Interactions between Sucrose Synthesis and CO2 Fixation IV. Temperature-dependent adjustment of the relation between sucrose synthesis and CO2 fixation

Stitt, Mark; Grosse, Heike

doi:10.1016/s0176-1617(88)80025-7

Cited by 84 publications

(100 citation statements)

References 12 publications

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“…For the mutant in low [02] the conditions were saturating for CO2 and irradiance at all temperatures and the curve indicates the temperature dependence of limitation by sucrose synthesis. The CO2 assimilation rate at 30°C was 3.5 times that at 15°C which is comparable to the factor of 2.2 reported for 02 evolution by barley at saturating CO2 and irradiance (21). (Barley is also essentially starchless [25]).…”

Section: Steady-state Photosynthesissupporting

confidence: 80%

Steady-State and Oscillating Photosynthesis by a Starchless Mutant of Nicotiana sylvestris

Hanson

1990

Plant Physiol.

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The photosynthetic characteristics of wild type Nicotiana sylvestris (Speg. et Comes) were compared with those of a 'starchless' mutant NS458 that contains a defective plastid phosphoglucomutase (EC 2.1.5.1) ( To achieve the full oscillation amplitude both irradiance and [CO2J had to exceed the minimal levels for steady-state saturation. The slowness and duration of the oscillations and the metabolic simplification introduced by deleting starch synthesis makes the mutant especially suitable for investigating the regulatory processes that generate such oscillations.longed oscillations in CO2 assimilation are readily induced for the mutant, but not for the wild type.The study of oscillations in CO2 assimilation has a long history (1O, 17, 28, 29). In 1949 Van der Veen (23,24) showed that oscillations could be generated by exposing leaves to brief periods of darkness and studied the influence of

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Section: Steady-state Photosynthesissupporting

confidence: 80%

Steady-State and Oscillating Photosynthesis by a Starchless Mutant of Nicotiana sylvestris

Hanson

1990

Plant Physiol.

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“…There was, therefore, no obvious correlation between the respiratory rate and the availability of (Fig. 4) (11,21) used to offset the limitation by phosphate at low temperatures (9). The accumulation phates which has been observed during ing in potato tubers (15) could also be a metabolically related phenomenon.…”

Section: Metabolite Measurementsmentioning

confidence: 99%

“…A number of temperature-dependent metabolic factors may act to bring about this imbalance between the capacity to fix CO2 and to synthesize sucrose. First, low temperatures raise thresholds for the metabolism of triose-P to sucrose because of large decreases in the affinity of the cytosolic Fru 1,6-P2ase3 for its substrate as the temperature is decreased (21). There is also the possibility that inactivation of the cytosolic Fru 1,6-P2ase occurs in vivo at low temperatures (23).…”

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confidence: 99%

Influence of Low Temperature on Respiration and Contents of Phosphorylated Intermediates in Darkened Barley Leaves

Labate¹,

Leegood²

1989

Plant Physiol.

