Interactions between bovine endothelial cells and Pasteurella multocida: association and invasion

Galdiero, Massimiliano; Martino, Luisa De; Pagnini, Ugo; Pisciotta, Maria-Grazia; Galdiero, Emilia

doi:10.1016/s0923-2508(00)01168-2

Cited by 22 publications

(20 citation statements)

References 26 publications

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“…In agreement with our findings, the invasion of Chang epithelial cells by H. influenzae (St Geme & Falkow, 1990) and of bovine aortic endothelial cells by Pasteurella multocida (Galdiero et al, 2001) was found to be dependent on microfilament and microtubule formation. Thus, internalization of other members of the family Pasteurellaceae may involve similar microfilament and microtubule reorganizations.…”

Section: Discussionsupporting

confidence: 91%

“…This is likely to be the case for H. parasuis since the intracellular bacteria survive inside PBMEC but their number decreases gradually with time. It can be hypothesized that H. parasuis undergoes exocytosis after invasion as described for other pathogens, such as P. multocida with bovine aortic cells (Galdiero et al, 2001). However, degraded H. parasuis bacteria were exceptionally observed inside PBMEC by TEM (data not shown), indicating that the decreasing number of live intracellular bacteria could also be due in part to bacterial death.…”

Section: Discussionmentioning

confidence: 81%

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Haemophilus parasuis invades porcine brain microvascular endothelial cells

et al. 2006

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Haemophilus parasuis, an important swine pathogen, is the aetiological agent of Glässer's disease. It is responsible for cases of polyserositis, meningitis and pneumonia in young pigs. To date, 15 serotypes have been described, although several non-typable isolates are frequently recovered from diseased animals. The pathogenesis of H. parasuis infection is poorly understood. To cause meningitis, H. parasuis would have to cross the blood–brain barrier (BBB), composed of brain microvascular endothelial cells (BMEC). The objective of this study was to investigate the ability of H. parasuis to interact with porcine brain microvascular endothelial cells (PBMEC). It was demonstrated that the serotype 5 reference strain of H. parasuis, Nagasaki (originally recovered from a case of meningitis), was able to adhere at very high levels to and, most importantly, invade PBMEC. These capacities were confirmed by electron microscopy. Actinobacillus pleuropnemoniae serotype 7 (strain WF 83), used as negative control, was not able to adhere to or invade PBMEC. Comparisons of the levels of adhesion and invasion by several H. parasuis field strains from different serotypes isolated from cases of either meningitis or pneumonia showed that isolates of serotypes 4 and 5 had a higher invasion capacity than isolates belonging to other serotypes. Inhibition studies demonstrated that PBMEC invasion by H. parasuis required rearrangement of actin microfilaments and microtubular cytoskeletal elements but not active bacterial DNA, RNA or protein synthesis. Characterization studies demonstrated that proteinaceous invasin(s) does not seem to play a major role in entry of H. parasuis into PBMEC. Intracellular viable H. parasuis were found in PBMEC up to 6 h after antibiotic treatment. Even at high bacterial doses, H. parasuis was not toxic to PBMEC. In swine, the invasion of endothelial cells of the BBB may play an important role in the pathogenesis of meningitis caused by H. parasuis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 81%

Haemophilus parasuis invades porcine brain microvascular endothelial cells

et al. 2006

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“…In addition, taking together adherence and invasion studies, results approxi-258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 mately two to three-fold greater than those obtained by Bouchet et al (2009) were showed by us. Vanier et al (2006) showed that the number of internalized organisms decreased gradually in a time-dependent manner, and suggested an exocytosis mechanism for explaining this reduction, as already reported for Pasteurella multocida (Galdiero et al, 2001). The attachment and invasion abilities exhibited in this study by the very virulent Nagasaki strain and the absence of both capacities revealed by the nonvirulent SW114 strain are in quite agreement with those previously reported by Aragó n et al (2010) who, using PBMEC/C1-2 cells, showed that H. parasuis virulent strains derived from systemic lesions were more invasive than nonvirulent strains isolated from the nasal cavities of piglets free of Glä sser's disease.…”

Section: Discussionsupporting

confidence: 74%

Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

Frandoloso

Martínez-Martínez

Gutiérrez-Martín

et al. 2012

Veterinary Microbiology

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“…In contrast, two reports demonstrated that the invasion of P. multocida into bovine endothelial cells and MDCK cells could be reduced up to 60% by pretreatment with cytochalasin D (20,38). Interestingly, these conflicting results were also observed with L. pneumophila, in that cytochalasin D , and 24 h postinfection.…”

Section: Discussionmentioning

confidence: 93%

“…The invasive ability of P. multocida has long been debated, and only a few studies have investigated the intracellular potential of this pathogen. Virulent atrophic rhinitis and fowl-choleracausing strains were reported to invade and survive within various host cell lines such as turkey kidney epithelial cells and avian mononuclear phagocytic cells (20,31,38,49). P. multocida rabbit strains were shown to enter nasal epithelial and endothelial cells and reside within membrane-bound vacuoles in the cytoplasms of these cells (3).…”

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confidence: 99%

Interaction of Pasteurella multocida with Free-Living Amoebae

Hundt

Ruffolo

2005

Appl Environ Microbiol

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Pasteurella multocida is a highly infectious, facultative intracellular bacterium which causes fowl cholera in birds. This study reports, for the first time, the observed interaction between P. multocida and free-living amoebae. Amoebal trophozoites were coinfected with fowl-cholera-causing P. multocida strain X-73 that expressed the green fluorescent protein (GFP). Using confocal fluorescence microscopy, GFP expressing X-73 was located within the trophozoite. Transmission electron microscopy of coinfection preparations revealed clusters of intact X-73 cells in membrane-bound vacuoles within the trophozoite cytoplasm. A coinfection assay employing gentamicin to kill extracellular bacteria was used to assess the survival and replication of P. multocida within amoebae. In the presence of amoebae, the number of recoverable intracellular X-73 cells increased over a 24-h period; in contrast, X-73 cultured alone in assay medium showed a consistent decline in growth. Cytotoxicity assays and microscopy showed that X-73 was able to lyse and exit the amoebal cells approximately 18 h after coinfection. The observed interaction between P. multocida and amoebae can be considered as an infective process as the bacterium was able to invade, survive, replicate, and lyse the amoebal host. This raises the possibility that similar interactions occur in vivo between P. multocida and host cells. Free-living amoebae are ubiquitous within water and soil environments, and P. multocida has been observed to survive within these same ecosystems. Thus, our findings suggest that the interaction between P. multocida and amoebae may occur within the natural environment.

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Interactions between bovine endothelial cells and Pasteurella multocida: association and invasion

Cited by 22 publications

References 26 publications

Haemophilus parasuis invades porcine brain microvascular endothelial cells

Haemophilus parasuis invades porcine brain microvascular endothelial cells

Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

Interaction of Pasteurella multocida with Free-Living Amoebae

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