Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum

Meene, Allison van de; McAloney, Lauren; Wilson, Sarah M.; Zhou, Ji; Zeng, Wei; McMillan, Paul J.; Bacic, Antony; Doblin, Monika S.

doi:10.3390/cells10010127

Cited by 8 publications

(4 citation statements)

References 83 publications

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“…The seedlings were then transferred to mock (MS) or 500 µM ATP-containing plates for 3 days. After the treatment, the seedlings were incubated in an aqueous solution of 1% calcofluor white (CFW) for 5 minutes, 25 , 26 washed thrice with PBS, and imaged with a Leica DMI6000 microscope (Leica, Wetzlar, Germany).…”

Section: Methodsmentioning

confidence: 99%

FERONIA orchestrates P2K1-driven purinergic signaling in plant roots

Sowders,

Jewell,

Tanaka

2024

Plant Signaling & Behavior

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Section: Methodsmentioning

confidence: 99%

FERONIA orchestrates P2K1-driven purinergic signaling in plant roots

Sowders,

Jewell,

Tanaka

2024

Plant Signaling & Behavior

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“…The staining intensity was measured with imagej . For calcofluor white (CFW) staining, seedlings were incubated in an aqueous solution of 1% CFW for 5 min [32,33], washed thrice with PBS, and imaged with the Leica DMI600.…”

Section: Methodsmentioning

confidence: 99%

The intrinsically disordered C‐terminus of purinoceptor P2K1 fine‐tunes plant responses to extracellular ATP

et al. 2023

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P2K1 is a plant‐specific purinoceptor that perceives extracellular ATP (eATP), a signaling molecular implicated in various physiological processes. Interestingly, P2K1 harbors a C‐terminal intrinsically disordered region (IDR). When we overexpressed a truncated P2K1 (P2K1t) lacking the IDR, primary root growth completely ceased in response to eATP. We investigated the functional roles of the IDR in P2K1 using a combination of molecular genetics, calcium imaging, gene expression analysis, and histochemical approaches. We found that the P2K1t variant gave rise to an amplified response to eATP, through accumulation of superoxide, altered cell wall integrity, and ultimate cell death in the primary root tip. Together, these observations underscore the significant involvement of the C‐terminal tail of P2K1 in root growth regulation.

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“…In situ methods will be essential in future investigations of these variables. Emerging confocal, super-high resolution, and atomic force microscopy technologies ( van de Meene et al 2021 ) will undoubtedly allow these questions to be addressed.…”

Section: Subcellular Location Of (13;14)-β-glucan Synthesismentioning

confidence: 99%

Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans

Hrmova,

Zimmer,

Bulone

et al. 2023

Plant Physiology

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Recent breakthroughs in structural biology have provided valuable new insights into enzymes involved in plant cell wall metabolism. More specifically, the molecular mechanism of synthesis of (1,3;1,4)-β-glucans, which are widespread in cell walls of commercially important cereals and grasses, has been the topic of debate and intense research activity for decades. However, an inability to purify these integral membrane enzymes or apply transgenic approaches without interpretative problems associated with pleiotropic effects has presented barriers to attempts to define their synthetic mechanisms. Following the demonstration that some members of the CslF sub-family of GT2 family enzymes mediate (1,3;1,4)-β-glucan synthesis, the expression of the corresponding genes in a heterologous system that is free of background complications has now been achieved. Biochemical analyses of the (1,3;1,4)-β-glucan synthesized in vitro, combined with 3-dimensional (3D) cryogenic-electron microscopy and AlphaFold protein structure predictions, have demonstrated how a single CslF6 enzyme, without exogenous primers, can incorporate both (1,3)- and (1,4)-β-linkages into the nascent polysaccharide chain. Similarly, 3D structures of xyloglucan endo-transglycosylases and (1,3;1,4)-β-glucan endo- and exohydrolases have allowed the mechanisms of (1,3;1,4)-β-glucan modification and degradation to be defined. X-ray crystallography and multi-scale modeling of a broad specificity GH3 β-glucan exohydrolase recently revealed a previously unknown and remarkable molecular mechanism with reactant trajectories through which a polysaccharide exohydrolase can act with a processive action pattern. The availability of high-quality protein 3D structural predictions should prove invaluable for defining structures, dynamics, and functions of other enzymes involved in plant cell wall metabolism in the immediate future.

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Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum

Cited by 8 publications

References 83 publications

FERONIA orchestrates P2K1-driven purinergic signaling in plant roots

FERONIA orchestrates P2K1-driven purinergic signaling in plant roots

The intrinsically disordered C‐terminus of purinoceptor P2K1 fine‐tunes plant responses to extracellular ATP

Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans

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