Interactions between Bacteria And Aspen Defense Chemicals at the Phyllosphere – Herbivore Interface

Mason, Charles J.; Lowe‐Power, Tiffany M.; Rubert‐Nason, Kennedy F.; Lindroth, Richard L.; Raffa, Kenneth F.

doi:10.1007/s10886-016-0677-z

Cited by 42 publications

(30 citation statements)

References 61 publications

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“…However, some other reported cellulose-degrading bacteria, such as Enterobacter and Pseudomonas ( Bayer, Shoham & Lamed, 2006 ; Muhammad et al, 2017 ), have no significant correlation with cellulose and hemicellulose degradation, and the presence of these bacteria may contribute to eliminating the differences in cellulose digestibility in this study. Acinetobacter participates in host food digestion, degrades harmful compounds, and plays a role in nitrogen transformation ( Briones-Roblero et al, 2016 ; Liu et al, 2016 ; Mason et al, 2016 ). Existing studies have shown that Wolbachia plays an important role in the reproductive ability of host insects ( Hancock et al, 2011 ), and whether this genus has an effect on the reproduction of grasshoppers requires further attention.…”

Section: Discussionmentioning

confidence: 99%

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Wang¹,

Bai²,

Zheng³

et al. 2020

PeerJ

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Background Grasshoppers are typical phytophagous pests, and they have large appetites with high utilization of plants fibers, the digestion of which may depend on the microorganisms in their intestines. Grasshoppers have the potential to be utilized in bioreactors, which could improve straw utilization efficiency in the future. In this study, we describe the gut microbiome in three species of grasshoppers, Oedaleus decorus asiaticus, Aiolopus tamulus and Shirakiacris shirakii, by constructing a 16S rDNA gene library and analyzed the digestibility of cellulose and hemicellulose in the grasshoppers by using moss black phenol colorimetry and anthrone colorimetry. Results There were 509,436 bacterial OTUs (Operational Taxonomic Units) detected in the guts of all the grasshoppers sampled. Among them, Proteobacteria and Firmicutes were the most common, Aiolopus tamulus had the highest bacterial diversity, and Shirakiacris shirakii had the highest bacterial species richness. The intestinal microflora structure varied between the different species of grasshopper, with Aiolopus tamulus and Shirakiacris shirakii being the most similar. Meanwhile, the time at which grasshopper specimens were collected also led to changes in the intestinal microflora structure in the same species of grasshoppers. Klebsiella may form the core elements of the microflora in the grasshopper intestinal tract. The digestibility of cellulose/hemicellulose among the three species grasshoppers varied (38.01/24.99%, 43.95/17.21% and 44.12/47.62%). LEfSe analysis and Spearman correlation coefficients showed that the hemicellulosic digestibility of Shirakiacris shirakii was significantly higher than that of the other two species of grasshopper, which may be related to the presence of Pseudomonas, Stenotrophomonas, Glutamicibacter, Corynebacterium, and Brachybacterium in Shirakiacris shirakii intestinal tract. Conclusion The intestinal microbial communities of the three grasshoppers species are similar on phylum level, but the dominant genera of different species grasshoppers are different. The cellulose digestibility of the three species of grasshoppers is relatively high, which may be correlated with the presence of some gut microbiome. Increasing the understanding of the structure and function of the grasshopper intestinal microflora will facilitate further research and the utilization of intestinal microorganisms in the future.

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Section: Discussionmentioning

confidence: 99%

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Wang¹,

Bai²,

Zheng³

et al. 2020

PeerJ

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“…When gypsy moth gut microbial communities were enriched in Acinetobacter , growth was enhanced in the presence of phenolic glycosides in diet. Isolates of Acinetobacter were capable of metabolizing phenolic glycosides in culture (Mason, Lowe‐Power, Rubert‐Nason, Lindroth, & Raffa, ), and inhibition of cytochrome P450‐like enzymes reduced metabolism. Trembling aspen ( Populus tremuloides ) with elevated concentrations of phenolic glycosides had a greater relative abundances of Acinetobacter (Mason, Rubert‐Nason, Lindroth, & Raffa, ).…”

Section: Herbivore Co‐option Of Gut‐associated Bacteria To Facilitatementioning

confidence: 99%

Co‐option of microbial associates by insects and their impact on plant–folivore interactions

Mason

Jones

Felton

2018

Plant Cell & Environment

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Plants possess a suite of traits that make them challenging to consume by insect herbivores. Plant tissues are recalcitrant, have low levels of protein, and may be well defended by chemicals. Insects use diverse strategies for overcoming these barriers, including co-opting metabolic activities from microbial associates. In this review, we discuss the co-option of bacteria and fungi in the herbivore gut. We particularly focus upon chewing, folivorous insects (Coleoptera and Lepidoptera) and discuss the impacts of microbial co-option on herbivore performance and plant responses. We suggest that there are two components to microbial co-option: fixed and plastic relationships. Fixed relationships are involved in integral dietary functions and can be performed by microbial enzymes co-opted into the genome or by stably transferred associates. In contrast, the majority of gut symbionts appear to be looser and perform more facultative, context-dependent functions. This more plastic, variable co-option of bacteria likely produces a greater number of insect phenotypes, which interact differently with plant hosts. By altering plant detection of herbivory or mediating insect interactions with plant defensive compounds, microbes can effectively improve herbivore performance in real time within and between generations.

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“…In addition, the gut microbiome is increasingly recognized as a key player in shaping insectÀplant interactions (Hammer & Bowers, 2015). While plants produce repellant and toxic secondary metabolites against herbivores (Mith€ ofer & Boland, 2012;Oliver & Martinez, 2014;Hammer & Bowers, 2015), herbivore-associated microbes may facilitate detoxification of these toxins (Mason et al, 2016). Additionally, insect-associated bacteria may influence the elicitation of defensive reactions in the host plant (Frago et al, 2012;Zhu et al, 2014;Sugio et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Helicoverpa zea gut‐associated bacteria indirectly induce defenses in tomato by triggering a salivary elicitor(s)

et al. 2017

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Insect gut-associated microbes modulating plant defenses have been observed in beetles and piercing-sucking insects, but the role of caterpillar-associated bacteria in regulating plant induced defenses has not been adequately examined. We identified bacteria from the regurgitant of field-collected Helicoverpa zea larvae using 16S ribosomal RNA (rRNA) gene sequencing and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. A combination of biochemical, molecular, and confocal electron microscopy methods were used to determine the role of caterpillar-associated bacteria in mediating defenses in Solanum lycopersicum (tomato). Laboratory-reared H. zea inoculated with one of the bacteria identified in field-collected H. zea, Enterobacter ludwigii, induced expression of the tomato defense-related enzyme polyphenol oxidase and genes regulated by jasmonic acid (JA), whereas the salicylic acid (SA)-responsive pathogenesis-related gene was suppressed. Additionally, saliva and its main component glucose oxidase from inoculated caterpillars played an important role in elevating tomato anti-herbivore defenses. However, there were only low detectable amounts of regurgitant or bacteria on H. zea-damaged tomato leaves. Our results suggest that H. zea gut-associated bacteria indirectly mediate plant-insect interactions by triggering salivary elicitors. These findings provide a proof of concept that introducing gut bacteria to a herbivore may provide a novel approach to pest management through indirect induction of plant resistance.

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Interactions between Bacteria And Aspen Defense Chemicals at the Phyllosphere – Herbivore Interface

Cited by 42 publications

References 61 publications

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Co‐option of microbial associates by insects and their impact on plant–folivore interactions

Helicoverpa zea gut‐associated bacteria indirectly induce defenses in tomato by triggering a salivary elicitor(s)

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