Interactions between denture lining material, protein pellicles and Candida albicans

Nikawa, Hiroki; Hayashi, Saihou; Nikawa, Y.; Hamada, Takashi; Samaranayake, LP

doi:10.1016/0003-9969(93)90132-6

Cited by 79 publications

(105 citation statements)

References 19 publications

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“…They noted that C. albicans accounted for only a small fraction of the cells of the predominately bacterial biofilm. Like the report of Ahearn and coworkers (171), there were several additional reports in the early 1990s of C. albicans in mixed biofilms forming on a variety of materials, including silicone rubber prostheses (172), impression materials (173), catheters (174), and dentures (175)(176)(177). However, it was Hawser and Douglas (168) who developed the first in vitro model of C. albicans biofilm formation, in which the biofilm was formed by and composed solely of that species.…”

Section: The Beginnings Of Candida Albicans Biofilm Researchmentioning

confidence: 87%

Plasticity of Candida albicans Biofilms

Soll

Daniels

2016

Microbiol Mol Biol Rev

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SUMMARYCandida albicans, the most pervasive fungal pathogen that colonizes humans, forms biofilms that are architecturally complex. They consist of a basal yeast cell polylayer and an upper region of hyphae encapsulated in extracellular matrix. However, biofilms formedin vitrovary as a result of the different conditions employed in models, the methods used to assess biofilm formation, strain differences, and, in a most dramatic fashion, the configuration of the mating type locus (MTL). Therefore, integrating data from different studies can lead to problems of interpretation if such variability is not taken into account. Here we review the conditions and factors that cause biofilm variation, with the goal of engendering awareness that more attention must be paid to the strains employed, the methods used to assess biofilm development, every aspect of the model employed, and the configuration of theMTLlocus. We end by posing a set of questions that may be asked in comparing the results of different studies and developing protocols for new ones. This review should engender the notion that not all biofilms are created equal.

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Section: The Beginnings Of Candida Albicans Biofilm Researchmentioning

confidence: 87%

Plasticity of Candida albicans Biofilms

Soll

Daniels

2016

Microbiol Mol Biol Rev

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“…The presence of serum or salivary pellicles, which are normally found in the oral environment and may provide binding sites for Candida (8,10), increased the initial adherence of C. dubliniensis cells to biomaterials and subsequent biofilm formation. Other investigators have previously shown that the presence of serum and salivary pellicles can potentiate C. albicans colonization of acrylic strips and denture lining materials (41)(42)(43)(44)(45)(46). SEM techniques revealed that similar to C. albicans biofilms (5, 23), mature C. dubliniensis biofilms consist of a mixture of yeast and filamentous forms embedded within exopolymeric material.…”

Section: Discussionmentioning

confidence: 99%

“…Other forms of mucosal candidiasis are frequently encountered in other patient groups, such as denture wearers, cancer patients, infants, and the elderly (11). In order to colonize and infect the oral environment, yeast cells must first adhere to host cells and tissues or prosthetic materials within the oral cavity or must coaggregate with the oral microbiota (7,8,10,20,27,28,31,42,53,57). In the case of C. dubliniensis these adhesive properties may be facilitated by the intrinsic cell surface hydrophobicity displayed by this organism (26).…”

mentioning

confidence: 99%

Biofilm Formation by Candida dubliniensis

Ramage¹,

Walle

Wickes³

et al. 2001

J Clin Microbiol

167

156

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Candida dubliniensis is an opportunistic yeast closely related to Candida albicans that has been recently implicated in oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. Here, we report on the development of in vitro models of C. dubliniensis biofilms on the surfaces of biomaterials (polystyrene and acrylic) and on the characteristics associated with biofilm formation by this newly described species. Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. Serum and saliva preconditioning films enhanced the initial attachment of C. dubliniensis and subsequent biofilm formation. Scanning electron microscopy and confocal scanning laser microscopy were used to further characterize C. dubliniensis biofilms. Mature C. dubliniensis biofilms consisted of a dense network of yeasts cells and hyphal elements embedded within exopolymeric material. C. dubliniensis biofilms displayed spatial heterogeneity and an architecture showing microcolonies with ramifying water channels. Antifungal susceptibility testing demonstrated the increased resistance of sessile C. dubliniensis cells, including the type strain and eight different clinical isolates, against fluconazole and amphotericin B compared to their planktonic counterparts. C. dubliniensis biofilm formation may allow this species to maintain its ecological niche as a commensal and during infection with important clinical repercussions.

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“…Salivary proteins and glycoproteins can act as receptors for binding of C. albicans cells to enamel surfaces (Cannon et al, 1995b) and to denture acrylic surfaces (Vasilas et al, 1992 ;Edgerton et al, 1993 ;Nikawa et al, 1993) and can modulate the binding of yeast cells to buccal epithelial cells (Kimura & Pearsall, 1978 ;Samaranayake & MacFarlane, 1982). In addition, C. albicans binding to oral viridans streptococci (Jenkinson et al, 1990 ;Holmes et al, 1995aHolmes et al, , b, 1996 and to Actinomyces (Grimaudo et al, 1996) may be enhanced by salivary components Grimaudo et al, 1996).…”

Section: Discussionmentioning

confidence: 99%