Interactions between imazethapyr and bovine serum albumin: Spectrofluorimetric study

Synthesis and structure of new dicopper(II) complexes bridged by N-(2-hydroxy-5-methylphenyl)-N′-[3-(dimethylamino)propyl]oxamide with in vitro anticancer activity: A comparative study of reactivities towards DNA/protein by molecular docking and experimental assays

“…Before measurements, the mixture was shaken up and incubated at room temperature for 30 min. Fluorescence spectra of EB bound to HS-DNA were obtained at an emission wavelength of 584 nm in the fluorometer, and the inner-filter effect (IFE) was corrected according to the literature method [23].…”

“…Thus, EB can be used to probe the interaction of metal complexes with DNA, which will provide indirect evidence for an intercalative mode as well as the binding strength [48]. It should be pointed out that inner-filter effect (IFE), which refers to the absorbance of light at the excitation or emission wavelength by other compounds present in the solution, may also lead to the decrease in fluorescence intensity, causing errors in data interpretation [23]. Thereby, it is necessary to consider the IFE and eliminate its interference.…”

Synthesis and structure of a new ternary monocopper(II) complex containing mixed ligands of 2,2′‐diamino‐4,4′‐bithiazole and picrate:in vitroanticancer activity, molecular docking and reactivity towards DNA

“…The fluorescence spectra of EB bound to HS-DNA were obtained at an emission wavelength of 584 nm using a spectrofluorimeter, keeping the concentration of HS-DNA constant while varying the concentration of the copper(II) complex, and the inner-filter effect (IFE) was corrected according to a literature method. [28] The electrochemical titration experiment was also performed by keeping the concentration of the complex constant while varying the HS-DNA concentration, using a solvent of 50 mM NaCl/Tris-HCl buffer solution at pH = 7.16. [29] All voltammetric experiments were performed in a singlecompartment cell.…”

“…If the complex can intercalate into DNA, it will lead to a decrease in the binding sites of DNA available for EB, and hence to a quenching of fluorescence intensity of the EB-DNA system. It should be pointed out that the IFE, [28] which refers to the absorption of light at the excitation or emission wavelength by other compounds present in the solution, may also lead to a decrease in fluorescence intensity. The decrease in the fluorescence intensity of a solution induced by the occurrence of the IFE could cause a nonlinear relationship between the observed fluorescence intensity and the concentration of the fluorophore, as well as result in the introduction of large errors in the data interpretation.…”

Study of the Binding of Thiazolyazoresorcinol and Thiazolyazocresol Dyes with BSA and Adenine by Spectral, Electrochemical and Molecular Docking Methods

“…For a static quenching interaction, the relationship between the fluorescence intensity and the concentration of quencher can be usually described by deriving to obtain the binding constant (Ka) and the number of binging sites (n) can be gotten from the following double logarithm regression curve [22,23]:…”