Interactions Between Dna and Psoralenamines Studied With Dichroism Techniques

Nordén, Bengt; Wirth, Michael; Ygge, Birgitta; Buchardt, Ole; Nielsen, Peter E.

doi:10.1111/j.1751-1097.1986.tb04713.x

Cited by 7 publications

(1 citation statement)

References 23 publications

(11 reference statements)

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“…Since the product of the last two factors in equation 74is equal to pP Y -/t 2 x it corresponds to a ' local linear dichroism' of the intercalator in the base plane of DNA. Equation 74has been used to support conclusions about the orientation of intercalators within the intercalation pocket Palumbo et al 1984;Norden et al 1986c). Kubista et al (1987Kubista et al ( , 1988 applied the non-degenerate coupled oscillator expression to pair-wise combination of the transition moment of a groove-bound chromophore with the transitions of varying number of DNA bases; a strong dependence on the displacement from the DNA helix was noticed and generally a larger number of bases had to be included for convergence than with an intercalator.…”

Section: Covalent Adductsmentioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

359

376

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This review will consider solution studies of structure and interactions of DNA and DNA complexes using linear dichroism spectroscopy, with emphasis on the technique of orientation by flow. The theoretical and experimental background to be given may serve, in addition, as a general introduction into the state of the art of linear dichroism spectroscopy, particularly as it is applied to biophysical problems.

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Section: Covalent Adductsmentioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

359

376

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Induced CD of DNA intercalators: Electric dipole allowed transitions

1987

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SynopsisThe induced CD of an electric dipole allowed transition of a DNA intercalator has been calculated using the "matrix method" and a set of DNA na* transitions recently adopted for calculating the CD of DNA by Rizzo and Schellman [(1984) BwpoZymers 23, 435-4701. The induced CD is strongly dependent on the angular orientation of the intercalator and only moderately dependent on its location within the intercalation pocket. The dependence of the CD on the orientation and location of the intercalator was studied for some representative conformations of di-and tetranucleotide duplexes of (dGdC) and (dAdT). The effect of alternative DNA transition moment directions was also tested. The orientation dependence compares well with the previously predicted 1-2cos2 y dependence [B. Nordkn and F. memeld (1982) Siopolymrs 21, 1713-17341. Measured induced CD spectra of methylene blue (MB) intercalated in double-stranded poly(dAdT), poly(dGdC), and calf-thymus DNA are discussed on the basis of the results of the calculations. Major differences between the induced CD spectra are likely to reflect different modes of intercalation for the different nucleotide sequences. In particular, the concluded geometry in solution for MB intercalated in poly(dAdT) differs significantly from the corresponding geometry found in dinucleotide-intercalator crystals.

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DNA binding of a spermine derivative: Spectroscopic study of anthracene‐9‐carbonyl‐n¹‐spermine with poly[d(G‐C)·(d(G‐C))] and poly[d(A‐T) · d(A‐T)]

Rodger¹,

Blagbrough

Adlam

et al. 1994

Biopolymers

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The binding of polyamines, including spermidine (1) and spermine (2), to poly[d(G-C).d(G-C)] was probed using spectroscopic studies of anthracene-9-carbonyl-N1-spermine (3); data from normal absorption, linear dichroism (LD), and circular dichroism (CD) are reported. Ligand LD and CD for transitions located in the DNA region of the spectrum were used. The data show that 3 binds to DNA in a manner characteristic of both its amine and polycyclic aromatic parts. With poly[(dG-dC).(dG-dC)], binding modes are occupied sequentially and different modes correspond to different structural perturbations of the DNA. The most stable binding mode for 3 with poly[d(G-C).d(G-C)] has a site size of 6 +/- 1 bases, and an equilibrium binding constant of (2.2 +/- 1.1) x 10(7) M-1 with the anthracene moiety intercalated. It dominates the spectra from mixing ratios of approximately 133:1 until 6:1 DNA phosphate: 3 is reached. The analogous data for poly[d(A-T).d(A-T)] between mixing ratios 36:1 and 7:1 indicates a site size of 8.3 +/- 1.1 bases and an equilibrium binding constant of (6.6 +/- 3.3) x 10(5) M-1. Thus, 3 binds preferentially to poly[d(G-C).d(G-C)] at these concentrations.

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Interactions Between Dna and Psoralenamines Studied With Dichroism Techniques

Cited by 7 publications

References 23 publications

Linear dichroism spectroscopy of nucleic acids

Linear dichroism spectroscopy of nucleic acids

Induced CD of DNA intercalators: Electric dipole allowed transitions

DNA binding of a spermine derivative: Spectroscopic study of anthracene‐9‐carbonyl‐n¹‐spermine with poly[d(G‐C)·(d(G‐C))] and poly[d(A‐T) · d(A‐T)]

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Interactions Between Dna and Psoralenamines Studied With Dichroism Techniques

Cited by 7 publications

References 23 publications

Linear dichroism spectroscopy of nucleic acids

Linear dichroism spectroscopy of nucleic acids

Induced CD of DNA intercalators: Electric dipole allowed transitions

DNA binding of a spermine derivative: Spectroscopic study of anthracene‐9‐carbonyl‐n1‐spermine with poly[d(G‐C)·(d(G‐C))] and poly[d(A‐T) · d(A‐T)]

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DNA binding of a spermine derivative: Spectroscopic study of anthracene‐9‐carbonyl‐n¹‐spermine with poly[d(G‐C)·(d(G‐C))] and poly[d(A‐T) · d(A‐T)]