Interactions between supplemented mineral phosphorus and phytase on phytate hydrolysis and inositol phosphates in the small intestine of broilers ,

Zeller, Ellen; Schollenberger, Margit; Witzig, Maren; Shastak, Yauheni; Kühn, Imke; Hoelzle, Ludwig E.; Rodehutscord, M.

doi:10.3382/ps/pev087

Cited by 94 publications

(103 citation statements)

References 40 publications

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“…Besides determination of the enzymatic properties of a phytase, however, in vitro simulation is considered as a good complementary tool to preselect promising phytase candidates as a feed supplement. To compare the different enzyme products used in the study, the phytase activity needed to achieve a 50% reduction in IP 6 and a 50% release of the P i bound to the myo-inositol ring was estimated using nonlinear fit of a negative exponential model of the observed data (Table 3). According to the model, between 319 and 581 U phytase per kilogram of microwave-treated ground wheat was needed to achieve a 50% reduction in IP 6 , whereas between 952 and 2606 U phytase per kilogram of ground wheat was required to release 50% of the P i bound to the myo-inositol ring when the lower buffer volume was used.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract

Menezes‐Blackburn

Gabler

Greiner

2015

J. Agric. Food Chem.

110

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The aim of this study was to compare the biochemical properties of seven commercially available phytase products as well as their catalytic performance in an in vitro simulation of the digestive tract of poultry. Their enzymatic properties relevant with respect to phytate dephosphorylation in the digestive tract of birds were determined under identical assay conditions. All phytase products included in the study showed an acid pH optimum of activity and were capable of releasing the organically bound phosphate from phytate during the in vitro simulation. However, their overall biochemical properties and relative catalytic performances were remarkably different. The in vitro degradation system was considered as a simple and useful tool to evaluate the suitability of a phytase to be used as a feed supplement. Although relevant factors such as dietary P levels, intestinal phytase, and P absorption are not implemented in the system, this approach might help to reduce the number of feeding trials necessary in the search for a better suited phytase for animal feeding application.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract

Menezes‐Blackburn

Gabler

Greiner

2015

J. Agric. Food Chem.

110

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“…P is, however, a non-renewable resource that is expected to be depleted in the next 100 years (Shastak and Rodehutscord, 2013). Phytate, an organic source of P contained in plant seeds and plant-based diets for broilers, is a principal source of P for the animal, but it has the disadvantage of not being easily accessible by broilers (Witzig et al, 2015; Zeller et al, 2015). The P availability of plant-based diets can be improved by supplementing the diets with phytase, an enzyme that increases P digestibility and reduces P excretion (Witzig et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Insights into Broilers' Gut Microbiota Fed with Phosphorus, Calcium, and Phytase Supplemented Diets

et al. 2016

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Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by excretion. An enhanced P availability, and its concomitant supplementation with calcium (Ca), can affect the structure of the microbial community in the digestive tract of broiler chickens. Here, we aim to distinguish the effects of mineral P, Ca, and phytase on the composition of microbial communities present in the content and the mucosa layer of the gastrointestinal tract (GIT) of broiler chickens.Significant differences were observed between digesta and mucosa samples for the GIT sections studied (p = 0.001). The analyses of 56 individual birds showed a high microbial composition variability within the replicates of the same diet. The average similarity within replicates of digesta and mucosa samples across all diets ranged from 29 to 82% in crop, 19–49% in ileum, and 17–39% in caeca. Broilers fed with a diet only supplemented with Ca had the lowest body weight gain and feed conversion values while diets supplemented with P showed the best performance results. An effect of each diet on crop mucosa samples was observed, however, similar results were not obtained from digesta samples. Microbial communities colonizing the ileum mucosa samples were affected by P supplementation. Caeca-derived samples showed the highest microbial diversity when compared to the other GIT sections and the most prominent phylotypes were related to genus Faecalibacterium and Pseudoflavonifractor, known for their influence on gut health and as butyrate producers. Lower microbial diversity in crop digesta was linked to lower growth performance of birds fed with a diet only supplemented with Ca. Each diet affected microbial communities within individual sections, however, no diet showed a comprehensive effect across all GIT sections, which can primarily be attributed to the great variability among replicates. The substantial community differences between digesta and mucosa derived samples indicate that both habitats have to be considered when the influence of diet on the gut microbiota, broiler growth performance, and animal health is investigated.

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“…Gao et al (2013) used an even lower NPP level (1.0 g/kg, total phosphorus 3.4 g/kg) than in this study, and they also reported a higher coefficient of apparent ileal digestibility of phosphorus from this diet (0.506) compared with phosphorus digestibility from diet with total phosphorus 5.7 g/kg and 3.2 NPP g/kg (0.474). Thus, it can be inferred that the digestibility of phytate phosphorus in Zeller et al (2015) and Shastak et al (2014) who published that mineral phosphorus supplementation reduces the degradation of myoinositol phosphates. On the other hand, Hafeez et al (2016) also published very similar coefficients of apparent ileal phosphorus digestibility in layer diets (from 0.311 to 0.409).…”

Section: Discussionmentioning

confidence: 99%

Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens

Musilová¹,

Lichovnikova²,

Kupcikova³

et al. 2017

CZECH J ANIM SCI

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Musilova A., Lichovnikova M., Kupcikova L., Anderle V. (2017): Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens. Czech J. Anim. Sci., 62, 473-481.The precaecal digestibility of phosphorus and calcium in laying hens was evaluated at two dietary levels of non-phytate phosphorus (NPP; 1.58 and 2.03 g/kg) and two levels of exogenous 3-phytase (F; 0 and 300 phytase units (FTU)/kg). A total of 192 ISA Brown hens were used for the study, and were housed in enriched cages (8 per cage). At the end of the experiment, which was the peak of the egg production, the content of the posterior half of the ileum from each hen was collected. The lower level of NPP significantly (P < 0.05) improved the precaecal phosphorus digestibility coefficient. There was no significant difference in the amount of absorbed phosphorus between the lower and the higher level of NPP. The level of NPP with supplementation of exogenous phytase had a significant effect (P < 0.001) on the concentration of phosphorus in ileum, while the higher level of NPP increased phosphorus concentration in the ileum and phytase decreased phosphorus content in the ileal digesta. The phytase had no significant effect on phosphorus precaecal digestibility. There was no significant effect of the both factors, level of NPP, and exogenous phytase on the daily calcium intake, the amount of absorbed calcium, the content of calcium in ileal digesta, and on the precaecal digestibility of calcium. However a significant effect (P < 0.05) of the interaction between NPP and phytase was observed on the amount of absorbed calcium and on the precaecal digestibility coefficient. It can be concluded that the estimation of phosphorus ileal digestibility depends on the level of dietary phosphorus.

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Interactions between supplemented mineral phosphorus and phytase on phytate hydrolysis and inositol phosphates in the small intestine of broilers ,

Cited by 94 publications

References 40 publications

Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract

Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract

Insights into Broilers' Gut Microbiota Fed with Phosphorus, Calcium, and Phytase Supplemented Diets

Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens

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