Interactions between liposomes and hydroxypropylmethylcellulose

Rubalcava, Concepción Gutiérrez de; Rodriguez, J. L.; Duro, R.J.; Álvarez-Lorenzo, Carmen; Concheiro, Ángel; Seijo, Begoña

doi:10.1016/s0378-5173(00)00433-6

Cited by 13 publications

(11 citation statements)

References 36 publications

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“…Furthermore, cholesterol could enhance the hydrophobicity of liposomal surface and therefore the hydrophobic interaction between the polymer and the liposome would mainly take place at the membrane surface instead of translocation through the membrane. 27 This hypothesis was validated by the DLS results in Table S1, showing that after the surface coating with PP75 the hydrodynamic diameter of the liposome containing 40%…”

Section: Effect Of Cholesterolmentioning

confidence: 59%

“…In the absence of cholesterol, the hydrophobic segments of the polymer chain are not stable in the aqueous solution and tend to be embedded into the apolar region of the lipid membrane. 26 However, the addition of cholesterol could condense the lipid packing 27 , change the membrane fluidity to make it more rigid 25 , and reduce the permeability of liposomes 28,29 . As a result, cholesterol could hamper the insertion of the hydrophobic parts of the polymer into the liposomal membrane.…”

Section: Effect Of Cholesterolmentioning

confidence: 99%

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A Virus-Mimicking, Endosomolytic Liposomal System for Efficient, pH-Triggered Intracellular Drug Delivery

Chen

2016

ACS Appl. Mater. Interfaces

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A novel multifunctional liposomal delivery platform has been developed to resemble the structural and functional traits of an influenza virus. Novel pseudo-peptides were prepared to mimic the pHresponsive endosomolytic behavior of influenza viral peptides through grafting a hydrophobic amino acid, L-phenylalanine, onto the backbone of a polyamide, poly(L-lysine isophthalamide), at various degrees of substitution. These pseudo-peptidic polymers were employed to functionalize the surface of cholesterol-containing liposomes that mimic the viral envelope. By controlling the cholesterol proportion, as well as the concentration and amphiphilicity of the pseudo-peptides, the entire payload was rapidly released at endosomal pHs whilst there was no release at pH 7.4. A pH-triggered, reversible change in liposomal size was observed and the release mechanism was elucidated. In addition, the virus-mimicking nanostructures efficiently disrupted the erythrocyte membrane at pH 6.5 characteristic of early endosomes, whilst showed negligible cytotoxic effects at physiological pH. The efficient intracellular delivery of the widely used anticancer drug doxorubicin (DOX) by the multifunctional liposomes was demonstrated, leading to significantly increased potency against HeLa cancer cells over the DOX-loaded bare liposomes. This novel virus-mimicking liposomal system, with the incorporated synergy of efficient liposomal drug release and efficient endosomal escape, is favorable for efficient intracellular drug delivery.3

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Section: Effect Of Cholesterolmentioning

confidence: 59%

Section: Effect Of Cholesterolmentioning

confidence: 99%

A Virus-Mimicking, Endosomolytic Liposomal System for Efficient, pH-Triggered Intracellular Drug Delivery

Chen

2016

ACS Appl. Mater. Interfaces

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“…In another approach differential scanning calorimetry was used to examine the polymer-liposome interactions (18). It is also very noteworthy the work of de Rubalcava et al (19) who made a detailed examination on the interactions between liposomes and hydoxypropylmethylcellulose.…”

Section: Introductionmentioning

confidence: 97%

Interaction Between Liposomes and Neutral Polymers: Effect of Adsorption on Drug Release

Dékány

Csóka

Erös

2001

Journal of Dispersion Science and Technology

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The processes of adsorption of two neutral polymers (poly(vinyl pyrrolidone), PVP and poly(vinyl alcohol), PVA) were investigated on liposomes composed of soy lecithin=dicetyl phosphate=cholesterol ¼ 25:2:3 (molar ratio). The liposomes were prepared in buffered solution at pH ¼ 7.4 and mixed with the solution of the measured polymers in the desired polymer=lipid (w=w) ratios. Adsorption was measured by determination of the equilibrium bulk concentration of the polymer. In the case of PVA quantitative adsorption measurements with a specific reagent were possible. Adsorption isotherms were recorded at 25 AE 1 C. It was concluded that adsorbed and unadsorbed PVA molecules are in equilibrium even at low polymer= lipid ratios. The results were confirmed by dynamic laser light scattering (DLS), and thermal activity monitoring (TAM) experiments. Another group of the liposomes was prepared in 60 mM ammonium sulphate (pH ¼ 5.0) and we filled the vesicles with a test dye, acridine orange (AO) using the pH-gradient (remote loading) method. The AO release property of liposomes was tested with a special vertical diffusion cell after we had made PVA adsorb on their surface in different PVA=lipid (w=w) ratios.

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“…To overcome this discrepancy, Takeuchi and coworkers have tried to develop more effective liposomal formulations, including polymer-coated liposomes, as drug delivery carriers (Takeuchi et al 1996(Takeuchi et al , 2001(Takeuchi et al , 2003(Takeuchi et al , 2005a. There have also been several reports concerning modified liposomal formulations (Gutierrez de Rubalcava et al 2000;Katayama et al 2003;Kim et al 1999;Li et al 2003). Charged polymers can form complexes with oppositely charged liposomes to produce so-called polymer-liposome complexes.…”

Section: Introductionmentioning

confidence: 99%