Interactions between rGO/TNT nanocomposites and cells: Regulation of cell morphology, uptake, cytotoxicity, adhesion and migration

Su, Xiaopeng; Yang, Lifeng; Huang, Cailian; Hu, Qinli; Shan, Xinyi; Wan, Junmin; Hu, Zhiwen; Wang, Bing

doi:10.1016/j.jmbbm.2017.10.014

Cited by 11 publications

(8 citation statements)

References 47 publications

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“…As has been previously described, overactivation of fibroblasts may promote scar formation, thereby increasing fibrosis and collagen metabolism disorders 59, 60. However, Christy et al 53 and Bing et al 50 reported that 1% of GO may inhibit the migration and adhesion of fibroblasts by increasing ROS levels. In this study, the N-Col-GO membrane enhanced the sustained release of NAC as an antioxidant to reduce GO cytotoxicity, which improved the migration and adherence of fibroblasts in the early phase due to reduced ROS levels.…”

Section: Discussionmentioning

confidence: 89%

“…However, the biotoxicity of GO is controversial based on previous studies. For instance, Bing and coworkers reported that GO had low toxicity on cell cytoskeletal organization in Raw264.7 mouse monocyte-macrophage cells 50. Wierzbicki et al also found that GO did not exert strong toxicity, although it effectively deregulated cell migration and adhesion 51.…”

Section: Discussionmentioning

confidence: 99%

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N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing

Li¹,

Zhou²,

Luo³

et al. 2019

Theranostics

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Wound dressings composed of natural polymers, such as type I collagen, possess good biocompatibility, water holding capacity, air permeability, and degradability, and can be used in wound repair. However, due to the persistent oxidative stress in the wound area, the migration and proliferation of fibroblasts might be suppressed, leading to poor healing. Thus, collagen-containing scaffolds are not suitable for accelerated wound healing. Antioxidant N-acetyl cysteine (NAC) is known to reduce the reactive oxygen species (ROS) and has been widely used in the clinic. Theoretically, the carboxyl group of NAC allows loading of graphene oxide (GO) for sustained release and may also enhance the mechanical properties of the collagen scaffold, making it a better wound-dressing material. Herein, we demonstrated an innovative approach for a potential skin-regenerating hybrid membrane using GO incorporated with collagen I and NAC (N-Col-GO) capable of continuously releasing antioxidant NAC.Methods: The mechanical stability, water holding capacity, and biocompatibility of the N-Col-GO hybrid membrane were measured in vitro. A 20 mm rat full-skin defect model was created to evaluate the repair efficiency of the N-Col-GO hybrid membrane. The vascularization and scar-related genes in the wound area were also examined.Results: Compared to the Col only scaffold, N-Col-GO hybrid membrane exhibited a better mechanical property, stronger water retention capacity, and slower NAC release ability, which likely promote fibroblast migration and proliferation. Treatment with the N-Col-GO hybrid membrane in the rat wound model showed complete healing 14 days after application which was 22% faster than the control group. HE and Masson staining confirmed faster collagen deposition and better epithelization, while CD31 staining revealed a noticeable increase of vascularization. Furthermore, Rt-PCR demonstrated decreased mRNA expression of profibrotic and overexpression of anti-fibrotic factors indicative of the anti-scar effect.Conclusion: These findings suggest that N-Col-GO drug release hybrid membrane serves as a better platform for scarless skin regeneration.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing

Li¹,

Zhou²,

Luo³

et al. 2019

Theranostics

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“…Graphene is a two-dimensional (2D) nanomaterial composed of sp 2 -hybridized carbon atoms arranged in a hexagonal honeycomb shape, and it has a large specific surface area, good flexibility, high thermal conductivity, high carrier mobility, and good biocompatibility. Therefore, it can be employed as a multifunctional material for many biomedical applications , including biosensing, tissue scaffold engineering, antimicrobial therapies, bioimaging, and PTT .…”

Section: Recently Developed Nanomaterials For Pttmentioning

confidence: 99%

Review of Functionalized Nanomaterials for Photothermal Therapy of Cancers

Bian

Wang

Pan

et al. 2021

ACS Appl. Nano Mater.

