Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response in<i>Solanum lycopersicum</i>

Cheng, Fei; Yin, Lingling; Zhou, Jie; Xia, Xiaojian; Shi, Kai; Yu, Jingquan; Zhou, Yanhong; Foyer, Christine H.

doi:10.1093/jxb/erw013

Cited by 36 publications

(32 citation statements)

References 51 publications

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“…Use of TEM is a valid and important method for the quantitative analysis of autophagosomes (Cheng et al ., ; Klionsky et al ., ). Our transgenic lines had more autophagosomes than WT under drought stress, a finding that was in line with the decreased accumulation of oxidized insoluble proteins under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Improvement of drought tolerance by overexpressing MdATG18a is mediated by modified antioxidant system and activated autophagy in transgenic apple

Sun

Wang

Jia

et al. 2017

Plant Biotechnology Journal

197

144

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SummaryAutophagy is a major and conserved pathway for delivering and recycling unwanted proteins or damaged organelles to be degraded in the vacuoles. AuTophaGy‐related (ATG) protein 18a has been established as one of the essential components for autophagy occurrence in Arabidopsis thaliana. We previously cloned the ATG18a homolog from Malus domestica (MdATG18a) and monitored its responsiveness to various abiotic stresses at the transcriptional level. However, it is still unclear what its function is under abiotic stress in apple. Here, we found that heterologous expression of MdATG18a in tomato plants markedly enhanced their tolerance to drought. Overexpression (OE) of that gene in apple plants improved their drought tolerance as well. Under drought conditions, the photosynthesis rate and antioxidant capacity were significantly elevated in OE lines when compared with the untransformed wild type (WT). Transcript levels of other important apple ATG genes were more strongly up‐regulated in transgenic MdATG18a OE lines than in the WT. The percentage of insoluble protein in proportion to total protein was lower and less oxidized protein accumulated in the OE lines than in the WT under drought stress. This was probably due to more autophagosomes being formed in the former. These results demonstrate that overexpression of MdATG18a in apple plants enhances their tolerance to drought stress, probably because of greater autophagosome production and a higher frequency of autophagy. Those processes help degrade protein aggregation and limit the oxidation damage, thereby suggesting that autophagy plays important roles in the drought response.

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Section: Discussionmentioning

confidence: 99%

Improvement of drought tolerance by overexpressing MdATG18a is mediated by modified antioxidant system and activated autophagy in transgenic apple

Sun

Wang

Jia

et al. 2017

Plant Biotechnology Journal

197

144

View full text Add to dashboard Cite

show abstract

“…Our group previously described that heat stress induces the expression of autophagy‐related (ATG) genes and the accumulation of autophagosomes in tomato plants . Virus‐induced gene silencing (VIGS) of several tomato ATGs resulted in an increased sensitivity to heat stress, suggesting the involvement of autophagy in the heat stress response in tomato plants . Recently, Wang et al.…”

Section: Discussionmentioning

confidence: 99%

“…42 Virusinduced gene silencing (VIGS) of several tomato ATGs resulted in an increased sensitivity to heat stress, suggesting the involvement of autophagy in the heat stress response in tomato plants. 4 Recently, Wang et al also reported that melatonin enhanced the occurrence of autophagy in response of the Arabidopsis to methyl viologen (MV)-induced oxidative stress. 37 Here, we found that the expression of several ATGs and the production of autophagosomes were further induced by melatonin pretreatment and overexpression of ASMT under heat stress ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to being a crucial component of plant cell structure, proteins are directly implicated in plant stress metabolism. Heat stress causes the oxidation and misfolding of proteins in plant cells . Therefore, enhancement of thermotolerance in plant preferentially involves the adoption strategies that protect proteins from oxidation and misfolding.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin enhances thermotolerance by promoting cellular protein protection in tomato plants

Cai

Zhang

et al. 2016

Journal of Pineal Research

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229

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Melatonin is a pleiotropic signaling molecule that provides physiological protection against diverse environmental stresses in plants. Nonetheless, the mechanisms for melatonin-mediated thermotolerance remain largely unknown. Here, we report that endogenous melatonin levels increased with a rise in ambient temperature and that peaked at 40°C. Foliar pretreatment with an optimal dose of melatonin (10 μmol/L) or the overexpression of N-acetylserotonin methyltransferase (ASMT) gene effectively ameliorated heat-induced photoinhibition and electrolyte leakage in tomato plants. Both exogenous melatonin treatment and endogenous melatonin manipulation by overexpression of ASMT decreased the levels of insoluble and ubiquitinated proteins, but enhanced the expression of heat-shock proteins (HSPs) to refold denatured and unfolded proteins under heat stress. Meanwhile, melatonin also induced expression of several ATG genes and formation of autophagosomes to degrade aggregated proteins under the same stress. Proteomic profile analyses revealed that protein aggregates for a large number of biological processes accumulated in wild-type plants. However, exogenous melatonin treatment or overexpression of ASMT reduced the accumulation of aggregated proteins. Aggregation responsive proteins such as HSP70 and Rubisco activase were preferentially accumulated and ubiquitinated in wild-type plants under heat stress, while melatonin mitigated heat stress-induced accumulation and ubiquitination of aggregated proteins. These results suggest that melatonin promotes cellular protein protection through induction of HSPs and autophagy to refold or degrade denatured proteins under heat stress in tomato plants.

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“…Autophagosomes assemble in the cytosol to remove oxidatively modified structures as large as organelles. Interestingly, impaired chloroplast antioxidant function is sufficient to trigger this pathway (Cheng et al, 2016).…”

Section: Longer Term Signaling Downstream Of Rosmentioning

confidence: 99%

Intracellular Redox Compartmentation and ROS-Related Communication in Regulation and Signaling

Noctor

Foyer²

2016

Plant Physiol.

298

161

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Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response inSolanum lycopersicum

Abstract: Highlight2-Cys peroxiredoxins fulfil a pivotal role in heat stress tolerance in Solanum lycopersicum via interactions with ascorbate-dependent pathways and autophagy.

Cited by 36 publications

References 51 publications

Improvement of drought tolerance by overexpressing MdATG18a is mediated by modified antioxidant system and activated autophagy in transgenic apple

Improvement of drought tolerance by overexpressing MdATG18a is mediated by modified antioxidant system and activated autophagy in transgenic apple

Melatonin enhances thermotolerance by promoting cellular protein protection in tomato plants

Intracellular Redox Compartmentation and ROS-Related Communication in Regulation and Signaling

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