalence of late-onset hypogonadism (LOH) in older men. LOH is characterized by the decline in testosterone levels and can have significant impacts on physical and mental health. While the underlying causes of LOH are not fully understood, there is a growing interest in exploring the role of inflammaging in its development. Inflammaging is a concept that describes the chronic, low-grade, systemic inflammation that occurs as a result of aging. This inflammatory state has been implicated in the development of various age-related diseases. Several cellular and molecular mechanisms have been identified as contributors to inflammaging, including immune senescence, cellular senescence, autophagy defects, and mitochondrial dysfunction. Despite the extensive research on inflammaging, its relationship with LOH has not yet been thoroughly reviewed in the literature. To address this gap, we aim to review the latest findings related to inflammaging and its impact on the development of LOH. Additionally, we will explore interventions that target inflammaging as potential treatments for LOH.

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Section: Immuno-senescencementioning

confidence: 99%

A narrative review on inflammaging and late-onset hypogonadism

Xing,

Jin,

Jin

2024

Front. Endocrinol.

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“…The physical association between tMΦ and Leydig cells in the interstitium of the adult rat testis was first recognized in the late 1960s and extensively studied over the years ( Christensen, 1969 ; Hutson, 1998 ). Morphological studies revealed this direct structural interaction via morphological contact sites between the membranes of macrophages and Leydig cells, known as “digitations” ( Hutson et al, 1994 ).…”

Section: Testicular Macrophages and Their Interaction With Other Test...mentioning

confidence: 99%

Do macrophages play a role in the adverse effects of endocrine disrupting chemicals (EDCs) on testicular functions?

Cui,

Culty

2023

Front. Toxicol.

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During the past decades, several endocrine disrupting chemicals (EDCs) have been confirmed to affect male reproductive function and fertility in animal studies. EDCs are suspected to exert similar effects in humans, based on strong associations between levels of antiandrogenic EDCs in pregnant women and adverse reproductive effects in infants. Testicular macrophages (tMΦ) play a vital role in modulating immunological privilege and maintaining normal testicular homeostasis as well as fetal development. Although tMΦ were not historically studied in the context of endocrine disruption, they have emerged as potential targets to consider due to their critical role in regulating cells such as spermatogonial stem cells (SSCs) and Leydig cells. Few studies have examined the impact of EDCs on the ability of testicular cells to communicate and regulate each other’s functions. In this review, we recapitulate what is known about tMΦ functions and interactions with other cell types in the testis that support spermatogenesis and steroidogenesis. We also surveyed the literature for reports on the effects of the EDCs genistein and DEHP on tMΦ, SSCs, Sertoli and Leydig cells. Our goal is to explore the possibility that EDC disruption of tMΦ interactions with other cell types may play a role in their adverse effects on testicular developmental programming and functions. This approach will highlight gaps of knowledge, which, once resolved, should improve the risk assessment of EDC exposure and the development of safeguards to protect male reproductive functions.

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Purification of Rat Leydig Cells: Increased Yields After Unit‐Gravity Sedimentation of Collagenase‐Dispersed Interstitial Cells

SALVA,

KLINEFELTER,

HARDY

2001

Journal of Andrology

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Procedures for purification of Leydig cells have facilitated studies of their regulatory biology. A multistep procedure that includes a filtration with nylon mesh (100‐micron pore size) to separate interstitial cells from the seminiferous tubules, combining centrifugal elutriation and Percoll density gradient sedimentation, has been used to obtain a 95% enrichment of rat Leydig cells. However, the number of recovered Leydig cells by this procedure represents only a small fraction of the 25 million, on average, that exist in the adult rat testis. The objective of this study was to test whether the yield of purified Leydig cells might be enhanced by substitution of unit‐gravity sedimentation (S method) for the filter step (F method). We also asked whether a greater number of Leydig cell clusters, macrophages, or both would be recovered by this new method, and if the presence of Leydig cell clusters is associated with increased capacity for testosterone production in vitro. The number of purified Leydig cells was 1.9‐fold higher for the S method than for the F method, with no differences in purity assessed by 3β‐hydroxysteroid dehydrogenase histochemical staining. Leydig cell clusters were also found in greater numbers with the S method both after collagenase dispersion and at the end of the purification. No differences were seen in testosterone production or in the number of macrophages present in the Leydig cells that were prepared by the 2 methods. These results indicate that the new method recovers greater numbers of Leydig cells by collecting clustered Leydig cells that are systematically eliminated when a filtration step is used.

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Interactions Between Testicular Macrophages and Leydig Cells

Cited by 28 publications

References 51 publications

A narrative review on inflammaging and late-onset hypogonadism

A narrative review on inflammaging and late-onset hypogonadism

Do macrophages play a role in the adverse effects of endocrine disrupting chemicals (EDCs) on testicular functions?

Purification of Rat Leydig Cells: Increased Yields After Unit‐Gravity Sedimentation of Collagenase‐Dispersed Interstitial Cells

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