Interactions between autophagy and phytohormone signaling pathways in plants

Liao, Ching‐Yi; Wang, Ping; Yin, Yanhai; Bassham, Diane C.

doi:10.1002/1873-3468.14355

Cited by 12 publications

(6 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially, it was assumed that ethylene and autophagy are not linked based on the lack of leaf senescence recovery phenotype in Arabidopsis plants deficient in both autophagy and Ethylene-Insensitive 2 ( EIN2) genes ( atg5ein2-1 or atg2ein2-1 double mutants; Yoshimoto et al, 2009). However, several studies later suggested that crosstalk between ethylene and autophagy may exist (Liao et al, 2022). First, the transcript abundance of many ethylene biosynthesis and some ethylene-signaling genes was higher in atg5 and atg9 mutants than in WT ( Col-0 ) Arabidopsis plants (Masclaux-Daubresse et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Autophagy Restricts Tomato Fruit Ripening Via a General Role in Ethylene Repression

Kumaran,

Pathak,

Quandoh

et al. 2023

Preprint

View full text Add to dashboard Cite

Autophagy, a cellular degradation pathway, and the phytohormone ethylene function in plant development, senescence, and stress responses. However, the manner of their interaction is mostly unknown. We reasoned that this may be revealed by studying autophagy in a climacteric fruit ripening context, for which ethylene is crucial. During ripening, fruits undergo softening, color change, toxic compound degradation, volatile production, and sugar assembly by fine-tuning synthesis and degradation of their cellular content. For autophagy activity assessment, we analyzed autophagy-related 8 (ATG8) lipidation and GFP-ATG8-labeled autophagosome flux in tomato fruit cells. Autophagy activity increased sharply from ripening initiation, climaxed at its middle stage, and declined towards its end, resembling ethylene production dynamics. Silencing the core-autophagy genesSlATG2,SlATG7, andSlATG4separately in mature fruits resulted in early ethylene production and ripening onset, which was abrogated by 1-methylcyclopropene (1-MCP), an ethylene signaling inhibitor. Beyond ripening, Arabidopsisatg5andatg7mutant seedlings exhibited elevated ethylene production and sensitivity to 1-Aminocyclopropane 1-carboxylic acid (ACC), ethylenès precursor, which induces autophagy. This research demonstrates that autophagy limits tomato fruit ripening via a general role in ethylene restriction, opening the path for a mechanistic understanding of autophagy-ethylene crosstalk and harnessing autophagy for fruit shelf-life extension.

show abstract

Section: Introductionmentioning

confidence: 99%

Autophagy Restricts Tomato Fruit Ripening Via a General Role in Ethylene Repression

Kumaran,

Pathak,

Quandoh

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Autophagy is an evolutionarily highly conserved cyclic process that plays a major role in plant growth, development, and stress response [11]. The process of autophagosome formation involves several steps, including signal transduction (induction), isolation membrane formation (nucleation), isolation membrane elongation (elongation), autophagosome completion and transport (maturation), autophagosome-vacuole coupling and fusion (fusion), cargo degradation (degradation), and recycling [12].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated SlATG5 Mutagenesis Reduces the Resistance of Tomato Fruit to Botrytis cinerea

Shu

Xiang

et al. 2023

Foods

View full text Add to dashboard Cite

Tomato fruit is highly susceptible to infection by Botrytis cinerea (B. cinerea), a dominant pathogen, during storage. Recent studies have shown that autophagy is essential for plant defense against biotic and abiotic stresses. Autophagy-related gene 5 (ATG5) plays a key role in autophagosome completion and maturation, and is rapidly induced by B. cinerea, but the potential mechanisms of ATG5 in Solanum lycopersicum (SlATG5) in postharvest tomato fruit resistance to B. cinerea remain unclear. To elucidate the role of SlATG5 in tomato fruit resistant to B. cinerea, CRISPR/Cas9-mediated knockout of SlATG5 was used in this study. The results showed that slatg5 mutants were more vulnerable to B. cinerea and exhibited more severe disease symptoms and lower activities of disease-resistant enzymes, such as chitinase (CHI), β-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO), than the wild type (WT). Furthermore, the study observed that after inoculation with B. cinerea, the relative expression levels of genes related to salicylic acid (SA) signaling, such as SlPR1, SlEDS1, SlPAD4, and SlNPR1, were higher in slatg5 mutants than in WT. Conversely, the relative expression levels of jasmonic acid (JA) signaling-related genes SlLoxD and SlMYC2 were lower in slatg5 mutants than in WT. These findings suggested that SlATG5 positively regulated the resistance response of tomato fruit to B. cinerea by inhibiting the SA signaling pathway and activating the JA signaling pathway.

show abstract

“…Cargoes including single macromolecules, large macromolecular complexes such as ribosomes and proteasomes, protein aggregates, damaged or whole organelles, and even invading pathogens can be eliminated through autophagy in bulk or selectively ( Liu and Bassham, 2012 ; Marshall and Vierstra, 2018 ). This autophagic process is critical for removing non-functional molecules within the cell and replenishing the nutrient sources for new growth, and it is also important for regulating specific biological processes or signaling pathways by selectively degrading specific cargoes ( Zhou et al, 2014 ; Nolan et al, 2017 ; Zhuang et al, 2018 ; Xia et al, 2020 ; Lin et al, 2021 ; Luo et al, 2021 ; Wang et al, 2021 ; Liao et al, 2022 ). Thus, autophagy plays important roles in plant growth and development and stress responses to facilitate plant tolerance and survival under unfavorable conditions ( Marshall and Vierstra, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

FERONIA functions through Target of Rapamycin (TOR) to negatively regulate autophagy

et al. 2022

Self Cite

View full text Add to dashboard Cite

FERONIA (FER) receptor kinase plays versatile roles in plant growth and development, biotic and abiotic stress responses, and reproduction. Autophagy is a conserved cellular recycling process that is critical for balancing plant growth and stress responses. Target of Rapamycin (TOR) has been shown to be a master regulator of autophagy. Our previous multi-omics analysis with loss-of-function fer-4 mutant implicated that FER functions in the autophagy pathway. We further demonstrated here that the fer-4 mutant displayed constitutive autophagy, and FER is required for TOR kinase activity measured by S6K1 phosphorylation and by root growth inhibition assay to TOR kinase inhibitor AZD8055. Taken together, our study provides a previously unknown mechanism by which FER functions through TOR to negatively regulate autophagy.

show abstract

Interactions between autophagy and phytohormone signaling pathways in plants

Cited by 12 publications

References 152 publications

Autophagy Restricts Tomato Fruit Ripening Via a General Role in Ethylene Repression

Autophagy Restricts Tomato Fruit Ripening Via a General Role in Ethylene Repression

CRISPR/Cas9-Mediated SlATG5 Mutagenesis Reduces the Resistance of Tomato Fruit to Botrytis cinerea

FERONIA functions through Target of Rapamycin (TOR) to negatively regulate autophagy

Contact Info

Product

Resources

About