107

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Photothermal therapy (PTT) is recognized as a promising approach for cancer theranostics via the nonradiative conversion of light into heat energy. PTT treatment is able to reduce the adverse side effects of traditional chemotherapy. Some nanomaterials functionalized with unique physical and chemical properties have been integrated with multiple imaging modalities and a therapeutic function for applications. In the past decade, various nanomaterials for PTT applications have been reviewed, but a comprehensive survey of all classes of photothermal nanomaterials developed in recent years has not been done. A comprehensive discussion of PTT mechanisms using different nanomaterials and their application in combination therapy is useful for providing insights for PTT material development for disease treatment in the future. In this review, the recent advancement of functionalized nanomaterials for PTT and the excellence of PTT combined therapies in the field of anticancer are discussed. The momentous property of nanomaterials tailored for advancing the noninvasive therapeutic approach of PTT is also highlighted. Because a great deal of PTT nanomaterials have been developed in the past decades and reviewed in recent years, in this review, we only include the latest results reported in the past 5 years for discussion and comparison.

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“…Nano-drug delivery systems have opened up new avenues for cancer treatment and diagnosis. 6 Nanocarriers can increase drug enrichment in tumours by enhancing permeability and retention (EPR) effects to improve specific selectivity. [7][8][9] In particular, stimulus-response drug delivery systems can control the timing and quantitative release of drugs to tumour tissues by identifying endogenous or exogenous stimulation signals.…”

Section: Introductionmentioning

confidence: 99%

ICG/5-Fu coencapsulated temperature stimulus response nanogel drug delivery platform for chemo-photothermal/photodynamic synergetic therapy

et al. 2021

Self Cite

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The multiple diagnosis and treatment mechanisms of chemotherapy combined with photothermal/photodynamic therapy have very large application prospects in the field of cancer treatment. Therefore, in order to achieve effective and safe antitumour treatment, it is necessary to design an intelligent responsive polymer nanoplatform as a drug delivery system. Herein, the thermosensitive poly-N-isopropylacrylamide (PNIPAM) nanogel particles were prepared by soap-free emulsion polymerization and loaded with a large amount of photosensitizer indocyanine green (ICG) and anticarcinogen 5-fluorouracil (5-Fu), which effectively to realize the cooperative chemotherapy and photothermal/photodynamic therapy for tumours. The 5-Fu@ICG-PNIPAM nanogels significantly improved the bioavailability of the drug and achieved controlled release. In addition, under near-infrared laser (NIR) irradiation at 808 nm, 5-Fu@ICG-PNIPAM nanogels generated lots of heat and reactive oxygen, which significantly enhanced cellular uptake and in vitro antitumour treatment effects. The results showed that 5-Fu@ICG-PNIPAM nanogels were effectively endocytosed by HeLa cells, which also enhanced the drug’s entrance into the nucleus. Moreover, compared with alone chemotherapy or photothermal/photodynamic therapy, 5-Fu@ICG-PNIPAM nanogels significantly increased cytotoxicity under NIR irradiation, suggesting that chemotherapy and photothermal/photodynamic synergistic therapy had excellent antitumour properties. Therefore, this temperature-responsive nanogel platform probably has great application prospects in clinical antitumour treatment.

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Interactions between rGO/TNT nanocomposites and cells: Regulation of cell morphology, uptake, cytotoxicity, adhesion and migration

Cited by 11 publications

References 47 publications

N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing

N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing

Review of Functionalized Nanomaterials for Photothermal Therapy of Cancers

ICG/5-Fu coencapsulated temperature stimulus response nanogel drug delivery platform for chemo-photothermal/photodynamic synergetic therapy

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