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The aim of this work was to examine the effect of exposure of leaves to low temperatures (5°C) upon the contents of phosphorylated intermediates and respiration in darkened barley (Hordeum vulgare L.) plants which differed in their carbohydrate status. In leaves that had previously been illuminated for 24 hours, there was a large increase in amounts of phosphorylated metabolites at 50C during the first 3 hours of darkness, compared with control plants kept at 30°C. Hexose phosphates accounted for about two-thirds of this increase, which reached a peak after about 3 hours. At higher temperatures, there was a peak in the amount of fructose 2,6-bisphosphate and the rate of respiration which accompanied the transient increase in phosphorylated intermediates. At 50C the increase in phosphorylated intermediates was not accompanied by appreciable changes in fructose 2,6-bisphosphate, and there was a rapid decline in the rate of respiration. Leaves that had previously been darkened for 24 hours and that were low in carbohydrate failed to accumulate phosphorylated intermediates when exposed to low temperatures. The results are discussed with respect to the acclimation of carbohydrate metabolism to low temperatures. The results suggest that respiratory carbohydrate metabolism is strictly controlled even when the carbohydrate supply and glycolytic intermediates are abundant. The possibility that accumulation of hexose phosphates may be involved in acclimation of metabolism to low temperature is discussed.Low temperatures will influence carbohydrate metabolism in leaves in a number of different ways. In the whole plant, low temperatures will both diminish sink activity and export of carbohydrate from leaves, disrupting the balance between supply of, and demand for, assimilated carbon. Such temperature-dependent decreases in the utilization of photosynthate have been shown to lead to end-product inhibition of photosynthesis (1, 2, 4). (14). Exposure to low temperatures will also have consequences which occur purely at the metabolic level. For example, during photosynthesis the balance between sucrose synthesis and carbon fixation is perturbed when leaves are suddenly transferred to low temperatures because sucrose synthesis runs too slowly at lower temperatures (9,12). A number of temperature-dependent metabolic factors may act to bring about this imbalance between the capacity to fix CO2 and to synthesize sucrose. First, low temperatures raise thresholds for the metabolism of triose-P to sucrose because of large decreases in the affinity of the cytosolic Fru 1,6-P2ase3 for its substrate as the temperature is decreased (21). There is also the possibility that inactivation of the cytosolic Fru 1,6-P2ase occurs in vivo at low temperatures (23). The consequence of either change would be that more phosphorylated intermediates would be required in the cytosol in order to drive sucrose synthesis. Second, it is likely that more orthophosphate is required in the cytosol at low temperatures (1 1, 12, 21) to allow export of t...

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“…Photosynthesis is a complex metabolic network. Limitations appear in subprocesses with a particularly large Q 10 like Suc synthesis (Stitt and Grosse, 1988;Sharkey et al, 1995), while restrictions in the use of light energy lead to photoinhibition and oxidative stress (Niyogi, 1999;Nishiyama et al, 2006;Lawlor and Tezara, 2009). These direct negative effects are modified by acclimation.…”

Section: Introductionmentioning

confidence: 99%

Metabolism and Growth in Arabidopsis Depend on the Daytime Temperature but Are Temperature-Compensated against Cool Nights

et al. 2012

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Diurnal cycles provide a tractable system to study the response of metabolism and growth to fluctuating temperatures. We reasoned that the response to daytime and night temperature may vary; while daytime temperature affects photosynthesis, night temperature affects use of carbon that was accumulated in the light. Three Arabidopsis thaliana accessions were grown in thermocycles under carbon-limiting conditions with different daytime or night temperatures (12 to 24°C) and analyzed for biomass, photosynthesis, respiration, enzyme activities, protein levels, and metabolite levels. The data were used to model carbon allocation and growth rates in the light and dark. Low daytime temperature led to an inhibition of photosynthesis and an even larger inhibition of growth. The inhibition of photosynthesis was partly ameliorated by a general increase in protein content. Low night temperature had no effect on protein content, starch turnover, or growth. In a warm night, there is excess capacity for carbon use. We propose that use of this capacity is restricted by feedback inhibition, which is relaxed at lower night temperature, thus buffering growth against fluctuations in night temperature. As examples, the rate of starch degradation is completely temperature compensated against even sudden changes in temperature, and polysome loading increases when the night temperature is decreased.

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Interactions between Sucrose Synthesis and CO2 Fixation IV. Temperature-dependent adjustment of the relation between sucrose synthesis and CO2 fixation

Cited by 84 publications

References 12 publications

Steady-State and Oscillating Photosynthesis by a Starchless Mutant of Nicotiana sylvestris

Steady-State and Oscillating Photosynthesis by a Starchless Mutant of Nicotiana sylvestris

Influence of Low Temperature on Respiration and Contents of Phosphorylated Intermediates in Darkened Barley Leaves

Metabolism and Growth in Arabidopsis Depend on the Daytime Temperature but Are Temperature-Compensated against Cool Nights

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