Interactions between puerarin/daidzein and micellar casein

Abstract

Puerarin (PUE) and daidzein (DAI) are polyphenols with extensive biological activities. In the present study, the interactions between PUE/DAI and micellar casein (MC) were investigated, and the physicochemical properties of their complexes were analyzed. The results of fluorescence spectrum analysis and molecular docking revealed that the main interactions between DAI and MC were hydrophobic forces, while that between PUE and MC was hydrogen bonding. The FTIR and XRD analyses confirmed the formation of complexes between MC and PUE/DAI. After binding to PUE/DAI, the size of MC increased. The weight loss rate of MC decreased after complexing with PUE/DAI, but its morphology was not extensively modified. The DPPH radical scavenging capacities of PUE-MC and DAI-MC complexes were higher than those of free PUE/DAI in both water and ethanol. In vitro release experiments showed that the release rate of PUE/DAI was inhibited by MC under simulated intestinal conditions. Practical applicationsThe low water solubility and poor bioavailability of PUE and DAI limit their application. Micellar casein has high affinity for PUE and DAI. After encapsulated by micellar casein, the release rates of PUE and DAI were prolonged during simulated intestinal digestion. The results would provide useful information for improving the solubility and bioavailability of PUE and DAI, and broadening the use of them in the food and pharmaceutical industry.

Functional pH-Sensitive Film Containing Purple Sweet Potato Anthocyanins for Pork Freshness Monitoring and Cherry Preservation

“…The spectrum of SA/CMC-Na film had a diffraction peak at 20.96°, compared with 20.34° in pure CMC-Na and 19.84° in pure SA, indicating the formation of intermolecular interactions between SA and CMC-Na [ 20 ]. The diffraction peak intensities of films were slightly higher than those of the individual components, suggesting the formation of intermolecular hydrogen bonds in the films [ 22 ]. After addition to a film, the PPE diffraction peak disappeared, indicating that PPE was well dispersed in the film matrix.…”

“…XRD spectra of films were carried out with an XD3 X-ray polycrystal diffractometer (Beijing Purkinje General Instrument Co., Ltd., Beijing, China) under Cu Kα radiation in a continuous mode. Specific parameters were a scanning range from 10 • to 80 • (2θ), voltage of 40 kV, current of 30 mA, step size of 0.02 • , and scanning speed of 5 • /min [22].…”

Mucoadhesive and thermosensitive Bletilla striata polysaccharides/chitosan hydrogel loaded nanoparticles for rectal drug delivery in ulcerative colitis

“…According to the FT-IR spectra of the two NPs (Fig. 2C and F), Pur had an aromatic conjugated carbonyl absorption band at 1633 cm − 1 , and an aromatic nucleus absorption band at 1515 cm − 1 and 1448 cm − 1 [32]. In addition, there was a peak of 891 cm − 1 in the ngerprint of Pur, which belonged to the substitution region of benzene ring [33].…”

Anti-Inflammatory Effects and Immunomodulatory Efficacy of Unitein (Fermented Glycine max, Panax ginseng, and Chenpi Mixture) in RAW 264.7 Macrophages and Mice Splenocytes along with Component Analysis

“…Rubiadin demonstrates antioxidant and hepatoprotective efects [80]. Daidzein, after soybean fermentation, converts to its nonglycoside form (daidzin), which can alleviate menopausal symptoms and lower blood cholesterol [81]. Crocin 3 possesses antioxidant and neuroprotective properties [82].…”