Interactions between endothelial cells and a poly(carbonate-silsesquioxane-bridge-urea)urethane

Punshon, Geoffrey; Vara, Dina S.; Sales, Kevin M.; Kidane, Asmeret G.; Salacinski, Henryk J.; Seifalian, Alexander M.

doi:10.1016/j.biomaterials.2005.03.034

Cited by 93 publications

(72 citation statements)

References 30 publications

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“…[ 3 H] thymidine incorporation is a method used for assessment of cellular proliferation, but this method is avoided due to toxicity and relatively high cost [32]. Alamar Blue is used to measure the cellular redox potential and advantageous as compared to MTT assay due to simpler sample preparation [33]. But the success of the Alamar Blue is hindered due to unavailability of the biochemical mechanisms of the assay and reaction of non-porous silicon with Alamar Blue in the absence of the cells [34].…”

Section: Proliferation Assaysmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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Section: Proliferation Assaysmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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“…Many previous studies have proven that POSS-PCU is an anti-thrombogenic [42], biocompatible [43] and non-toxic [44,45] biomaterial. It does not cause inflammatory reactions in the surrounding host tissue [46][47][48][49]. Experiments in cardiac implants also prove that POSS-PCU has a fine quality of calcification resistance (Alobaid et al, 2006;Motwani et al, 2011).…”

Section: Polyhedral Oligomeric Silsesquioxane Polycaprolactone-urea Umentioning

confidence: 97%

Intracranial Stents Past, Present and the Future Trend: Stents Made with Nano-particle or Nanocomposite Biomaterials

Zhao¹,

Kalaskar²,

Farhatnia³

et al. 2014

CMC

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“…Little information exists on vascular cell interactions on Bionate 1 (PCU) and Elasthane TM (PEU). 6,7,21,22 The effects of coating Pellethane TM , a different type of poly(ether urethane) and Bionate 1 (PCU) with fibronectin and plasma proteins, on the adhesion of macrophages has revealed that these attachment factors were essential to mediate adhesion during a 3 h time period. 22 In contrast, our work showed that endothelial cells are also able to attach and spread on uncoated PCU and PEU surfaces when cultured for at least 20 h. These differences in cellular adhesion on uncoated polyurethanes may be related to the different cell types or culture times examined.…”

Section: Human Coronary Artery Endothelial Cellsmentioning

confidence: 99%

“…5 Previous studies on medical-grade polyurethanes mainly focused on the adhesion of inflammatory cells that are known to participate in polyurethane biodegradation 6 and endothelial cell attachment and retention. 7 In spite of the critical role…”

Section: Introductionmentioning

confidence: 97%

Polyurethane biomaterials for fabricating 3D porous scaffolds and supporting vascular cells

Grenier

Sandig

Mequanint

2007

J Biomedical Materials Res

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Successful tissue engineering of vascular grafts largely depends on synthetic scaffolds that support the survival, proliferation, and differentiation of seeded cells. To investigate the utility of polyurethanes for vascular tissue engineering, three-dimensional porous polyurethane scaffolds with highly interconnected pore structures were fabricated by a pressure differential/particulate leaching technique. Ammonium chloride and paraffin porogens were prepared to fabricate the scaffolds. Grinding of ammonium chloride resulted in particulates with uniform particle sizes but irregular shapes. Paraffin particulates made by a dispersion method, on the other hand, had spherical shapes and uniform particle sizes. Polyurethane scaffolds fabricated from these particulates had open faced, highly interconnected channels that could allow cellular infiltration and nutrient delivery. Human coronary artery smooth muscle and endothelial cell interactions with polyurethane surfaces revealed these biomaterials to maintain the contractile phenotype of human coronary artery smooth muscle cells and the formation of endothelial monolayers. During longer culture times, surface modification with cell adhesive extracellular matrix (ECM) protein promoted vascular cell proliferation, maintenance of the differentiated phenotype and endothelial monolayer integrity. Our results suggest that these polyurethanes, in conjunction with cell adhesive ECM proteins, could also support vascular cells in three-dimensional bioreactor-based culture conditions.

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Interactions between endothelial cells and a poly(carbonate-silsesquioxane-bridge-urea)urethane

Cited by 93 publications

References 30 publications

In vitro and in vivo toxicity assessment of nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

Intracranial Stents Past, Present and the Future Trend: Stents Made with Nano-particle or Nanocomposite Biomaterials

Polyurethane biomaterials for fabricating 3D porous scaffolds and supporting vascular cells

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