Interactions between bacterial populations during degradation of a lubricant base oil

Sonderkamp, Silke; Vomberg, Anja; Schmitz, Christoph; Fassbender, U.; Klinner, Ulrich

doi:10.1111/j.1574-6941.2001.tb00887.x

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…Another possible explanation for not being able to cultivate bacteria from band T is that the strain may be able to grow only where certain compounds essential for its growth are provided by other bacteria. Sonderkamp et al (2001) reported that the growth of a bacterial community could be promoted (accelerated) by the secretion of exoenzymes such as lipase. In their report, a microbial consortium degraded trimethylpropaneoleate, a synthetic fatty acid ester, only when minor enzyme producers secreted lipase.…”

Section: Discussionmentioning

confidence: 99%

A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics

Ichikawa

Hosokawa

Morikawa

et al. 2008

International Biodeterioration & Biodegradation

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A microbial consortium capable of degrading turbine oil (TuO), which consisted mainly of recalcitrant cycloalkanes and isoalkanes, was obtained from a soil sample collected from oil fields using repeated enrichment. When this consortium, named Atsuta A, was cultured in mineral salts medium containing 0.5% (w/v) TuO, it degraded 90% of TuO at 30 °C and pH 7 over 5 days. Although nine bacterial strains were isolated from the Atsuta A consortium, TuO degradation by the individual isolates and by a mixture of them was negligible. The community structure of the consortium, which was investigated by PCR-denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes, changed significantly during the degradation of TuO. Four major bands (F, K, N and T) out of at least 23 visible DGGE bands significantly increased in intensity over time during incubation. The DGGE bands F, K and N corresponded to those of previously isolated species. However, DGGE band T did not correspond to any isolated strain. The 16S rRNA gene sequence collected from band T was 98% homologous to that of an unculturable strain belonging to the γ-Proteobacteria. The degradation of TuO in the consortium may occur by cooperation between the unculturable species corresponding to band T and other strains in the consortium, including species corresponding to bands F, K and N.

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Section: Discussionmentioning

confidence: 99%

A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics

Ichikawa

Hosokawa

Morikawa

et al. 2008

International Biodeterioration & Biodegradation

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“…They are not major constituents of the lubricants, however, and their degradation did not have a profound effect on the volume of lubricant that is lost. Several species of oil‐degrading bacteria (e.g., Acinetobacter lwoffi and Micrococcus roseus ) are capable of rapidly degrading alkanoate esters (octanoate and decanoate) of 1,1,1‐TMP [18–21]. These bacteria are unable to oxidize the tertiary alcohol (1,1,1‐TMP) or to use it as a carbon source; hence, 1,1,1‐TMP is found to accumulate.…”

Section: Discussionmentioning

confidence: 99%

“…Degradation of synthetic lubricants has been studied only under controlled laboratory conditions (e.g., Commission of European Community L‐33‐T‐82 biodegradability test, Brussels, Belgium) and on experimental soil plots [13,17–21]. Laboratory tests sometimes over‐ or underestimate the natural rates, presumably because these tests do not accurately mimic natural conditions (e.g., temperature, currents, nutrients).…”

Section: Introductionmentioning

confidence: 99%

In situ lubricant degradation in Antarctic marine sediments. 1. Short‐term changes

Thompson

Davies

Goldsworthy

et al. 2006

Enviro Toxic and Chemistry

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A large-scale, in situ experiment was set up near the Bailey Peninsula area (Casey Station, East Antarctica) to monitor the natural attenuation of synthetic lubricants in marine sediments over five years. Here, we report the short-term changes after 5 and 56 weeks. The lubricants tested were an unused and used Mobil lubricant (0W/40; Exxon Mobil, Irving, TX, USA) and a biodegradable alternative (0W/20; Fuchs Lubricants, Harvey, IL, USA). Clean sediment was collected, contaminated with the lubricants, and deployed by divers onto the seabed in a randomized block design. The sampled sediments were analyzed by gas chromatography-flame-ionization detector and gas chromatography-mass spectrometry with selective ion monitoring. The base fluid of all lubricant treatments did not decrease significantly after 56 weeks in situ. Alkanoate esters of 1,1,1-tris(hydroxymethyl)propane in the biodegradable and unused lubricants were degraded extensively in situ; however, these esters constituted only a minor proportion of the lubricant volume. The additives, alkylated naphthalenes and substituted diphenylamines, were fairly resistant to degradation, which is of environmental concern because of their toxicity. The biodegradable lubricant did not break down to recognized biodegradable thresholds and, as such, should not be classified as biodegradable under Antarctic marine conditions. A separate experiment was conducted to determine the influence of sediment preparation and deployment on compound ratios within the lubricants, and we found that preparation and deployment of the contaminated sediments had only a minor effect on compound recovery. Further monitoring of this in situ experiment will provide much needed information about the long-term natural attenuation of lubricants.

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“…These results suggest that all or some strains involved in the TuO degradation are included in the IM and PM mixtures, and that an appropriate bacterial community structure is required for TuO degradation. Sonderkamp et al (2001) reported that the secretion of exoenzymes promoted the metabolism of trimethylpropaneoleate in a bacterial 19 community, which suggests that some strains may only exhibit a capacity to degrade TuO, when some essential substances, such as extracellular enzymes, were provided by other bacterial strains in the community Interestingly, four strains of the PM mixture were tentatively identified as being different strains of Pseudomonas putida (Table 3). Pseudomonas species are known as fastgrowing bacteria, which rapidly grow under laboratory conditions (Sayler and Ripp 2000).…”

Section: Degradation Of Tuo By Isolates From the Tank-2 Consortium Anmentioning

confidence: 99%

Establishment and characterization of turbine oil-degrading bacterial consortia

Hosokawa

Sakaguchi

Okuyama

2010

International Biodeterioration & Biodegradation

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A microbial consortium capable of degrading turbine oil (TuO) consisting mainly of recalcitrant branched and polycyclic alkanes, has been established using a sludge sample collected from an oil-water separation tank of a hydraulic plant, by repeated enrichment cultivation. When this consortium, named the tank-2 consortium, was cultivated in a minimal salts medium containing 0.5% TuO, it degraded approximately 90% of TuO at 30 °C under shaking at 160 rpm in five days. Dur ing cultivation, the consor tium for med cell aggregates. Four teen bacter ial species were isolated from the consortium. Although the mixtures of all of the isolated str ains (IM) and predominant str ains selected from the IM (PM) degr aded approximately 60% of the TuO maximally in seven days after repeated enr ichment, the degradation r ate was not maintained. The decreased r ate of TuO degradation in the PM was recovered by inoculation with a mixture (AM) consisting of isolates involved in the cell aggregate for mation in the 3 tank-2 consor tium, suggesting that the for mation of bacter ial aggregates is an important factor for the degradation of TuO by the bacter ial consortia. Interestingly, the for mation of bacter ial aggregates was only obser ved when cyclic alkanes were added to the consortium as a source of car bon. The cell sur face hydrophobicity of the bacter ial aggregates was significantly high (77.6% ). These results suggest that the growth of bacter ia with high cell hydrophobicity can be induced by the cyclic alkanes fr action of TuO, and that the for mation of aggregates facilitates cells to for m contact with the recalcitr ant components of TuO and uptakes these components.

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Interactions between bacterial populations during degradation of a lubricant base oil

Cited by 13 publications

References 28 publications

A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics

A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics

In situ lubricant degradation in Antarctic marine sediments. 1. Short‐term changes

Establishment and characterization of turbine oil-degrading bacterial consortia

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