Interactions between parasites and their hosts: Metabolic aspects

Mettrick, D. F.

doi:10.1016/0020-7519(87)90032-4

Cited by 8 publications

(5 citation statements)

References 58 publications

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“…Similar wide differences in K m values of Mg-ATP and F-6-P were also observed with the enzyme isolated from other parasites such as M. expansa [32], A. suum [15] T. brucei [21], and T. cruzi [20] whereas the enzyme from vertebrate tissues exhibited very less difference between the K m values of Mg-ATP and F-6-P [33]. The binding of the substrate Mg-ATP to PFKs from different origins shows a hyperbolic dependence with half saturation values in the range of 10–100 μ M in most of the cases.…”

Section: Discussionsupporting

confidence: 75%

Kinetic Characterisation of Phosphofructokinase Purified fromSetaria cervi: A Bovine Filarial Parasite

Sharma

2011

Enzyme Research

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Phosphofructokinase (PFK), a regulatory enzyme in glycolytic pathway, has been purified to electrophoretic homogeneity from adult female Setaria cervi and partially characterized. For this enzyme, the Lineweaver-Burk's double reciprocal plots of initial rates and D-fructose-6-phosphate (F-6-P) or Mg-ATP concentrations for varying values of cosubstrate concentration gave intersecting lines indicating that K m values for F-6-P (1.05 mM) and ATP (3 μM) were independent of each other. S. cervi PFK, when assayed at inhibitory concentration of ATP (>0.1 mM), exhibited sigmoidal behavior towards binding with F-6-P with a Hill coefficient (n) value equal to 1.8 and 1.7 at 1.0 and 0.33 mM ATP, respectively. D-fructose-1,6-diphosphate (FDP) competitively inhibited the filarial enzyme: K i and Hill coefficient values being 0.18 μM and 2.0, respectively. Phosphoenolpyruvate (PEP) also inhibited the enzyme competitively with the K i value equal to 0.8 mM. The Hill coefficient values (>1.5) for F-6-P (at inhibitory concentration of ATP) and FDP suggested its positive cooperative kinetics towards F-6-P and FDP, showing presence of more than one binding sites for these molecules in enzyme protein and allosteric nature of the filarial enzyme. The product inhibition studies gave us the only compatible mechanism of random addition process with a probable orientation of substrates and products on the enzyme surface.

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Section: Discussionsupporting

confidence: 75%

Kinetic Characterisation of Phosphofructokinase Purified fromSetaria cervi: A Bovine Filarial Parasite

Sharma

2011

Enzyme Research

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“…The helminths in the hindgut of equids are attached to the intestinal mucosa and may suck blood (14,15). The adult stages of parasitic helminths in the definitive host all have a predominantly anaerobic energy metabolism with an array of excretory products such as lactate, ethanol, succinate, and volatile fatty acids (acetate and propionate) (18,27). Most of the nitrogen excreted is in the form of ammonia (50 to 60%).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the microbial community colonizing the anal and vulvar pores of helminths from the hindgut of zebras

Mackie

Krecek

et al. 1989

Appl Environ Microbiol

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Scanning and transmission electron microscopy were used to examine the adherence and in situ morphology of the microbial community colonizing the anal and vulvar pores of the subfamily Cyathostominae (Nematoda: Strongylidae) from the colon of Burchell's zebra (Equus burchelli antiquorum). Two different morphological types of asporogenous rod were prominent in the microbial community. One was a thin, septate, filamentous organism (0.4 to 0.5 ,um by 2 to 3 ,um) with blunt ends, which was more prominent at the site of attachment. The other was a larger (1.8 to 2.4 ,um by 5 to 10 ,im) multicellular rod with round ends in the form of a trichome. Spiraland vibrio-shaped bacteria were also present in the thin sections. The septate filaments were shown to contain a cell spacer similar to those described in Methanospirillum hungatei. Attachment to the cuticle was by means of an amorphous electron-dense material with fibrillar appearance and not by specialized holdfast segments. Ten isolates were obtained from a habitat-simulating medium on which a homogenate from the posterior region was plated. Antibodies were raised to whole cells of five rod-shaped isolates in rabbits and fluorescein isothiocyanate labeled. Positive bright-yellow fluorescence was obtained with one of the clostridial isolates. The results are discussed with reference to other bacteria with similar morphology, the nature of this unique interrelationship between the microbial community and its parasitic host inside the equine hindgut, and the possibility of biological control of parasitic helminths.

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“…Multicellular parasites, such as gastrointestinal nematodes, have been shown to divert nutrients and modify the host's appetite in order to promote their development and growth (127). Heligmosomoides polygyrus, for instance, reduces glucose transport across the intestinal mucosa, ensuring the monosaccharide's availability in the intestinal lumen (128).…”

Section: The Plasticity Of Immune Cells Is Altered In Helminthic Infe...mentioning

confidence: 99%

Similarities and divergences in the metabolism of immune cells in cancer and helminthic infections

Esperante,

Gutiérrez,

Issa

et al. 2023

Front. Oncol.

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Energetic and nutritional requirements play a crucial role in shaping the immune cells that infiltrate tumor and parasite infection sites. The dynamic interaction between immune cells and the microenvironment, whether in the context of tumor or helminth infection, is essential for understanding the mechanisms of immunological polarization and developing strategies to manipulate them in order to promote a functional and efficient immune response that could aid in the treatment of these conditions. In this review, we present an overview of the immune response triggered during tumorigenesis and establishment of helminth infections, highlighting the transition to chronicity in both cases. We discuss the energetic demands of immune cells under normal conditions and in the presence of tumors and helminths. Additionally, we compare the metabolic changes that occur in the tumor microenvironment and the infection site, emphasizing the alterations that are induced to redirect the immune response, thereby promoting the survival of cancer cells or helminths. This emerging discipline provides valuable insights into disease pathogenesis. We also provide examples of novel strategies to enhance immune activity by targeting metabolic pathways that shape immune phenotypes, with the aim of achieving positive outcomes in cancer and helminth infections.

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Interactions between parasites and their hosts: Metabolic aspects

Cited by 8 publications

References 58 publications

Kinetic Characterisation of Phosphofructokinase Purified fromSetaria cervi: A Bovine Filarial Parasite

Kinetic Characterisation of Phosphofructokinase Purified fromSetaria cervi: A Bovine Filarial Parasite

Characterization of the microbial community colonizing the anal and vulvar pores of helminths from the hindgut of zebras

Similarities and divergences in the metabolism of immune cells in cancer and helminthic infections

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